(Circular) (Six-base) MAPSORT of: pFN21AE3409.seq Check: 4856 from: 1 to: 5620 pFN21AE3409 5620 bp With 182 enzymes: SgfI * March 28, 2012 12:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 1937 Size: 1026 4594 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 2363 2775 3683 5067 5335 1632 Size: 536 195 412 908 1384 268 1917 Fragments arranged by size: 1917 1384 908 536 412 268 195 AfeI AGC'GCT Cuts at: 2060 2060 Size: 5620 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 2078 Size: 32 5588 AlwNI CAG_nnn'CTG Cuts at: 2080 2554 2809 2897 3363 4935 2080 Size: 474 255 88 466 1572 2765 Fragments arranged by size: 2765 1572 474 466 255 88 ApaLI G'TGCA_C Cuts at: 2584 3677 4833 2584 Size: 1093 1156 3371 Fragments arranged by size: 3371 1156 1093 ApoI r'AATT_y Cuts at: 1493 2929 3155 3307 5416 1493 Size: 1436 226 152 2109 1697 Fragments arranged by size: 2109 1697 1436 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2031 2945 1870 Size: 73 88 914 4545 Fragments arranged by size: 4545 914 88 73 AvrII C'CTAG_G Cuts at: 2665 2665 Size: 5620 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2941 4396 618 Size: 325 217 627 1154 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 217 BanII G_rGCy'C Cuts at: 1391 2543 2605 2613 2939 1391 Size: 1152 62 8 326 4072 Fragments arranged by size: 4072 1152 326 62 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4649 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3811 3845 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 1874 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BfrBI ATG'CAT Cuts at: 2209 2526 2209 Size: 317 5303 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 2274 3037 2274 Size: 763 4857 Bme1580I G_kGCm'C Cuts at: 1790 2588 3681 4837 1790 Size: 798 1093 1156 2573 Fragments arranged by size: 2573 1156 1093 798 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2090 2578 3351 Size: 416 53 83 399 24 673 488 773 Cuts at: 3351 4309 442 Size: 958 1753 Fragments arranged by size: 1753 958 773 673 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2632 2632 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2894 4280 1239 Size: 1655 1386 2579 Fragments arranged by size: 2579 1655 1386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2432 3745 4610 4908 2432 Size: 1313 865 298 3144 Fragments arranged by size: 3144 1313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4283 882 Size: 617 2784 2219 Fragments arranged by size: 2784 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 1178 Size: 30 165 30 675 30 4690 Fragments arranged by size: 4690 675 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2849 2852 1186 Size: 1012 651 3 3954 Fragments arranged by size: 3954 1012 651 3 BseYI C'CCAG_C Cuts at: 2190 2392 2577 2890 4823 5319 2190 Size: 202 185 313 1933 496 2491 Fragments arranged by size: 2491 1933 496 313 202 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2642 2771 1163 Size: 573 906 129 4012 Fragments arranged by size: 4012 906 573 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2588 2613 3681 3766 4837 2588 Size: 25 1068 85 1071 3371 Fragments arranged by size: 3371 1071 1068 85 25 BsmI GAATG_Cn' Cuts at: 2785 3131 3224 2785 Size: 346 93 5181 Fragments arranged by size: 5181 346 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2543 2588 2605 2613 2939 3681 3766 Size: 399 753 45 17 8 326 742 85 Cuts at: 3766 4837 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 753 742 399 326 85 45 17 8 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 844 Size: 2131 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2647 3507 1394 Size: 429 824 860 3507 Fragments arranged by size: 3507 860 824 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2795 4115 4289 65 Size: 1183 1547 1320 174 1396 Fragments arranged by size: 1547 1396 1320 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 2165 2243 3680 4692 2165 Size: 78 1437 1012 3093 Fragments arranged by size: 3093 1437 1012 78 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2397 2950 3317 3702 3719 5171 5257 5269 Size: 425 553 367 385 17 1452 86 12 Cuts at: 5269 5615 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 553 425 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2826 513 Size: 552 939 822 3307 Fragments arranged by size: 3307 939 822 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2195 487 Size: 683 56 969 3912 Fragments arranged by size: 3912 969 683 56 BtsI GCAGTG_nn' Cuts at: 3136 3930 3950 3136 Size: 794 20 4806 Fragments arranged by size: 4806 794 20 ClaI AT'CG_AT Cuts at: 1554 2640 1554 Size: 1086 4534 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2899 3067 3956 Size: 54 1425 50 275 18 1069 168 889 Cuts at: 3956 5585 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1069 889 275 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2222 2618 3550 1353 Size: 719 150 396 932 3423 Fragments arranged by size: 3423 932 719 396 150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4207 4581 4727 387 Size: 1064 138 2618 374 146 1280 Fragments arranged by size: 2618 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2611 2611 Size: 5620 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2363 2775 2894 3683 4280 5067 Size: 393 536 195 412 119 789 597 787 Cuts at: 5067 5335 1239 Size: 268 1524 Fragments arranged by size: 1524 789 787 597 536 412 393 268 195 119 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2366 Size: 5620 EcoO109I rG'GnC_Cy Cuts at: 2702 2702 Size: 5620 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 2062 2882 4767 1678 Size: 384 820 1885 2531 Fragments arranged by size: 2531 1885 820 384 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2388 2645 2677 4261 Size: 32 135 32 784 32 257 32 1584 Cuts at: 4261 4293 4335 4367 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1584 784 257 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4734 4918 1305 Size: 3429 184 2007 Fragments arranged by size: 3429 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2901 10 Size: 54 1425 343 1069 2729 Fragments arranged by size: 2729 1425 1069 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 3578 3937 4096 518 Size: 648 1365 1047 359 159 2042 Fragments arranged by size: 2042 1365 1047 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2581 2872 3036 3713 4861 5106 1606 Size: 975 291 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 975 677 291 245 164 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4129 Fragments arranged by size: 4129 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NsiI A_TGCA'T Cuts at: 2211 2528 2211 Size: 317 5303 NspI r_CATG'y Cuts at: 1132 1229 2209 2526 2978 4523 1132 Size: 97 980 317 452 1545 2229 Fragments arranged by size: 2229 1545 980 452 317 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 2080 2897 1108 Size: 972 817 3831 Fragments arranged by size: 3831 972 817 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2078 2110 3689 3721 5202 5234 2078 Size: 32 1579 32 1481 32 2464 Fragments arranged by size: 2464 1579 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2654 1613 Size: 1041 4579 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PstI C_TGCA'G Cuts at: 830 2405 2565 2886 2972 830 Size: 1575 160 321 86 3478 Fragments arranged by size: 3478 1575 321 160 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 PvuII CAG'CTG Cuts at: 2872 2872 Size: 5620 SacI G_AGCT'C Cuts at: 2613 2613 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SapI GCTCTTCn'nnn_ Cuts at: 2618 2618 Size: 5620 SbfI CC_TGCA'GG Cuts at: 2565 2972 2565 Size: 407 5213 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2401 2561 2882 2968 4103 4784 4975 Size: 219 1356 160 321 86 1135 681 191 Cuts at: 4975 826 Size: 1471 Fragments arranged by size: 1471 1356 1135 681 321 219 191 160 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 3724 4625 4887 820 Size: 197 926 468 1313 901 262 1553 Fragments arranged by size: 1553 1313 926 901 468 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2526 2978 1132 Size: 1394 452 3774 Fragments arranged by size: 3774 1394 452 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 2285 2409 1517 Size: 768 124 4728 Fragments arranged by size: 4728 768 124 StyI C'CwwG_G Cuts at: 513 1065 2004 2427 2665 3059 513 Size: 552 939 423 238 394 3074 Fragments arranged by size: 3074 939 552 423 394 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2381 3647 3664 3817 4002 4447 325 Size: 1255 801 1266 17 153 185 445 1498 Fragments arranged by size: 1498 1266 1255 801 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI