################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaII HphI KasI KpnI KspI MaeI MaeII MboI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 33 GCGG CCGC(-3/-1) AfaI 285 GTAC GT^AC AflIII 150 ACATGT A^C[GA][CT]GT AhdI 109 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 109 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 28 CCTCAGC CCTCAGC(-5/-2) BbvI 255 GCAGC GCAGC(8/12) BglI 329 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 119 CTCCAG CTGGAG(16/14) BsaBI 49 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 157 GAGACC GGTCTC(1/5) BsmI 41 GCATTC GAATGC(1/-1) Bsp1407I 284 TGTACA T^GTACA BspLU11I 150 ACATGT A^CATGT BsrGI 284 TGTACA T^GTACA BstF5I 414 CATCC GGATG(2/0) BstXI 185 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 29 CTCAG C^T[ACGT]AG Eam1105I 109 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 316 CTCTTC CTCTTC(1/4) EcoRV 49 GATATC GAT^ATC FauI 33 GCGGG CCCGC(4/6) Fnu4HI 255 GCAGC GC^[ACGT]GC FokI 414 CATCC GGATG(9/13) HaeIII 196 GGCC GG^CC HgaI 84 GACGC GACGC(5/10) HincII 459 GTTAAC GT[CT]^[GA]AC HindII 459 GTTAAC GT[CT]^[GA]AC HpaI 459 GTTAAC GTT^AAC ItaI 255 GCAGC GC^[ACGT]GC Ksp632I 316 CTCTTC CTCTTC(1/4) MaeIII 124 GTCAC ^GT[ACGT]AC MamI 49 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspI 150 ACATGT [GA]CATG^[CT] PflMI 423 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 253 CTGCAG CTGCA^G PvuII 256 CAGCTG CAG^CTG RsaI 285 GTAC GT^AC SfcI 253 CTGCAG C^T[GA][CT]AG SmlI 163 CTTGAG C^T[CT][GA]AG SspBI 284 TGTACA T^GTACA TaqI 353 TCGA T^CGA TseI 255 GCAGC G^C[AT]GC Tsp45I 124 GTCAC ^GT[GC]AC Tsp509I 18 AATT ^AATT TspRI 251 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 353 TCGA T^CGA Van91I 423 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 324 CCACAGCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 257 AGCT AG^CT 391 AGCT AvaI 99 CCCGAG C^[CT]CG[GA]G 295 CCCGAG BsmAI 157 GAGAC GTCTC(1/5) 117 GTCTC BsmFI 239 GGGAC GGGAC(10/14) 97 GTCCC BsoBI 99 CCCGAG C^[CT]CG[GA]G 295 CCCGAG BsrI 429 ACTGG ACTGG(1/-1) 121 CCAGT BssKI 192 CCTGG ^CC[ACGT]GG 370 CCTGG BstNI 192 CCTGG CC^[AT]GG 370 CCTGG EcoRII 192 CCTGG ^CC[AT]GG 370 CCTGG HinfI 206 GACTC G^A[ACGT]TC 355 GAGTC MboII 72 TCTTC GAAGA(8/7) 317 TCTTC MseI 136 TTAA T^TAA 460 TTAA MspA1I 31 CAGCGG C[AC]G^C[GT]G 256 CAGCTG MvaI 192 CCTGG CC^[AT]GG 370 CCTGG MwoI 246 GCCCACACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 329 GCCAGAGTGGC NlaIII 143 CATG CATG^ 151 CATG NlaIV 179 GGAACC GG[ACGT]^[ACGT]CC 239 GGGACC PleI 206 GACTC GAGTC(4/5) 355 GAGTC ScrFI 192 CCTGG CC^[ACGT]GG 370 CCTGG Tru9I 136 TTAA T^TAA 460 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AvaII BsaJI BsiYI BslI Cfr13I MnlI Sau96I