Restriction map for
pFN21AB8028
(Circular) (Six-base) MAPSORT of: pFN21AB8028.seq Check: 1025 from: 1 to: 8017
pFN21AB8028 8017 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2455 2455
Size: 8017
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5827 278
Size: 53 83 186 936 4291 2468
Fragments arranged by size:
4291 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2793 4983 5360 1937
Size: 856 2190 377 4594
Fragments arranged by size:
4594 2190 856 377
Acc65I G'GTAC_C
Cuts at: 2103 5338 2103
Size: 3235 4782
AclI AA'CG_TT
Cuts at: 1563 6144 6517 1563
Size: 4581 373 3063
Fragments arranged by size:
4581 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 3195 4060 4401 6080 7464 7732 1632
Size: 497 1066 865 341 1679 1384 268 1917
Fragments arranged by size:
1917 1679 1384 1066 865 497 341 268
AfeI AGC'GCT
Cuts at: 2467 2467
Size: 8017
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7820
AflIII A'CryG_T
Cuts at: 1203 2650 6916 7893 1203
Size: 1447 4266 977 1327
Fragments arranged by size:
4266 1447 1327 977
AgeI A'CCGG_T
Cuts at: 6848 6848
Size: 8017
AhdI GACnn_n'nnGTC
Cuts at: 4402 6746 4402
Size: 2344 5673
AleI CACnn'nnGTG
Cuts at: 1166 2478 1166
Size: 1312 6705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2060 2423 1184
Size: 876 363 6778
Fragments arranged by size:
6778 876 363
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3001 3033 3001
Size: 32 7985
AlwNI CAG_nnn'CTG
Cuts at: 2095 2491 3521 4979 5760 7332 2095
Size: 396 1030 1458 781 1572 2780
Fragments arranged by size:
2780 1572 1458 1030 781 396
ApaI G_GGCC'C
Cuts at: 5241 5241
Size: 8017
ApaLI G'TGCA_C
Cuts at: 3459 6074 7230 3459
Size: 2615 1156 4246
Fragments arranged by size:
4246 2615 1156
ApoI r'AATT_y
Cuts at: 1493 3300 4465 5326 5552 5704 7813 1493
Size: 1807 1165 861 226 152 2109 1697
Fragments arranged by size:
2109 1807 1697 1165 861 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4113 6571 160
Size: 872 3081 2458 1606
Fragments arranged by size:
3081 2458 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 3063 3429 3530 5004 5342 1870
Size: 73 172 948 366 101 1474 338 4545
Fragments arranged by size:
4545 1474 948 366 338 172 101 73
BamHI G'GATC_C
Cuts at: 2182 3796 5347 5714 2182
Size: 1614 1551 367 4485
Fragments arranged by size:
4485 1614 1551 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2103 4622 4882 5338
Size: 325 217 627 257 59 2519 260 456
Cuts at: 5338 6793 618
Size: 1455 1842
Fragments arranged by size:
2519 1842 1455 627 456 325 260 257
217 59
BanII G_rGCy'C
Cuts at: 1391 2082 2276 2306 4418 4438 5013 5050 5097
Size: 691 194 30 2112 20 575 37 47
Cuts at: 5097 5241 5336 1391
Size: 144 95 4072
Fragments arranged by size:
4072 2112 691 575 194 144 95 47
37 30 20
BbeI G_GCGC'C
Cuts at: 4626 4626
Size: 8017
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 2535 2721 2815 4842 928
Size: 971 218 418 186 94 2027 4103
Fragments arranged by size:
4103 2027 971 418 218 186 94
BbvCI CC'TCA_GC
Cuts at: 5121 5121
Size: 8017
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4301 4335 6208 6242 1903
Size: 34 2364 34 1873 34 3678
Fragments arranged by size:
3678 2364 1873 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 4787 5127 5909 7119 3893
Size: 894 340 782 1210 4791
Fragments arranged by size:
4791 1210 894 782 340
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8017
BfrBI ATG'CAT
Cuts at: 3032 3032
Size: 8017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2339 6628 136
Size: 107 122 71 631 605 667 4289 1525
Fragments arranged by size:
4289 1525 667 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4079 5107 8012 4079
Size: 1028 2905 4084
Fragments arranged by size:
4084 2905 1028
BlpI GC'TnA_GC
Cuts at: 4671 5434 4671
Size: 763 7254
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2661 3463 5241 6078 7234 1790
Size: 259 612 802 1778 837 1156 2573
Fragments arranged by size:
2573 1778 1156 837 802 612 259
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8017
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2146 2271 2426
Size: 416 53 83 399 24 729 125 155
Cuts at: 2426 5748 6706 442
Size: 3322 958 1753
Fragments arranged by size:
3322 1753 958 729 416 399 155 125
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8017
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3491 4739 6677 1239
Size: 2252 1248 1938 2579
Fragments arranged by size:
2579 2252 1938 1248
Bpu10I CC'TnA_GC
Cuts at: 2808 3362 5096 5121 2808
Size: 554 1734 25 5704
Fragments arranged by size:
5704 1734 554 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2799 6142 7007 7305 2799
Size: 3343 865 298 3511
Fragments arranged by size:
3511 3343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3778 6680 882
Size: 617 2279 2902 2219
Fragments arranged by size:
2902 2279 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2863 5808 493
Size: 711 1659 2945 2702
Fragments arranged by size:
2945 2702 1659 711
BsaBI GATnn'nnATC
Cuts at: 3587 8011 3587
Size: 4424 3593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4623 5824 6206 6841
Size: 53 83 186 936 3090 1201 382 635
Cuts at: 6841 275
Size: 1451
Fragments arranged by size:
3090 1451 1201 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3329 5112 6450 6848 7122 7269 7680 3329
Size: 1783 1338 398 274 147 411 3666
Fragments arranged by size:
3666 1783 1338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2410 2440 4804 4834 4902
Size: 30 165 30 1007 30 2364 30 68
Cuts at: 4902 4928 4932 4958 1178
Size: 26 4 26 4237
Fragments arranged by size:
4237 2364 1007 165 68 30 30 30
30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2431 2846 3787 4592 1186
Size: 1245 415 941 805 4611
Fragments arranged by size:
4611 1245 941 805 415
BseYI C'CCAG_C
Cuts at: 2085 2457 3076 5148 5193 7220 7716 2085
Size: 372 619 2072 45 2027 496 2386
Fragments arranged by size:
2386 2072 2027 619 496 372 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7444
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4310 5467 6228 7256 1540
Size: 275 186 2309 1157 761 1028 2301
Fragments arranged by size:
2309 2301 1157 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2856 3371 3463 4418 4438 5097 6078 6163
Size: 774 515 92 955 20 659 981 85
Cuts at: 6163 7234 2082
Size: 1071 2865
Fragments arranged by size:
2865 1071 981 955 774 659 515 92
85 20
BsmI GAATG_Cn'
Cuts at: 4912 5211 5528 5621 4912
Size: 299 317 93 7308
Fragments arranged by size:
7308 317 299 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2082 2276 2306 2661 2856 3371
Size: 399 259 33 194 30 355 195 515
Cuts at: 3371 3463 4418 4438 5013 5050 5097 5241 5336
Size: 92 955 20 575 37 47 144 95
Cuts at: 5336 6078 6163 7234 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 955 742 575 515 399 355
259 195 194 144 95 92 85 47
37 33 30 20
BspEI T'CCGG_A
Cuts at: 7680 7680
Size: 8017
BspHI T'CATG_A
Cuts at: 5906 7636 5906
Size: 1730 6287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2455 2715 5372 844
Size: 1611 260 2657 3489
Fragments arranged by size:
3489 2657 1611 260
BsrBI CCG'CTC
Cuts at: 1394 1823 5904 1394
Size: 429 4081 3507
Fragments arranged by size:
4081 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2841 3399 4342 6512 6686 65
Size: 1183 1593 558 943 2170 174 1396
Fragments arranged by size:
2170 1593 1396 1183 943 558 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4090 6661 6848 1700
Size: 110 2280 2571 187 2869
Fragments arranged by size:
2869 2571 2280 187 110
BsrGI T'GTAC_A
Cuts at: 96 2551 96
Size: 2455 5562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8017
BssSI C'ACGA_G
Cuts at: 6077 7089 6077
Size: 1012 7005
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 794
Size: 1301 6716
BstBI TT'CG_AA
Cuts at: 6821 6821
Size: 8017
BstEII G'GTnAC_C
Cuts at: 1184 3196 1184
Size: 2012 6005
BstXI CCAn_nnnn'nTGG
Cuts at: 4031 4243 4031
Size: 212 7805
BstYI r'GATC_y
Cuts at: 1972 2182 2785 3498 3796 4079 4588 4975 5107
Size: 210 603 713 298 283 509 387 132
Cuts at: 5107 5347 5714 6099 6116 7568 7654 7666 8012
Size: 240 367 385 17 1452 86 12 346
Cuts at: 8012 1972
Size: 1977
Fragments arranged by size:
1977 1452 713 603 509 387 385 367
346 298 283 240 210 132 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 4458 4458
Size: 8017
BtgI C'CryG_G
Cuts at: 513 1065 2971 3729 5242 513
Size: 552 1906 758 1513 3288
Fragments arranged by size:
3288 1906 1513 758 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3375 487
Size: 683 56 2149 5129
Fragments arranged by size:
5129 2149 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 2431 4269 5533 6327 6347 2405
Size: 26 1838 1264 794 20 4075
Fragments arranged by size:
4075 1838 1264 794 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2595 2630 529
Size: 35 2031 35 5916
Fragments arranged by size:
5916 2031 35 35
DraI TTT'AAA
Cuts at: 5321 5321
Size: 8017
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7024 809
Size: 507 5708 1802
Fragments arranged by size:
5708 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 2340 2482
Size: 54 1425 50 275 18 498 12 142
Cuts at: 2482 3277 3354 4092 4266 4307 4426 4747 5464
Size: 795 77 738 174 41 119 321 717
Cuts at: 5464 6353 7982 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 795 738 717 498 321
275 174 142 119 77 54 50 43
41 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 4307 5464 1537
Size: 275 2495 1157 4090
Fragments arranged by size:
4090 2495 1157 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2702 3214 3339 4124 4707 4728 4843 5947
Size: 1349 512 125 785 583 21 115 1104
Cuts at: 5947 1353
Size: 3423
Fragments arranged by size:
3423 1349 1104 785 583 512 125 115
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2816 5169 6604 6978 7124 387
Size: 1064 138 1227 2353 1435 374 146 1280
Fragments arranged by size:
2353 1435 1280 1227 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2080 4416 4436 5095 2080
Size: 2336 20 659 5002
Fragments arranged by size:
5002 2336 659 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 3195 3491 4060 4401 4739 6080
Size: 393 497 1066 296 569 341 338 1341
Cuts at: 6080 6677 7464 7732 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1341 1066 787 597 569 497 393
341 338 296 268
EcoNI CCTnn'n_nnAGG
Cuts at: 4297 4957 5067 4297
Size: 660 110 7247
Fragments arranged by size:
7247 660 110
EcoO109I rG'GnC_Cy
Cuts at: 2126 3170 4302 4967 5159 5237 5238 2126
Size: 1044 1132 665 192 78 1 4905
Fragments arranged by size:
4905 1132 1044 665 192 78 1
EcoRI G'AATT_C
Cuts at: 4465 5326 5704 4465
Size: 861 378 6778
Fragments arranged by size:
6778 861 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3847 4633 3847
Size: 786 7231
FspI TGC'GCA
Cuts at: 6523 6523
Size: 8017
HaeII r_GCGC'y
Cuts at: 1678 2096 2469 3615 4626 7164 1678
Size: 418 373 1146 1011 2538 2531
Fragments arranged by size:
2538 2531 1146 1011 418 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2685 2717 2983 3015 3374
Size: 32 135 32 1113 32 266 32 359
Cuts at: 3374 3406 3738 3770 3993 4025 4388 4400 4420
Size: 32 332 32 223 32 363 12 20
Cuts at: 4420 4432 6658 6690 6732 6764 1373
Size: 12 2226 32 42 32 2626
Fragments arranged by size:
2626 2226 1113 363 359 332 266 223
135 42 32 32 32 32 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 5361 5607 669
Size: 988 281 3423 246 3079
Fragments arranged by size:
3423 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8017
HpaI GTT'AAC
Cuts at: 5607 5607
Size: 8017
KasI G'GCGC_C
Cuts at: 4622 4622
Size: 8017
KpnI G_GTAC'C
Cuts at: 2107 5342 2107
Size: 3235 4782
MfeI C'AATT_G
Cuts at: 2355 5616 2355
Size: 3261 4756
MluI A'CGCG_T
Cuts at: 7893 7893
Size: 8017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3714 4004 4238 7131 7315 1305
Size: 2409 290 234 2893 184 2007
Fragments arranged by size:
2893 2409 2007 290 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2484 3356 4428 10
Size: 54 1425 343 652 872 1072 3599
Fragments arranged by size:
3599 1425 1072 872 652 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2042 2478 2754 5975 6334 6493
Size: 648 843 33 436 276 3221 359 159
Cuts at: 6493 518
Size: 2042
Fragments arranged by size:
3221 2042 843 648 436 359 276 159
33
MspA1I CmG'CkG
Cuts at: 1606 2065 2101 3652 5433 6110 7258 7503 1606
Size: 459 36 1551 1781 677 1148 245 2120
Fragments arranged by size:
2120 1781 1551 1148 677 459 245 36
NarI GG'CG_CC
Cuts at: 4623 4623
Size: 8017
NcoI C'CATG_G
Cuts at: 513 1065 2971 3729 513
Size: 552 1906 758 4801
Fragments arranged by size:
4801 1906 758 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8017
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8017
NotI GC'GGCC_GC
Cuts at: 5464 5464
Size: 8017
NsiI A_TGCA'T
Cuts at: 3034 3034
Size: 8017
NspI r_CATG'y
Cuts at: 1132 1229 2654 5375 6920 1132
Size: 97 1425 2721 1545 2229
Fragments arranged by size:
2721 2229 1545 1425 97
PasI CC'CwG_GG
Cuts at: 1348 4959 1348
Size: 3611 4406
PciI A'CATG_T
Cuts at: 2650 6916 2650
Size: 4266 3751
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 1108
Size: 1252 6765
PfoI T'CCnGG_A
Cuts at: 2178 2178
Size: 8017
PmeI GTTT'AAAC
Cuts at: 5321 5321
Size: 8017
PmlI CAC'GTG
Cuts at: 2863 2863
Size: 8017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6086 6118 7599 7631 6086
Size: 32 1481 32 6472
Fragments arranged by size:
6472 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2126 4967 5159 2126
Size: 2841 192 4984
Fragments arranged by size:
4984 2841 192
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8017
PsiI TTA'TAA
Cuts at: 5587 5587
Size: 8017
PspOMI G'GGCC_C
Cuts at: 5237 5237
Size: 8017
PstI C_TGCA'G
Cuts at: 830 2100 2293 3805 4257 4264 5311 5369 830
Size: 1270 193 1512 452 7 1047 58 3478
Fragments arranged by size:
3478 1512 1270 1047 452 193 58 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8017
PvuII CAG'CTG
Cuts at: 2065 3652 2065
Size: 1587 6430
SacI G_AGCT'C
Cuts at: 2082 4418 4438 5097 2082
Size: 2336 20 659 5002
Fragments arranged by size:
5002 2336 659 20
SalI G'TCGA_C
Cuts at: 1936 5359 1936
Size: 3423 4594
SapI GCTCTTCn'nnn_
Cuts at: 3339 4124 4707 4728 4843 3339
Size: 785 583 21 115 6513
Fragments arranged by size:
6513 785 583 115 21
SbfI CC_TGCA'GG
Cuts at: 5369 5369
Size: 8017
ScaI AGT'ACT
Cuts at: 6265 7977 6265
Size: 1712 6305
SfcI C'TryA_G
Cuts at: 826 1045 2096 2289 3801 4253 4260 4980 5307
Size: 219 1051 193 1512 452 7 720 327
Cuts at: 5307 5365 6500 7181 7372 826
Size: 58 1135 681 191 1471
Fragments arranged by size:
1512 1471 1135 1051 720 681 452 327
219 193 191 58 7
SfoI GGC'GCC
Cuts at: 4624 4624
Size: 8017
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8017
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8017
SmaI CCC'GGG
Cuts at: 1872 2117 5344 1872
Size: 245 3227 4545
Fragments arranged by size:
4545 3227 245
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2814 6121 7022 7284 820
Size: 197 926 871 3307 901 262 1553
Fragments arranged by size:
3307 1553 926 901 871 262 197
SnaBI TAC'GTA
Cuts at: 493 5808 493
Size: 5315 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8017
SphI G_CATG'C
Cuts at: 1132 5375 1132
Size: 4243 3774
SspI AAT'ATT
Cuts at: 5 52 5941 5
Size: 47 5889 2081
Fragments arranged by size:
5889 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8017
StyI C'CwwG_G
Cuts at: 513 1065 2971 3071 3729 4242 4429 5456 513
Size: 552 1906 100 658 513 187 1027 3074
Fragments arranged by size:
3074 1906 1027 658 552 513 187 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2128 4870 6044 6061 6214 6399
Size: 1255 519 29 2742 1174 17 153 185
Cuts at: 6399 6844 325
Size: 445 1498
Fragments arranged by size:
2742 1498 1255 1174 519 445 185 153
29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2551 6263 7975
Size: 274 80 33 51 1443 574 3712 1712
Cuts at: 7975 96
Size: 138
Fragments arranged by size:
3712 1712 1443 574 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2410 2442 3892 3924 4902 4934 7977 8009 2410
Size: 32 1450 32 978 32 3043 32 2418
Fragments arranged by size:
3043 2418 1450 978 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8017
XbaI T'CTAG_A
Cuts at: 5353 5353
Size: 8017
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2521 2521
Size: 8017
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8017
XmaI C'CCGG_G
Cuts at: 1870 2115 5342 1870
Size: 245 3227 4545
Fragments arranged by size:
4545 3227 245
XmnI GAAnn'nnTTC
Cuts at: 4616 6146 4616
Size: 1530 6487
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5825 276
Size: 53 83 186 936 4291 2468
Fragments arranged by size:
4291 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI