(Circular) (Six-base) MAPSORT of: pFN21AE1300.seq Check: 4997 from: 1 to: 6421 pFN21AE1300 6421 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2079 4231 278 Size: 53 83 186 936 543 2152 2468 Fragments arranged by size: 2468 2152 936 543 186 83 53 AccI GT'mk_AC Cuts at: 1937 3764 1937 Size: 1827 4594 Acc65I G'GTAC_C Cuts at: 2633 3742 2633 Size: 1109 5312 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3180 3342 4484 5868 6136 1632 Size: 1548 162 1142 1384 268 1917 Fragments arranged by size: 1917 1548 1384 1142 268 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 2025 5320 6297 1203 Size: 822 3295 977 1327 Fragments arranged by size: 3295 1327 977 822 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 5150 5150 Size: 6421 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6421 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6421 AlwNI CAG_nnn'CTG Cuts at: 2143 4164 5736 2143 Size: 2021 1572 2828 Fragments arranged by size: 2828 2021 1572 ApaI G_GGCC'C Cuts at: 2669 2927 3395 2669 Size: 258 468 5695 Fragments arranged by size: 5695 468 258 ApaLI G'TGCA_C Cuts at: 2207 4478 5634 2207 Size: 2271 1156 2994 Fragments arranged by size: 2994 2271 1156 ApoI r'AATT_y Cuts at: 1493 3730 3956 4108 6217 1493 Size: 2237 226 152 2109 1697 Fragments arranged by size: 2237 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4975 160 Size: 872 3943 1606 Fragments arranged by size: 3943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 2110 2158 2303 2326 3698 3746 1870 Size: 73 167 48 145 23 1372 48 4545 Fragments arranged by size: 4545 1372 167 145 73 48 48 23 BamHI G'GATC_C Cuts at: 3751 4118 3751 Size: 367 6054 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2406 2543 2633 2650 3564 Size: 325 217 627 619 137 90 17 914 Cuts at: 3564 3742 5197 618 Size: 178 1455 1842 Fragments arranged by size: 1842 1455 914 627 619 325 217 178 137 90 17 BanII G_rGCy'C Cuts at: 1391 2157 2669 2738 2859 2888 2927 3017 3072 Size: 766 512 69 121 29 39 90 55 Cuts at: 3072 3395 3624 3740 1391 Size: 323 229 116 4072 Fragments arranged by size: 4072 766 512 323 229 121 116 90 69 55 39 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3430 3585 3658 928 Size: 971 1531 155 73 3691 Fragments arranged by size: 3691 1531 971 155 73 BbvCI CC'TCA_GC Cuts at: 2345 2661 3150 3533 2345 Size: 316 489 383 5233 Fragments arranged by size: 5233 489 383 316 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 1903 Size: 34 2675 34 3678 Fragments arranged by size: 3678 2675 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4313 5523 4313 Size: 1210 5211 BclI T'GATC_A Cuts at: 1548 1548 Size: 6421 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 2832 2904 Size: 107 122 71 631 605 452 708 72 Cuts at: 2904 3489 5032 136 Size: 585 1543 1525 Fragments arranged by size: 1543 1525 708 631 605 585 452 122 107 72 71 BglII A'GATC_T Cuts at: 6416 6416 Size: 6421 BlpI GC'TnA_GC Cuts at: 3838 3838 Size: 6421 Bme1580I G_kGCm'C Cuts at: 1790 2211 2297 2655 2669 2927 3395 4482 5638 Size: 421 86 358 14 258 468 1087 1156 Cuts at: 5638 1790 Size: 2573 Fragments arranged by size: 2573 1156 1087 468 421 358 258 86 14 BmgBI CAC'GTC Cuts at: 1318 2212 1318 Size: 894 5527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3194 4152 5110 Size: 416 53 83 399 24 1777 958 958 Cuts at: 5110 442 Size: 1753 Fragments arranged by size: 1777 1753 958 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2825 Size: 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2450 2509 2536 3395 5081 1239 Size: 911 300 59 27 859 1686 2579 Fragments arranged by size: 2579 1686 911 859 300 59 27 Bpu10I CC'TnA_GC Cuts at: 2094 2345 2661 3150 3533 2094 Size: 251 316 489 383 4982 Fragments arranged by size: 4982 489 383 316 251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 4546 5411 5709 2843 Size: 1703 865 298 3555 Fragments arranged by size: 3555 1703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2796 3218 5084 882 Size: 617 1297 422 1866 2219 Fragments arranged by size: 2219 1866 1297 617 422 BsaAI yAC'GTr Cuts at: 493 1204 4212 493 Size: 711 3008 2702 Fragments arranged by size: 3008 2702 711 BsaBI GATnn'nnATC Cuts at: 2267 6415 2267 Size: 4148 2273 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 3213 3366 4228 Size: 53 83 186 936 543 1137 153 862 Cuts at: 4228 4610 5245 275 Size: 382 635 1451 Fragments arranged by size: 1451 1137 936 862 635 543 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 4854 5252 5526 5673 6084 4854 Size: 398 274 147 411 5191 Fragments arranged by size: 5191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6196 Fragments arranged by size: 6196 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 3022 1186 Size: 1633 203 4585 Fragments arranged by size: 4585 1633 203 BseYI C'CCAG_C Cuts at: 2015 2398 2449 2919 5624 6120 2015 Size: 383 51 470 2705 496 2316 Fragments arranged by size: 2705 2316 496 470 383 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2305 1163 Size: 573 569 5279 Fragments arranged by size: 5279 573 569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3871 4632 5660 1540 Size: 275 186 1870 761 1028 2301 Fragments arranged by size: 2301 1870 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2211 2859 4482 4567 5638 2100 Size: 111 648 1623 85 1071 2883 Fragments arranged by size: 2883 1623 1071 648 111 85 BsmI GAATG_Cn' Cuts at: 3932 4025 3932 Size: 93 6328 BsmBI CGTCTCn'nnnn_ Cuts at: 2554 2554 Size: 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2157 2211 2297 2655 2669 2738 Size: 399 310 57 54 86 358 14 69 Cuts at: 2738 2859 2888 2927 3017 3072 3395 3624 3740 Size: 121 29 39 90 55 323 229 116 Cuts at: 3740 4482 4567 5638 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 323 310 229 121 116 90 86 85 69 57 55 54 39 29 14 BspEI T'CCGG_A Cuts at: 6084 6084 Size: 6421 BspHI T'CATG_A Cuts at: 4310 6040 4310 Size: 1730 4691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 3776 844 Size: 1307 1625 3489 Fragments arranged by size: 3489 1625 1307 BsrBI CCG'CTC Cuts at: 1394 1823 4308 1394 Size: 429 2485 3507 Fragments arranged by size: 3507 2485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2647 3499 4916 5090 65 Size: 1183 1399 852 1417 174 1396 Fragments arranged by size: 1417 1399 1396 1183 852 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5065 5252 1700 Size: 110 3255 187 2869 Fragments arranged by size: 3255 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6421 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 4481 5493 4481 Size: 1012 5409 BstAPI GCAn_nnn'nTGC Cuts at: 794 2292 2368 3498 794 Size: 1498 76 1130 3717 Fragments arranged by size: 3717 1498 1130 76 BstBI TT'CG_AA Cuts at: 5225 5225 Size: 6421 BstEII G'GTnAC_C Cuts at: 1184 3349 1184 Size: 2165 4256 BstXI CCAn_nnnn'nTGG Cuts at: 3494 3494 Size: 6421 BstYI r'GATC_y Cuts at: 1972 3639 3702 3751 4118 4503 4520 5972 6058 Size: 1667 63 49 367 385 17 1452 86 Cuts at: 6058 6070 6416 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1667 1452 385 367 346 86 63 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2523 2815 3354 513 Size: 552 939 48 471 292 539 3580 Fragments arranged by size: 3580 939 552 539 471 292 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2593 2641 487 Size: 683 56 1367 48 4267 Fragments arranged by size: 4267 1367 683 56 48 BtsI GCAGTG_nn' Cuts at: 3937 4731 4751 3937 Size: 794 20 5607 Fragments arranged by size: 5607 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3217 3252 3445 3480 529 Size: 35 2653 35 193 35 3470 Fragments arranged by size: 3470 2653 193 35 35 35 DraI TTT'AAA Cuts at: 3725 3725 Size: 6421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5428 809 Size: 507 4112 1802 Fragments arranged by size: 4112 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2055 2728 Size: 54 1425 50 275 18 219 6 673 Cuts at: 2728 2763 2793 3000 3087 3184 3466 3592 3868 Size: 35 30 207 87 97 282 126 276 Cuts at: 3868 4757 6386 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 673 282 276 275 219 207 126 97 87 54 50 43 35 30 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2816 2876 3518 4351 1353 Size: 869 594 60 642 833 3423 Fragments arranged by size: 3423 869 833 642 594 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5008 5382 5528 387 Size: 1064 138 3419 374 146 1280 Fragments arranged by size: 3419 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2857 2857 Size: 6421 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2450 2509 2536 3180 3342 3395 Size: 393 518 300 59 27 644 162 53 Cuts at: 3395 4484 5081 5868 6136 1239 Size: 1089 597 787 268 1524 Fragments arranged by size: 1524 1089 787 644 597 518 393 300 268 162 59 53 27 EcoNI CCTnn'n_nnAGG Cuts at: 2468 2591 3604 2468 Size: 123 1013 5285 Fragments arranged by size: 5285 1013 123 EcoO109I rG'GnC_Cy Cuts at: 2187 2322 2473 2665 2666 2685 2716 2923 2924 Size: 135 151 192 1 19 31 207 1 Cuts at: 2924 3190 3391 3392 2187 Size: 266 201 1 5216 Fragments arranged by size: 5216 266 207 201 192 151 135 31 19 1 1 1 EcoRI G'AATT_C Cuts at: 3730 4108 3730 Size: 378 6043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3281 3281 Size: 6421 FspI TGC'GCA Cuts at: 2267 4927 2267 Size: 2660 3761 FspAI rTGC'GCAy Cuts at: 2267 2267 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 2496 3589 5568 1678 Size: 818 1093 1979 2531 Fragments arranged by size: 2531 1979 1093 818 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5062 5094 5136 5168 1373 Size: 32 135 32 3490 32 42 32 2626 Fragments arranged by size: 3490 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3765 4011 669 Size: 988 281 1827 246 3079 Fragments arranged by size: 3079 1827 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6421 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KpnI G_GTAC'C Cuts at: 2637 3746 2637 Size: 1109 5312 MfeI C'AATT_G Cuts at: 4020 4020 Size: 6421 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5535 5719 1305 Size: 4230 184 2007 Fragments arranged by size: 4230 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2051 2765 2795 3089 3468 Size: 54 1425 343 219 714 30 294 379 Cuts at: 3468 10 Size: 2963 Fragments arranged by size: 2963 1425 714 379 343 294 219 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 3656 4379 4738 4897 518 Size: 648 2490 723 359 159 2042 Fragments arranged by size: 2490 2042 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2281 2566 2709 3006 3356 3532 3558 3837 Size: 675 285 143 297 350 176 26 279 Cuts at: 3837 4514 5662 5907 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 675 350 297 285 279 245 176 143 26 NcoI C'CATG_G Cuts at: 513 1065 2004 2052 513 Size: 552 939 48 4882 Fragments arranged by size: 4882 939 552 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 6421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 2029 3685 3779 5324 1132 Size: 97 800 1656 94 1545 2229 Fragments arranged by size: 2229 1656 1545 800 97 94 PasI CC'CwG_GG Cuts at: 1348 2412 2914 3487 1348 Size: 1064 502 573 4282 Fragments arranged by size: 4282 1064 573 502 PciI A'CATG_T Cuts at: 2025 5320 2025 Size: 3295 3126 PflMI CCAn_nnn'nTGG Cuts at: 1108 2529 1108 Size: 1421 5000 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4490 4522 6003 6035 4490 Size: 32 1481 32 4876 Fragments arranged by size: 4876 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2187 2187 Size: 6421 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6421 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PspOMI G'GGCC_C Cuts at: 2665 2923 3391 2665 Size: 258 468 5695 Fragments arranged by size: 5695 468 258 PstI C_TGCA'G Cuts at: 830 2373 2897 3773 830 Size: 1543 524 876 3478 Fragments arranged by size: 3478 1543 876 524 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6421 PvuII CAG'CTG Cuts at: 2709 3532 2709 Size: 823 5598 SacI G_AGCT'C Cuts at: 2859 2859 Size: 6421 SacII CC_GC'GG Cuts at: 3357 3357 Size: 6421 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SbfI CC_TGCA'GG Cuts at: 2373 3773 2373 Size: 1400 5021 ScaI AGT'ACT Cuts at: 4669 6381 4669 Size: 1712 4709 SexAI A'CCwGG_T Cuts at: 2646 2646 Size: 6421 SfcI C'TryA_G Cuts at: 826 1045 2369 2893 3769 4904 5585 5776 826 Size: 219 1324 524 876 1135 681 191 1471 Fragments arranged by size: 1471 1324 1135 876 681 524 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 2112 2305 2328 3700 3748 1872 Size: 240 193 23 1372 48 4545 Fragments arranged by size: 4545 1372 240 193 48 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 2158 2858 4525 5426 5688 820 Size: 197 926 215 700 1667 901 262 1553 Fragments arranged by size: 1667 1553 926 901 700 262 215 197 SnaBI TAC'GTA Cuts at: 493 4212 493 Size: 3719 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 3779 1132 Size: 2647 3774 SspI AAT'ATT Cuts at: 5 52 4345 5 Size: 47 4293 2081 Fragments arranged by size: 4293 2081 47 StuI AGG'CCT Cuts at: 1517 2420 2441 3101 1517 Size: 903 21 660 4837 Fragments arranged by size: 4837 903 660 21 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2587 3292 3508 3860 513 Size: 552 939 48 535 705 216 352 3074 Fragments arranged by size: 3074 939 705 552 535 352 216 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3552 3634 4448 4465 4618 4803 5248 Size: 1255 1972 82 814 17 153 185 445 Cuts at: 5248 325 Size: 1498 Fragments arranged by size: 1972 1498 1255 814 445 185 153 82 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4667 6379 96 Size: 274 80 33 51 1443 2690 1712 138 Fragments arranged by size: 2690 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6381 6413 6381 Size: 32 6389 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6421 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2484 2484 Size: 6421 XhoI C'TCGA_G Cuts at: 1943 2158 1943 Size: 215 6206 XmaI C'CCGG_G Cuts at: 1870 2110 2303 2326 3698 3746 1870 Size: 240 193 23 1372 48 4545 Fragments arranged by size: 4545 1372 240 193 48 23 XmnI GAAnn'nnTTC Cuts at: 4550 4550 Size: 6421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2077 4229 276 Size: 53 83 186 936 543 2152 2468 Fragments arranged by size: 2468 2152 936 543 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI