################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccIII AclI AflII AgeI AscI AspI AviII AvrII BclI BfrI BlnI BseAI BsgI BsiWI BsmBI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KspI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 71 enzymes) # ################################### Acc65I 98 GGTACC G^GTACC AccII 64 CGCG CG^CG AcyI 2617 GGCGCC G[GA]^CG[CT]C AflIII 645 ACATGT A^C[GA][CT]GT AhdI 2392 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1454 GTGCAC G^TGCAC AocI 2452 CCTCAGG CC^T[ACGT]AGG Aor51HI 460 AGCGCT AGC^GCT ApaI 3232 GGGCCC GGGCC^C ApaLI 1454 GTGCAC G^TGCAC AseI 2107 ATTAAT AT^TAAT AsnI 2107 ATTAAT AT^TAAT Asp700I 2607 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 98 GGTACC G^GTACC AspEI 2392 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2617 GGCGCC GGCGC^C BbrPI 856 CACGTG CAC^GTG BbvCI 3115 CCTCAGC CCTCAGC(-5/-2) BcgI 2307 GCACATGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 328 GCCCAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2665 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2665 GCTGAGC GC^T[ACGT]AGC BsaAI 856 CACGTG [CT]AC^GT[GA] BsaBI 1578 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2617 GGCGCC G[GA]^CG[CT]C BsaI 1779 GAGACC GGTCTC(1/5) BsiEI 2302 CGGCCG CG[GA][CT]^CG Bsp1407I 546 TGTACA T^GTACA BspLU11I 645 ACATGT A^CATGT BsrFI 2085 ACCGGC [GA]^CCGG[CT] BsrGI 546 TGTACA T^GTACA BstAPI 84 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1191 GGTTACC G^GT[ACGT]ACC BstPI 1191 GGTTACC G^GT[ACGT]ACC BstUI 64 CGCG CG^CG Bsu36I 2452 CCTCAGG CC^T[ACGT]AGG CelII 2665 GCTGAGC GC^T[ACGT]AGC Cfr10I 2085 ACCGGC [GA]^CCGG[CT] EagI 2302 CGGCCG C^GGCCG Eam1105I 2392 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2302 CGGCCG C^GGCCG Eco47III 460 AGCGCT AGC^GCT Eco52I 2302 CGGCCG C^GGCCG Eco81I 2452 CCTCAGG CC^T[ACGT]AGG EcoO65I 1191 GGTTACC G^GT[ACGT]ACC EcoRI 2460 GAATTC G^AATTC EcoT22I 1025 ATGCAT ATGCA^T HgaI 65 GCGTC GACGC(5/10) Hin1I 2617 GGCGCC G[GA]^CG[CT]C KasI 2617 GGCGCC G^GCGCC KpnI 98 GGTACC GGTAC^C MamI 1578 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 350 CAATTG C^AATTG MunI 350 CAATTG C^AATTG MvnI 64 CGCG CG^CG NarI 2617 GGCGCC GG^CGCC NsiI 1025 ATGCAT ATGCA^T NspI 645 ACATGT [GA]CATG^[CT] PflMI 349 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 856 CACGTG CAC^GTG PmlI 856 CACGTG CAC^GTG PshBI 2107 ATTAAT AT^TAAT SfoI 2617 GGCGCC GGC^GCC SmaI 110 CCCGGG CCC^GGG SmlI 809 CTCAAG C^T[CT][GA]AG SspBI 546 TGTACA T^GTACA Van91I 349 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 509 CCACGCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 110 CCCGGG C^CCGGG XmaI 110 CCCGGG C^CCGGG XmnI 2607 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccI 787 GTAGAC GT^[AC][GT]AC 2977 GTAGAC AcsI 1295 GAATTT [GA]^AATT[CT] 2460 GAATTC ApoI 1295 GAATTT [GA]^AATT[CT] 2460 GAATTC BamHI 177 GGATCC G^GATCC 1791 GGATCC BglII 2074 AGATCT A^GATCT 3102 AGATCT BpmI 1465 CTGGAG CTGGAG(16/14) 2713 CTGGAG BsaWI 1324 ACCGGA [AT]^CCGG[AT] 3107 TCCGGT BsmI 2901 GAATGC GAATGC(1/-1) 3200 GAATGC BspMI 441 ACCTGC ACCTGC(4/8) 719 GCAGGT BstXI 2019 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2231 CCAGCTCCTTGG MaeII 857 ACGT A^CGT 3170 ACGT NcoI 966 CCATGG C^CATGG 1724 CCATGG PvuII 58 CAGCTG CAG^CTG 1645 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII