(Linear) (Six-base) MAPSORT of: KIEE1300.seq Check: 9365 from: 1 to: 1716 KIEE1300.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AatII G_ACGT'C Cuts at: 0 74 1716 Size: 74 1642 Acc65I G'GTAC_C Cuts at: 0 628 1716 Size: 628 1088 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1175 1337 1716 Size: 1175 162 379 Fragments arranged by size: 1175 379 162 AflIII A'CryG_T Cuts at: 0 20 1716 Size: 20 1696 AlwNI CAG_nnn'CTG Cuts at: 0 138 1716 Size: 138 1578 ApaI G_GGCC'C Cuts at: 0 664 922 1390 1716 Size: 664 258 468 326 Fragments arranged by size: 664 468 326 258 ApaLI G'TGCA_C Cuts at: 0 202 1716 Size: 202 1514 AvaI C'yCGr_G Cuts at: 0 105 153 298 321 1693 1716 Size: 105 48 145 23 1372 23 Fragments arranged by size: 1372 145 105 48 23 23 BanI G'GyrC_C Cuts at: 0 401 538 628 645 1559 1716 Size: 401 137 90 17 914 157 Fragments arranged by size: 914 401 157 137 90 17 BanII G_rGCy'C Cuts at: 0 152 664 733 854 883 922 1012 1067 Size: 152 512 69 121 29 39 90 55 Cuts at: 1067 1390 1619 1716 Size: 323 229 97 Fragments arranged by size: 512 323 229 152 121 97 90 69 55 39 29 BbsI GAAGACnn'nnnn_ Cuts at: 0 1425 1580 1653 1716 Size: 1425 155 73 63 Fragments arranged by size: 1425 155 73 63 BbvCI CC'TCA_GC Cuts at: 0 340 656 1145 1528 1716 Size: 340 316 489 383 188 Fragments arranged by size: 489 383 340 316 188 BglI GCCn_nnn'nGGC Cuts at: 0 119 827 899 1484 1716 Size: 119 708 72 585 232 Fragments arranged by size: 708 585 232 119 72 Bme1580I G_kGCm'C Cuts at: 0 206 292 650 664 922 1390 1716 Size: 206 86 358 14 258 468 326 Fragments arranged by size: 468 358 326 258 206 86 14 BmgBI CAC'GTC Cuts at: 0 207 1716 Size: 207 1509 BmrI ACTGGGnnnn_n' Cuts at: 0 1189 1716 Size: 1189 527 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 820 1716 Size: 820 896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 445 504 531 1390 1716 Size: 145 300 59 27 859 326 Fragments arranged by size: 859 326 300 145 59 27 Bpu10I CC'TnA_GC Cuts at: 0 89 340 656 1145 1528 1716 Size: 89 251 316 489 383 188 Fragments arranged by size: 489 383 316 251 188 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 838 1716 Size: 838 878 BsaI GGTCTCn'nnnn_ Cuts at: 0 791 1213 1716 Size: 791 422 503 Fragments arranged by size: 791 503 422 BsaBI GATnn'nnATC Cuts at: 0 262 1716 Size: 262 1454 BsaHI Gr'CG_yC Cuts at: 0 71 1208 1361 1716 Size: 71 1137 153 355 Fragments arranged by size: 1137 355 153 71 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 814 1017 1716 Size: 814 203 699 Fragments arranged by size: 814 699 203 BseYI C'CCAG_C Cuts at: 0 10 393 444 914 1716 Size: 10 383 51 470 802 Fragments arranged by size: 802 470 383 51 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 1716 Size: 300 1416 BsiHKAI G_wGCw'C Cuts at: 0 95 206 854 1716 Size: 95 111 648 862 Fragments arranged by size: 862 648 111 95 BsmBI CGTCTCn'nnnn_ Cuts at: 0 549 1716 Size: 549 1167 Bsp1286I G_dGCh'C Cuts at: 0 95 152 206 292 650 664 733 854 Size: 95 57 54 86 358 14 69 121 Cuts at: 854 883 922 1012 1067 1390 1619 1716 Size: 29 39 90 55 323 229 97 Fragments arranged by size: 358 323 229 121 97 95 90 86 69 57 55 54 39 29 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 146 1716 Size: 146 1570 BsrDI GCAATG_nn' Cuts at: 0 642 1494 1716 Size: 642 852 222 Fragments arranged by size: 852 642 222 BstAPI GCAn_nnn'nTGC Cuts at: 0 287 363 1493 1716 Size: 287 76 1130 223 Fragments arranged by size: 1130 287 223 76 BstEII G'GTnAC_C Cuts at: 0 1344 1716 Size: 1344 372 BstXI CCAn_nnnn'nTGG Cuts at: 0 1489 1716 Size: 1489 227 BstYI r'GATC_y Cuts at: 0 1634 1697 1716 Size: 1634 63 19 Fragments arranged by size: 1634 63 19 BtgI C'CryG_G Cuts at: 0 47 518 810 1349 1716 Size: 47 471 292 539 367 Fragments arranged by size: 539 471 367 292 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 588 636 1716 Size: 588 48 1080 Fragments arranged by size: 1080 588 48 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1212 1247 1440 1475 1716 Size: 1212 35 193 35 241 Fragments arranged by size: 1212 241 193 35 35 EaeI y'GGCC_r Cuts at: 0 44 50 723 758 788 995 1082 1179 Size: 44 6 673 35 30 207 87 97 Cuts at: 1179 1461 1587 1716 Size: 282 126 129 Fragments arranged by size: 673 282 207 129 126 97 87 44 35 30 6 EarI CTCTTCn'nnn_ Cuts at: 0 217 811 871 1513 1716 Size: 217 594 60 642 203 Fragments arranged by size: 642 594 217 203 60 EcoICRI GAG'CTC Cuts at: 0 852 1716 Size: 852 864 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 445 504 531 1175 1337 1390 1716 Size: 145 300 59 27 644 162 53 326 Fragments arranged by size: 644 326 300 162 145 59 53 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 463 586 1599 1716 Size: 463 123 1013 117 Fragments arranged by size: 1013 463 123 117 EcoO109I rG'GnC_Cy Cuts at: 0 182 317 468 660 661 680 711 918 Size: 182 135 151 192 1 19 31 207 Cuts at: 918 919 1185 1386 1387 1716 Size: 1 266 201 1 329 Fragments arranged by size: 329 266 207 201 192 182 151 135 31 19 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1276 1716 Size: 1276 440 FspI TGC'GCA Cuts at: 0 262 1716 Size: 262 1454 FspAI rTGC'GCAy Cuts at: 0 262 1716 Size: 262 1454 HaeII r_GCGC'y Cuts at: 0 491 1584 1716 Size: 491 1093 132 Fragments arranged by size: 1093 491 132 KpnI G_GTAC'C Cuts at: 0 632 1716 Size: 632 1084 MscI TGG'CCA Cuts at: 0 46 760 790 1084 1463 1716 Size: 46 714 30 294 379 253 Fragments arranged by size: 714 379 294 253 46 30 MslI CAynn'nnrTG Cuts at: 0 1651 1716 Size: 1651 65 MspA1I CmG'CkG Cuts at: 0 276 561 704 1001 1351 1527 1553 1716 Size: 276 285 143 297 350 176 26 163 Fragments arranged by size: 350 297 285 276 176 163 143 26 NcoI C'CATG_G Cuts at: 0 47 1716 Size: 47 1669 NspI r_CATG'y Cuts at: 0 24 1680 1716 Size: 24 1656 36 Fragments arranged by size: 1656 36 24 PasI CC'CwG_GG Cuts at: 0 407 909 1482 1716 Size: 407 502 573 234 Fragments arranged by size: 573 502 407 234 PciI A'CATG_T Cuts at: 0 20 1716 Size: 20 1696 PflMI CCAn_nnn'nTGG Cuts at: 0 524 1716 Size: 524 1192 PpuMI rG'GwC_Cy Cuts at: 0 182 1716 Size: 182 1534 PspOMI G'GGCC_C Cuts at: 0 660 918 1386 1716 Size: 660 258 468 330 Fragments arranged by size: 660 468 330 258 PstI C_TGCA'G Cuts at: 0 368 892 1716 Size: 368 524 824 Fragments arranged by size: 824 524 368 PvuII CAG'CTG Cuts at: 0 704 1527 1716 Size: 704 823 189 Fragments arranged by size: 823 704 189 SacI G_AGCT'C Cuts at: 0 854 1716 Size: 854 862 SacII CC_GC'GG Cuts at: 0 1352 1716 Size: 1352 364 SbfI CC_TGCA'GG Cuts at: 0 368 1716 Size: 368 1348 SexAI A'CCwGG_T Cuts at: 0 641 1716 Size: 641 1075 SfcI C'TryA_G Cuts at: 0 364 888 1716 Size: 364 524 828 Fragments arranged by size: 828 524 364 SmaI CCC'GGG Cuts at: 0 107 300 323 1695 1716 Size: 107 193 23 1372 21 Fragments arranged by size: 1372 193 107 23 21 SmlI C'TyrA_G Cuts at: 0 153 853 1716 Size: 153 700 863 Fragments arranged by size: 863 700 153 StuI AGG'CCT Cuts at: 0 415 436 1096 1716 Size: 415 21 660 620 Fragments arranged by size: 660 620 415 21 StyI C'CwwG_G Cuts at: 0 47 582 1287 1503 1716 Size: 47 535 705 216 213 Fragments arranged by size: 705 535 216 213 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1547 1629 1716 Size: 1547 82 87 Fragments arranged by size: 1547 87 82 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 479 1716 Size: 479 1237 XhoI C'TCGA_G Cuts at: 0 153 1716 Size: 153 1563 XmaI C'CCGG_G Cuts at: 0 105 298 321 1693 1716 Size: 105 193 23 1372 23 Fragments arranged by size: 1372 193 105 23 23 ZraI GAC'GTC Cuts at: 0 72 1716 Size: 72 1644 Enzymes that do cut: AatII Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII KpnI MscI MslI MspA1I NcoI NspI PasI PciI PflMI PpuMI PspOMI PstI PvuII SacI SacII SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII XcmI XhoI XmaI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmtI BsaAI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XmnI