################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 9 CGCG CG^CG AfaI 311 GTAC GT^AC Alw44I 579 GTGCAC G^TGCAC Aor51HI 53 AGCGCT AGC^GCT ApaLI 579 GTGCAC G^TGCAC AvaI 26 CTCGGG C^[CT]CG[GA]G AvrII 660 CCTAGG C^CTAGG BalI 894 TGGCCA TGG^CCA BcnI 174 CCGGG CC^[GC]GG BlnI 660 CCTAGG C^CTAGG BlpI 268 GCTAAGC GC^T[ACGT]AGC BpmI 868 CTGGAG CTGGAG(16/14) Bpu1102I 268 GCTAAGC GC^T[ACGT]AGC BsmAI 440 GAGAC GTCTC(1/5) BsmFI 657 GTCCC GGGAC(10/14) BsmI 780 GCATTC GAATGC(1/-1) BsoBI 26 CTCGGG C^[CT]CG[GA]G BspDI 634 ATCGAT AT^CGAT BsrBI 640 GAGCGG CCGCTC(-3/-3) BsrDI 791 CATTGC GCAATG(2/0) BstUI 9 CGCG CG^CG BstYI 392 GGATCT [GA]^GATC[CT] CelII 268 GCTAAGC GC^T[ACGT]AGC ClaI 634 ATCGAT AT^CGAT DraII 696 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 821 CCGTGG C^C[GA][CT]GG EaeI 894 TGGCCA [CT]^GGCC[GA] Eco47III 53 AGCGCT AGC^GCT EcoNI 357 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 696 AGGCCCT [GA]G^G[ACGT]CC[CT] HapII 174 CCGG C^CGG HgaI 837 GCGTC GACGC(5/10) HpaII 174 CCGG C^CGG MflI 392 GGATCT [GA]^GATC[CT] MluNI 894 TGGCCA TGG^CCA MscI 894 TGGCCA TGG^CCA MseI 490 TTAA T^TAA MslI 522 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 174 CCGG C^CGG MvnI 9 CGCG CG^CG NciI 174 CCGGG CC^[GC]GG PleI 621 GAGTC GAGTC(4/5) PshAI 645 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 865 CAGCTG CAG^CTG RsaI 311 GTAC GT^AC SacI 604 GAGCTC GAGCT^C SapI 606 GCTCTTC GCTCTTC(1/4) SbfI 555 CCTGCAGG CCTGCA^GG SmlI 406 CTTGAG C^T[CT][GA]AG SphI 517 GCATGC GCATG^C Sse8387I 555 CCTGCAGG CCTGCA^GG Tru9I 490 TTAA T^TAA XhoII 392 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 366 GGATC GGATC(4/5) 392 GGATC AspHI 579 GTGCAC G[AT]GC[AT]^C 604 GAGCTC BfaI 106 CTAG C^TAG 661 CTAG BsgI 616 GTGCAG GTGCAG(16/14) 745 GTGCAG BsiHKAI 579 GTGCAC G[AT]GC[AT]^C 604 GAGCTC BssSI 160 CACGAG CACGAG(-5/-1) 238 CTCGTG Cac8I 517 GCATGC GC[ACGT]^[ACGT]GC 668 GCGTGC DpnII 367 GATC ^GATC 393 GATC EcoT14I 422 CCAAGG C^C[AT][AT]GG 660 CCTAGG EcoT22I 202 ATGCAT ATGCA^T 519 ATGCAT FauI 539 CCCGC CCCGC(4/6) 828 CCCGC HaeII 53 AGCGCT [GA]GCGC^[CT] 873 GGCGCT HphI 724 GGTGA GGTGA(8/7) 219 TCACC MaeI 106 CTAG C^TAG 661 CTAG MboI 367 GATC ^GATC 393 GATC MspA1I 574 CAGCGG C[AC]G^C[GT]G 865 CAGCTG MwoI 412 GCCATTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 867 GCTGGAGGCGC NdeII 367 GATC ^GATC 393 GATC NsiI 202 ATGCAT ATGCA^T 519 ATGCAT NspI 200 ACATGC [GA]CATG^[CT] 517 GCATGC PflMI 69 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 886 CCAGCTCCTGG Sau3AI 367 GATC ^GATC 393 GATC StuI 278 AGGCCT AGG^CCT 402 AGGCCT StyI 422 CCAAGG C^C[AT][AT]GG 660 CCTAGG TaqI 635 TCGA T^CGA 811 TCGA TfiI 149 GAATC G^A[AT]TC 510 GAATC TspRI 625 CACTG CA[GC]TG[ACGT][ACGT]^ 729 CAGTG TthHB8I 635 TCGA T^CGA 811 TCGA Van91I 69 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 886 CCAGCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AlwNI AvaII BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI Ksp632I MaeIII MboII MnlI MvaI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI TseI Tsp45I