################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AccI AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AvrII BalI BamHI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI MaeI MamI MfeI MluI MluNI MscI MunI NcoI NdeI NheI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 1189 GACGTC GACGT^C Acc65I 526 GGTACC G^GTACC AccIII 301 TCCGGA T^CCGGA AcsI 85 AAATTC [GA]^AATT[CT] Alw44I 768 GTGCAC G^TGCAC ApaLI 768 GTGCAC G^TGCAC ApoI 85 AAATTC [GA]^AATT[CT] Asp700I 1713 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 526 GGTACC G^GTACC AviII 2402 TGCGCA TGC^GCA BbrPI 562 CACGTG CAC^GTG BsaAI 562 CACGTG [CT]AC^GT[GA] BseAI 301 TCCGGA T^CCGGA BsgI 508 CTGCAC GTGCAG(16/14) BspEI 301 TCCGGA T^CCGGA BsrDI 189 CATTGC GCAATG(2/0) BssHII 735 GCGCGC G^CGCGC BstYI 590 GGATCT [GA]^GATC[CT] DraIII 511 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG FspI 2402 TGCGCA TGC^GCA KpnI 526 GGTACC GGTAC^C KspI 1505 CCGCGG CCGC^GG MflI 590 GGATCT [GA]^GATC[CT] MroI 301 TCCGGA T^CCGGA MslI 9 CACAAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 2867 ACATGC [GA]CATG^[CT] PmaCI 562 CACGTG CAC^GTG PmlI 562 CACGTG CAC^GTG PshAI 514 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 2785 CAGCTG CAG^CTG SacII 1505 CCGCGG CCGC^GG SapI 823 GAAGAGC GCTCTTC(1/4) StuI 48 AGGCCT AGG^CCT XhoII 590 GGATCT [GA]^GATC[CT] XmnI 1713 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 590 GGATC GGATC(4/5) 2477 GGATC AlwNI 614 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 2441 CAGGGCCTG BbsI 1186 GAAGAC GAAGAC(2/6) 2533 GAAGAC BlpI 670 GCTAAGC GC^T[ACGT]AGC 2265 GCTCAGC Bpu1102I 670 GCTAAGC GC^T[ACGT]AGC 2265 GCTCAGC BpuAI 1186 GAAGAC GAAGAC(2/6) 2533 GAAGAC BsaI 540 GGTCTC GGTCTC(1/5) 1852 GGTCTC BssSI 1986 CACGAG CACGAG(-5/-1) 1731 CTCGTG CelII 670 GCTAAGC GC^T[ACGT]AGC 2265 GCTCAGC DrdI 571 GACAGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2553 GACCCTGAAGTC EcoNI 51 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 412 CCTCCTCCAGG HindIII 28 AAGCTT A^AGCTT 2855 AAGCTT MaeIII 2485 GTCAC ^GT[ACGT]AC 2864 GTGAC NlaIII 456 CATG CATG^ 2868 CATG SacI 459 GAGCTC GAGCT^C 1018 GAGCTC SfaNI 448 GATGC GCATC(5/9) 2781 GCATC SfiI 1355 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1932 GGCCAGCCCGGCC SmaI 982 CCCGGG CCC^GGG 2349 CCCGGG SmlI 996 CTCAAG C^T[CT][GA]AG 2852 CTCAAG Tsp45I 2485 GTCAC ^GT[GC]AC 2864 GTGAC Tsp509I 86 AATT ^AATT 1434 AATT XcmI 8 CCACAAGGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1043 CCACTCTCCGCGTGG XmaCI 982 CCCGGG C^CCGGG 2349 CCCGGG XmaI 982 CCCGGG C^CCGGG 2349 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AocI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIV NotI NruI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfcI SfoI SgrAI StyI TaqI TfiI Tru9I TseI TspRI TthHB8I