(Linear) (Six-base) MAPSORT of: KIBB8028.seq Check: 768 from: 1 to: 3312 KIBB8028.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 450 3312 Size: 450 2862 AccI GT'mk_AC Cuts at: 0 788 2978 3312 Size: 788 2190 334 Fragments arranged by size: 2190 788 334 Acc65I G'GTAC_C Cuts at: 0 98 3312 Size: 98 3214 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 1190 2055 2396 3312 Size: 124 1066 865 341 916 Fragments arranged by size: 1066 916 865 341 124 AfeI AGC'GCT Cuts at: 0 462 3312 Size: 462 2850 AflIII A'CryG_T Cuts at: 0 645 3312 Size: 645 2667 AhdI GACnn_n'nnGTC Cuts at: 0 2397 3312 Size: 2397 915 AleI CACnn'nnGTG Cuts at: 0 473 3312 Size: 473 2839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 55 418 3312 Size: 55 363 2894 Fragments arranged by size: 2894 363 55 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 996 1028 3312 Size: 996 32 2284 Fragments arranged by size: 2284 996 32 AlwNI CAG_nnn'CTG Cuts at: 0 90 486 1516 2974 3312 Size: 90 396 1030 1458 338 Fragments arranged by size: 1458 1030 396 338 90 ApaI G_GGCC'C Cuts at: 0 3236 3312 Size: 3236 76 ApaLI G'TGCA_C Cuts at: 0 1454 3312 Size: 1454 1858 ApoI r'AATT_y Cuts at: 0 1295 2460 3312 Size: 1295 1165 852 Fragments arranged by size: 1295 1165 852 AseI AT'TA_AT Cuts at: 0 2108 3312 Size: 2108 1204 AvaI C'yCGr_G Cuts at: 0 110 1058 1424 1525 2999 3312 Size: 110 948 366 101 1474 313 Fragments arranged by size: 1474 948 366 313 110 101 BamHI G'GATC_C Cuts at: 0 177 1791 3312 Size: 177 1614 1521 Fragments arranged by size: 1614 1521 177 BanI G'GyrC_C Cuts at: 0 39 98 2617 2877 3312 Size: 39 59 2519 260 435 Fragments arranged by size: 2519 435 260 59 39 BanII G_rGCy'C Cuts at: 0 77 271 301 2413 2433 3008 3045 3092 Size: 77 194 30 2112 20 575 37 47 Cuts at: 3092 3236 3312 Size: 144 76 Fragments arranged by size: 2112 575 194 144 77 76 47 37 30 20 BbeI G_GCGC'C Cuts at: 0 2621 3312 Size: 2621 691 BbsI GAAGACnn'nnnn_ Cuts at: 0 112 530 716 810 2837 3312 Size: 112 418 186 94 2027 475 Fragments arranged by size: 2027 475 418 186 112 94 BbvCI CC'TCA_GC Cuts at: 0 3116 3312 Size: 3116 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2296 2330 3312 Size: 2296 34 982 Fragments arranged by size: 2296 982 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1888 2782 3122 3312 Size: 1888 894 340 190 Fragments arranged by size: 1888 894 340 190 BfrBI ATG'CAT Cuts at: 0 1027 3312 Size: 1027 2285 BglI GCCn_nnn'nGGC Cuts at: 0 334 3312 Size: 334 2978 BglII A'GATC_T Cuts at: 0 2074 3102 3312 Size: 2074 1028 210 Fragments arranged by size: 2074 1028 210 BlpI GC'TnA_GC Cuts at: 0 2666 3312 Size: 2666 646 Bme1580I G_kGCm'C Cuts at: 0 44 656 1458 3236 3312 Size: 44 612 802 1778 76 Fragments arranged by size: 1778 802 612 76 44 BmrI ACTGGGnnnn_n' Cuts at: 0 141 266 421 3312 Size: 141 125 155 2891 Fragments arranged by size: 2891 155 141 125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1486 2734 3312 Size: 1486 1248 578 Fragments arranged by size: 1486 1248 578 Bpu10I CC'TnA_GC Cuts at: 0 803 1357 3091 3116 3312 Size: 803 554 1734 25 196 Fragments arranged by size: 1734 803 554 196 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 794 3312 Size: 794 2518 BsaI GGTCTCn'nnnn_ Cuts at: 0 1773 3312 Size: 1773 1539 BsaAI yAC'GTr Cuts at: 0 858 3312 Size: 858 2454 BsaBI GATnn'nnATC Cuts at: 0 1582 3312 Size: 1582 1730 BsaHI Gr'CG_yC Cuts at: 0 2618 3312 Size: 2618 694 BsaWI w'CCGG_w Cuts at: 0 1324 3107 3312 Size: 1324 1783 205 Fragments arranged by size: 1783 1324 205 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 405 435 2799 2829 2897 2923 2927 2953 Size: 405 30 2364 30 68 26 4 26 Cuts at: 2953 3312 Size: 359 Fragments arranged by size: 2364 405 359 68 30 30 26 26 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 426 841 1782 2587 3312 Size: 426 415 941 805 725 Fragments arranged by size: 941 805 725 426 415 BseYI C'CCAG_C Cuts at: 0 80 452 1071 3143 3188 3312 Size: 80 372 619 2072 45 124 Fragments arranged by size: 2072 619 372 124 80 45 BsiEI CG_ry'CG Cuts at: 0 2305 3312 Size: 2305 1007 BsiHKAI G_wGCw'C Cuts at: 0 77 851 1366 1458 2413 2433 3092 3312 Size: 77 774 515 92 955 20 659 220 Fragments arranged by size: 955 774 659 515 220 92 77 20 BsmI GAATG_Cn' Cuts at: 0 2907 3206 3312 Size: 2907 299 106 Fragments arranged by size: 2907 299 106 Bsp1286I G_dGCh'C Cuts at: 0 44 77 271 301 656 851 1366 1458 Size: 44 33 194 30 355 195 515 92 Cuts at: 1458 2413 2433 3008 3045 3092 3236 3312 Size: 955 20 575 37 47 144 76 Fragments arranged by size: 955 575 515 355 195 194 144 92 76 47 44 37 33 30 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 450 710 3312 Size: 450 260 2602 Fragments arranged by size: 2602 450 260 BsrDI GCAATG_nn' Cuts at: 0 836 1394 2337 3312 Size: 836 558 943 975 Fragments arranged by size: 975 943 836 558 BsrFI r'CCGG_y Cuts at: 0 2085 3312 Size: 2085 1227 BsrGI T'GTAC_A Cuts at: 0 546 3312 Size: 546 2766 BstAPI GCAn_nnn'nTGC Cuts at: 0 90 3312 Size: 90 3222 BstEII G'GTnAC_C Cuts at: 0 1191 3312 Size: 1191 2121 BstXI CCAn_nnnn'nTGG Cuts at: 0 2026 2238 3312 Size: 2026 212 1074 Fragments arranged by size: 2026 1074 212 BstYI r'GATC_y Cuts at: 0 177 780 1493 1791 2074 2583 2970 3102 Size: 177 603 713 298 283 509 387 132 Cuts at: 3102 3312 Size: 210 Fragments arranged by size: 713 603 509 387 298 283 210 177 132 Bsu36I CC'TnA_GG Cuts at: 0 2453 3312 Size: 2453 859 BtgI C'CryG_G Cuts at: 0 966 1724 3237 3312 Size: 966 758 1513 75 Fragments arranged by size: 1513 966 758 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1370 3312 Size: 1370 1942 BtsI GCAGTG_nn' Cuts at: 0 400 426 2264 3312 Size: 400 26 1838 1048 Fragments arranged by size: 1838 1048 400 26 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 590 625 3312 Size: 590 35 2687 Fragments arranged by size: 2687 590 35 EaeI y'GGCC_r Cuts at: 0 323 335 477 1272 1349 2087 2261 2302 Size: 323 12 142 795 77 738 174 41 Cuts at: 2302 2421 2742 3312 Size: 119 321 570 Fragments arranged by size: 795 738 570 323 321 174 142 119 77 41 12 EagI C'GGCC_G Cuts at: 0 2302 3312 Size: 2302 1010 EarI CTCTTCn'nnn_ Cuts at: 0 697 1209 1334 2119 2702 2723 2838 3312 Size: 697 512 125 785 583 21 115 474 Fragments arranged by size: 785 697 583 512 474 125 115 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 811 3164 3312 Size: 811 2353 148 Fragments arranged by size: 2353 811 148 EcoICRI GAG'CTC Cuts at: 0 75 2411 2431 3090 3312 Size: 75 2336 20 659 222 Fragments arranged by size: 2336 659 222 75 20 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 1190 1486 2055 2396 2734 3312 Size: 124 1066 296 569 341 338 578 Fragments arranged by size: 1066 578 569 341 338 296 124 EcoNI CCTnn'n_nnAGG Cuts at: 0 2292 2952 3062 3312 Size: 2292 660 110 250 Fragments arranged by size: 2292 660 250 110 EcoO109I rG'GnC_Cy Cuts at: 0 121 1165 2297 2962 3154 3232 3233 3312 Size: 121 1044 1132 665 192 78 1 79 Fragments arranged by size: 1132 1044 665 192 121 79 78 1 EcoRI G'AATT_C Cuts at: 0 2460 3312 Size: 2460 852 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1842 2628 3312 Size: 1842 786 684 Fragments arranged by size: 1842 786 684 HaeII r_GCGC'y Cuts at: 0 91 464 1610 2621 3312 Size: 91 373 1146 1011 691 Fragments arranged by size: 1146 1011 691 373 91 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 680 712 978 1010 1369 1401 1733 1765 Size: 680 32 266 32 359 32 332 32 Cuts at: 1765 1988 2020 2383 2395 2415 2427 3312 Size: 223 32 363 12 20 12 885 Fragments arranged by size: 885 680 363 359 332 266 223 32 32 32 32 32 20 12 12 KasI G'GCGC_C Cuts at: 0 2617 3312 Size: 2617 695 KpnI G_GTAC'C Cuts at: 0 102 3312 Size: 102 3210 MfeI C'AATT_G Cuts at: 0 350 3312 Size: 350 2962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1709 1999 2233 3312 Size: 1709 290 234 1079 Fragments arranged by size: 1709 1079 290 234 MscI TGG'CCA Cuts at: 0 479 1351 2423 3312 Size: 479 872 1072 889 Fragments arranged by size: 1072 889 872 479 MslI CAynn'nnrTG Cuts at: 0 37 473 749 3312 Size: 37 436 276 2563 Fragments arranged by size: 2563 436 276 37 MspA1I CmG'CkG Cuts at: 0 60 96 1647 3312 Size: 60 36 1551 1665 Fragments arranged by size: 1665 1551 60 36 NarI GG'CG_CC Cuts at: 0 2618 3312 Size: 2618 694 NcoI C'CATG_G Cuts at: 0 966 1724 3312 Size: 966 758 1588 Fragments arranged by size: 1588 966 758 NsiI A_TGCA'T Cuts at: 0 1029 3312 Size: 1029 2283 NspI r_CATG'y Cuts at: 0 649 3312 Size: 649 2663 PasI CC'CwG_GG Cuts at: 0 2954 3312 Size: 2954 358 PciI A'CATG_T Cuts at: 0 645 3312 Size: 645 2667 PflMI CCAn_nnn'nTGG Cuts at: 0 355 3312 Size: 355 2957 PfoI T'CCnGG_A Cuts at: 0 173 3312 Size: 173 3139 PmlI CAC'GTG Cuts at: 0 858 3312 Size: 858 2454 PpuMI rG'GwC_Cy Cuts at: 0 121 2962 3154 3312 Size: 121 2841 192 158 Fragments arranged by size: 2841 192 158 121 PspOMI G'GGCC_C Cuts at: 0 3232 3312 Size: 3232 80 PstI C_TGCA'G Cuts at: 0 95 288 1800 2252 2259 3306 3312 Size: 95 193 1512 452 7 1047 6 Fragments arranged by size: 1512 1047 452 193 95 7 6 PvuII CAG'CTG Cuts at: 0 60 1647 3312 Size: 60 1587 1665 Fragments arranged by size: 1665 1587 60 SacI G_AGCT'C Cuts at: 0 77 2413 2433 3092 3312 Size: 77 2336 20 659 220 Fragments arranged by size: 2336 659 220 77 20 SapI GCTCTTCn'nnn_ Cuts at: 0 1334 2119 2702 2723 2838 3312 Size: 1334 785 583 21 115 474 Fragments arranged by size: 1334 785 583 474 115 21 SfcI C'TryA_G Cuts at: 0 91 284 1796 2248 2255 2975 3302 3312 Size: 91 193 1512 452 7 720 327 10 Fragments arranged by size: 1512 720 452 327 193 91 10 7 SfoI GGC'GCC Cuts at: 0 2619 3312 Size: 2619 693 SmaI CCC'GGG Cuts at: 0 112 3312 Size: 112 3200 SmlI C'TyrA_G Cuts at: 0 809 3312 Size: 809 2503 StyI C'CwwG_G Cuts at: 0 966 1066 1724 2237 2424 3312 Size: 966 100 658 513 187 888 Fragments arranged by size: 966 888 658 513 187 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 94 123 2865 3312 Size: 94 29 2742 447 Fragments arranged by size: 2742 447 94 29 TatI w'GTAC_w Cuts at: 0 546 3312 Size: 546 2766 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 405 437 1887 1919 2897 2929 3312 Size: 405 32 1450 32 978 32 383 Fragments arranged by size: 1450 978 405 383 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 516 3312 Size: 516 2796 XmaI C'CCGG_G Cuts at: 0 110 3312 Size: 110 3202 XmnI GAAnn'nnTTC Cuts at: 0 2611 3312 Size: 2611 701 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI KpnI MfeI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AgeI AscI AsiSI AvrII BaeI BclI BmgBI BmtI BplI BsgI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstBI BstZ17I ClaI DraI DraIII DrdI EcoRV FseI FspI FspAI HincII HindIII HpaI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI