(Circular) (Six-base) MAPSORT of: pFN21AE2355.seq Check: 5918 from: 1 to: 7591 pFN21AE2355 7591 bp With 182 enzymes: SgfI * June 21, 2011 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3198 5401 278 Size: 53 83 186 936 1662 2203 2468 Fragments arranged by size: 2468 2203 1662 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4934 1937 Size: 2997 4594 Acc65I G'GTAC_C Cuts at: 2531 4912 2531 Size: 2381 5210 AclI AA'CG_TT Cuts at: 1563 5718 6091 1563 Size: 4155 373 3063 Fragments arranged by size: 4155 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2614 4583 4814 5654 7038 7306 1632 Size: 982 1969 231 840 1384 268 1917 Fragments arranged by size: 1969 1917 1384 982 840 268 231 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7394 AflIII A'CryG_T Cuts at: 1203 6490 7467 1203 Size: 5287 977 1327 Fragments arranged by size: 5287 1327 977 AgeI A'CCGG_T Cuts at: 6422 6422 Size: 7591 AhdI GACnn_n'nnGTC Cuts at: 6320 6320 Size: 7591 AleI CACnn'nnGTG Cuts at: 1166 2018 1166 Size: 852 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7591 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3291 3323 3291 Size: 32 7559 AlwNI CAG_nnn'CTG Cuts at: 2624 4451 5334 6906 2624 Size: 1827 883 1572 3309 Fragments arranged by size: 3309 1827 1572 883 ApaI G_GGCC'C Cuts at: 2275 2920 3086 3363 3530 4228 2275 Size: 645 166 277 167 698 5638 Fragments arranged by size: 5638 698 645 277 167 166 ApaLI G'TGCA_C Cuts at: 2773 5648 6804 2773 Size: 2875 1156 3560 Fragments arranged by size: 3560 2875 1156 ApoI r'AATT_y Cuts at: 1493 2090 4900 5126 5278 7387 1493 Size: 597 2810 226 152 2109 1697 Fragments arranged by size: 2810 2109 1697 597 226 152 AseI AT'TA_AT Cuts at: 160 1032 6145 160 Size: 872 5113 1606 Fragments arranged by size: 5113 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7591 AvaI C'yCGr_G Cuts at: 1870 1943 2987 3035 3245 3383 3407 3431 3469 Size: 73 1044 48 210 138 24 24 38 Cuts at: 3469 3863 3922 4027 4193 4262 4354 4595 4916 Size: 394 59 105 166 69 92 241 321 Cuts at: 4916 1870 Size: 4545 Fragments arranged by size: 4545 1044 394 321 241 210 166 138 105 92 73 69 59 48 38 24 24 BamHI G'GATC_C Cuts at: 4921 5288 4921 Size: 367 7224 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2531 2614 2860 2893 3127 Size: 325 217 627 744 83 246 33 234 Cuts at: 3127 3274 3373 3460 3481 3643 3656 3670 3698 Size: 147 99 87 21 162 13 14 28 Cuts at: 3698 4097 4217 4591 4912 6367 618 Size: 399 120 374 321 1455 1842 Fragments arranged by size: 1842 1455 744 627 399 374 325 321 246 234 217 162 147 120 99 87 83 33 28 21 14 13 BanII G_rGCy'C Cuts at: 1391 2275 2468 2495 2920 3027 3036 3066 3086 Size: 884 193 27 425 107 9 30 20 Cuts at: 3086 3206 3363 3384 3408 3432 3453 3476 3530 Size: 120 157 21 24 24 21 23 54 Cuts at: 3530 3579 3870 3965 4002 4228 4383 4910 1391 Size: 49 291 95 37 226 155 527 4072 Fragments arranged by size: 4072 884 527 425 291 226 193 157 155 120 107 95 54 49 37 30 27 24 24 23 21 21 20 9 BbeI G_GCGC'C Cuts at: 2864 2897 3131 3278 3377 3464 3485 3647 3660 Size: 33 234 147 99 87 21 162 13 Cuts at: 3660 3674 4101 2864 Size: 14 427 6354 Fragments arranged by size: 6354 427 234 162 147 99 87 33 21 14 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3198 4545 928 Size: 971 1299 1347 3974 Fragments arranged by size: 3974 1347 1299 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5782 5816 1903 Size: 34 3845 34 3678 Fragments arranged by size: 3845 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5483 6693 5483 Size: 1210 6381 BclI T'GATC_A Cuts at: 1548 1548 Size: 7591 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3367 3665 3944 Size: 107 122 71 631 605 1695 298 279 Cuts at: 3944 6202 136 Size: 2258 1525 Fragments arranged by size: 2258 1695 1525 631 605 298 279 122 107 71 BglII A'GATC_T Cuts at: 7586 7586 Size: 7591 BlpI GC'TnA_GC Cuts at: 2676 4271 5008 2676 Size: 1595 737 5259 Fragments arranged by size: 5259 1595 737 Bme1580I G_kGCm'C Cuts at: 1790 2258 2275 2777 2920 3086 3151 3363 3530 Size: 468 17 502 143 166 65 212 167 Cuts at: 3530 4228 4555 4596 5652 6808 1790 Size: 698 327 41 1056 1156 2573 Fragments arranged by size: 2573 1156 1056 698 502 468 327 212 167 166 143 65 41 17 BmgBI CAC'GTC Cuts at: 1318 2521 2772 2857 1318 Size: 1203 251 85 6052 Fragments arranged by size: 6052 1203 251 85 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5322 6280 442 Size: 416 53 83 399 24 3905 958 1753 Fragments arranged by size: 3905 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7591 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3016 3295 3016 Size: 279 7312 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2406 2544 2672 3836 3844 4473 6251 Size: 1022 145 138 128 1164 8 629 1778 Cuts at: 6251 1239 Size: 2579 Fragments arranged by size: 2579 1778 1164 1022 629 145 138 128 8 Bpu10I CC'TnA_GC Cuts at: 2759 2877 3378 2759 Size: 118 501 6972 Fragments arranged by size: 6972 501 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2986 4842 5716 6581 6879 2986 Size: 1856 874 865 298 3698 Fragments arranged by size: 3698 1856 874 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2551 3863 6254 882 Size: 617 1052 1312 2391 2219 Fragments arranged by size: 2391 2219 1312 1052 617 BsaAI yAC'GTr Cuts at: 493 1204 2569 5382 493 Size: 711 1365 2813 2702 Fragments arranged by size: 2813 2702 1365 711 BsaBI GATnn'nnATC Cuts at: 7585 7585 Size: 7591 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2861 2894 2982 3128 Size: 53 83 186 936 1328 33 88 146 Cuts at: 3128 3195 3275 3329 3374 3461 3482 3644 3657 Size: 67 80 54 45 87 21 162 13 Cuts at: 3657 3671 3762 4098 4347 5398 5780 6415 275 Size: 14 91 336 249 1051 382 635 1451 Fragments arranged by size: 1451 1328 1051 936 635 382 336 249 186 162 146 91 88 87 83 80 67 54 53 45 33 21 14 13 BsaWI w'CCGG_w Cuts at: 2306 4062 4143 4680 6024 6422 6696 6843 7254 Size: 1756 81 537 1344 398 274 147 411 Cuts at: 7254 2306 Size: 2643 Fragments arranged by size: 2643 1756 1344 537 411 398 274 147 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3053 3083 4169 4199 1178 Size: 30 165 30 1650 30 1086 30 4570 Fragments arranged by size: 4570 1650 1086 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2409 2641 2798 2801 2813 2938 2987 Size: 1072 151 232 157 3 12 125 49 Cuts at: 2987 3185 3365 3429 3823 3841 4193 1186 Size: 198 180 64 394 18 352 4584 Fragments arranged by size: 4584 1072 394 352 232 198 180 157 151 125 64 49 18 12 3 BseYI C'CCAG_C Cuts at: 2260 2837 3870 4006 4229 6794 7290 2260 Size: 577 1033 136 223 2565 496 2561 Fragments arranged by size: 2565 2561 1033 577 496 223 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 1163 Size: 573 762 6256 Fragments arranged by size: 6256 762 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2714 3516 3793 3948 3978 5041 Size: 275 186 713 802 277 155 30 1063 Cuts at: 5041 5802 6830 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1063 1028 802 761 713 277 275 186 155 30 BsiHKAI G_wGCw'C Cuts at: 2468 2777 3027 3187 3342 4170 5652 5737 6808 Size: 309 250 160 155 828 1482 85 1071 Cuts at: 6808 2468 Size: 3251 Fragments arranged by size: 3251 1482 1071 828 309 250 160 155 85 BsmI GAATG_Cn' Cuts at: 5102 5195 5102 Size: 93 7498 BsmBI CGTCTCn'nnnn_ Cuts at: 2590 3392 3769 2590 Size: 802 377 6412 Fragments arranged by size: 6412 802 377 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2258 2275 2468 2495 2777 2920 3027 Size: 399 468 17 193 27 282 143 107 Cuts at: 3027 3036 3066 3086 3151 3187 3206 3342 3363 Size: 9 30 20 65 36 19 136 21 Cuts at: 3363 3384 3408 3432 3453 3476 3530 3579 3870 Size: 21 24 24 21 23 54 49 291 Cuts at: 3870 3965 4002 4170 4228 4383 4555 4596 4910 Size: 95 37 168 58 155 172 41 314 Cuts at: 4910 5652 5737 6808 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 468 399 314 291 282 193 172 168 155 143 136 107 95 85 65 58 54 49 41 37 36 30 27 24 24 23 21 21 21 20 19 17 9 BspEI T'CCGG_A Cuts at: 2306 7254 2306 Size: 4948 2643 BspHI T'CATG_A Cuts at: 5480 7210 5480 Size: 1730 5861 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4946 844 Size: 4102 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3419 3679 3813 4270 4363 5478 1394 Size: 429 1596 260 134 457 93 1115 3507 Fragments arranged by size: 3507 1596 1115 457 429 260 134 93 BsrDI GCAATG_nn' Cuts at: 65 1248 2193 6086 6260 65 Size: 1183 945 3893 174 1396 Fragments arranged by size: 3893 1396 1183 945 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2456 2890 3370 3478 3504 3649 4021 Size: 110 646 434 480 108 26 145 372 Cuts at: 4021 4349 6235 6422 1700 Size: 328 1886 187 2869 Fragments arranged by size: 2869 1886 646 480 434 372 328 187 145 110 108 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7591 BssHII G'CGCG_C Cuts at: 1925 2740 1925 Size: 815 6776 BssSI C'ACGA_G Cuts at: 3736 3991 5651 6663 3736 Size: 255 1660 1012 4664 Fragments arranged by size: 4664 1660 1012 255 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7591 BstBI TT'CG_AA Cuts at: 6395 6395 Size: 7591 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7591 BstYI r'GATC_y Cuts at: 1972 2595 4921 5288 5673 5690 7142 7228 7240 Size: 623 2326 367 385 17 1452 86 12 Cuts at: 7240 7586 1972 Size: 346 1977 Fragments arranged by size: 2326 1977 1452 623 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3225 3915 3966 3225 Size: 690 51 6850 Fragments arranged by size: 6850 690 51 BtgI C'CryG_G Cuts at: 513 1065 3156 3456 3510 513 Size: 552 2091 300 54 4594 Fragments arranged by size: 4594 2091 552 300 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2721 487 Size: 683 56 1495 5357 Fragments arranged by size: 5357 1495 683 56 BtsI GCAGTG_nn' Cuts at: 3310 4770 5107 5901 5921 3310 Size: 1460 337 794 20 4980 Fragments arranged by size: 4980 1460 794 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7591 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7556 DraI TTT'AAA Cuts at: 4895 4895 Size: 7591 DraIII CAC_nnn'GTG Cuts at: 2521 2521 Size: 7591 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2582 4564 6598 809 Size: 507 1266 1982 2034 1802 Fragments arranged by size: 2034 1982 1802 1266 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2961 3012 3153 Size: 54 1425 50 275 18 1131 51 141 Cuts at: 3153 3513 3790 3936 3945 3975 5038 5927 7556 Size: 360 277 146 9 30 1063 889 1629 Cuts at: 7556 8 Size: 43 Fragments arranged by size: 1629 1425 1131 1063 889 360 277 275 146 141 54 51 50 43 30 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3513 3790 3945 3975 5038 1537 Size: 275 1701 277 155 30 1063 4090 Fragments arranged by size: 4090 1701 1063 277 275 155 30 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2823 4118 4790 5521 1353 Size: 767 703 1295 672 731 3423 Fragments arranged by size: 3423 1295 767 731 703 672 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3123 3182 3701 3917 4406 6178 Size: 1064 138 1534 59 519 216 489 1772 Cuts at: 6178 6552 6698 387 Size: 374 146 1280 Fragments arranged by size: 1772 1534 1280 1064 519 489 374 216 146 138 59 EcoICRI GAG'CTC Cuts at: 2466 3025 2466 Size: 559 7032 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2406 2544 2614 2672 3836 3844 Size: 393 629 145 138 70 58 1164 8 Cuts at: 3844 4473 4583 4814 5654 6251 7038 7306 1239 Size: 629 110 231 840 597 787 268 1524 Fragments arranged by size: 1524 1164 840 787 629 629 597 393 268 231 145 138 110 70 58 8 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2421 2060 Size: 361 7230 EcoO109I rG'GnC_Cy Cuts at: 2272 2525 2873 2916 2995 3082 3083 3359 3360 Size: 253 348 43 79 87 1 276 1 Cuts at: 3360 3526 3527 4224 4225 4448 4512 2272 Size: 166 1 697 1 223 64 5351 Fragments arranged by size: 5351 697 348 276 253 223 166 87 79 64 43 1 1 1 1 EcoRI G'AATT_C Cuts at: 4900 5278 4900 Size: 378 7213 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4720 4720 Size: 7591 FspI TGC'GCA Cuts at: 4409 6097 4409 Size: 1688 5903 HaeII r_GCGC'y Cuts at: 1678 2836 2864 2897 3131 3278 3377 3464 3485 Size: 1158 28 33 234 147 99 87 21 Cuts at: 3485 3622 3647 3660 3674 4101 6738 1678 Size: 137 25 13 14 427 2637 2531 Fragments arranged by size: 2637 2531 1158 427 234 147 137 99 87 33 28 25 21 14 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2388 3023 3053 3055 Size: 32 135 32 784 32 635 30 2 Cuts at: 3055 3085 4085 4117 4811 4843 6232 6264 6306 Size: 30 1000 32 694 32 1389 32 42 Cuts at: 6306 6338 1373 Size: 32 2626 Fragments arranged by size: 2626 1389 1000 784 694 635 135 42 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 4935 5181 669 Size: 988 281 2997 246 3079 Fragments arranged by size: 3079 2997 988 281 246 HindIII A'AGCT_T Cuts at: 748 2033 4860 748 Size: 1285 2827 3479 Fragments arranged by size: 3479 2827 1285 HpaI GTT'AAC Cuts at: 5181 5181 Size: 7591 KasI G'GCGC_C Cuts at: 2860 2893 3127 3274 3373 3460 3481 3643 3656 Size: 33 234 147 99 87 21 162 13 Cuts at: 3656 3670 4097 2860 Size: 14 427 6354 Fragments arranged by size: 6354 427 234 162 147 99 87 33 21 14 13 KpnI G_GTAC'C Cuts at: 2535 4916 2535 Size: 2381 5210 MfeI C'AATT_G Cuts at: 5190 5190 Size: 7591 MluI A'CGCG_T Cuts at: 7467 7467 Size: 7591 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6705 6889 1305 Size: 5400 184 2007 Fragments arranged by size: 5400 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5769 Fragments arranged by size: 5769 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 5549 5908 6067 518 Size: 648 852 3531 359 159 2042 Fragments arranged by size: 3531 2042 852 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2710 3512 3554 3775 3789 4405 4673 4792 Size: 1104 802 42 221 14 616 268 119 Cuts at: 4792 5007 5684 6832 7077 1606 Size: 215 677 1148 245 2120 Fragments arranged by size: 2120 1148 1104 802 677 616 268 245 221 215 119 42 14 NaeI GCC'GGC Cuts at: 2458 3372 3480 4023 4351 2458 Size: 914 108 543 328 5698 Fragments arranged by size: 5698 914 543 328 108 NarI GG'CG_CC Cuts at: 2861 2894 3128 3275 3374 3461 3482 3644 3657 Size: 33 234 147 99 87 21 162 13 Cuts at: 3657 3671 4098 2861 Size: 14 427 6354 Fragments arranged by size: 6354 427 234 162 147 99 87 33 21 14 13 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7039 NdeI CA'TA_TG Cuts at: 387 387 Size: 7591 NgoMIV G'CCGG_C Cuts at: 2456 3370 3478 4021 4349 2456 Size: 914 108 543 328 5698 Fragments arranged by size: 5698 914 543 328 108 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7591 NotI GC'GGCC_GC Cuts at: 3513 3790 3975 5038 3513 Size: 277 185 1063 6066 Fragments arranged by size: 6066 1063 277 185 NruI TCG'CGA Cuts at: 3200 3288 3806 3200 Size: 88 518 6985 Fragments arranged by size: 6985 518 88 NspI r_CATG'y Cuts at: 1132 1229 4876 4949 6494 1132 Size: 97 3647 73 1545 2229 Fragments arranged by size: 3647 2229 1545 97 73 PasI CC'CwG_GG Cuts at: 1348 3365 1348 Size: 2017 5574 PciI A'CATG_T Cuts at: 6490 6490 Size: 7591 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7591 PfoI T'CCnGG_A Cuts at: 3342 4829 3342 Size: 1487 6104 PmeI GTTT'AAAC Cuts at: 4895 4895 Size: 7591 PmlI CAC'GTG Cuts at: 2569 2569 Size: 7591 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5660 5692 7173 7205 5660 Size: 32 1481 32 6046 Fragments arranged by size: 6046 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2525 2995 4512 2525 Size: 470 1517 5604 Fragments arranged by size: 5604 1517 470 PshAI GACnn'nnGTC Cuts at: 1613 2523 1613 Size: 910 6681 PsiI TTA'TAA Cuts at: 4886 5161 4886 Size: 275 7316 PspOMI G'GGCC_C Cuts at: 2271 2916 3082 3359 3526 4224 2271 Size: 645 166 277 167 698 5638 Fragments arranged by size: 5638 698 645 277 167 166 PstI C_TGCA'G Cuts at: 830 2050 2379 2644 2674 2943 4311 4441 4765 Size: 1220 329 265 30 269 1368 130 324 Cuts at: 4765 4943 830 Size: 178 3478 Fragments arranged by size: 3478 1368 1220 329 324 269 265 178 130 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7591 PvuII CAG'CTG Cuts at: 4792 4792 Size: 7591 SacI G_AGCT'C Cuts at: 2468 3027 2468 Size: 559 7032 SacII CC_GC'GG Cuts at: 3513 3513 Size: 7591 SalI G'TCGA_C Cuts at: 1936 4933 1936 Size: 2997 4594 SapI GCTCTTCn'nnn_ Cuts at: 2823 2823 Size: 7591 SbfI CC_TGCA'GG Cuts at: 4943 4943 Size: 7591 ScaI AGT'ACT Cuts at: 5839 7551 5839 Size: 1712 5879 SfcI C'TryA_G Cuts at: 826 1045 2046 2375 2640 2670 2939 4307 4437 Size: 219 1001 329 265 30 269 1368 130 Cuts at: 4437 4761 4939 6074 6755 6946 826 Size: 324 178 1135 681 191 1471 Fragments arranged by size: 1471 1368 1135 1001 681 329 324 269 265 219 191 178 130 30 SfiI GGCCn_nnn'nGGCC Cuts at: 3367 3944 3367 Size: 577 7014 SfoI GGC'GCC Cuts at: 2862 2895 3129 3276 3375 3462 3483 3645 3658 Size: 33 234 147 99 87 21 162 13 Cuts at: 3658 3672 4099 2862 Size: 14 427 6354 Fragments arranged by size: 6354 427 234 162 147 99 87 33 21 14 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7591 SgrAI Cr'CCGG_yG Cuts at: 1700 2890 3478 3649 1700 Size: 1190 588 171 5642 Fragments arranged by size: 5642 1190 588 171 SmaI CCC'GGG Cuts at: 1872 2989 4356 4918 1872 Size: 1117 1367 562 4545 Fragments arranged by size: 4545 1367 1117 562 SmlI C'TyrA_G Cuts at: 820 1017 1943 3001 4857 5695 6596 6858 820 Size: 197 926 1058 1856 838 901 262 1553 Fragments arranged by size: 1856 1553 1058 926 901 838 262 197 SnaBI TAC'GTA Cuts at: 493 5382 493 Size: 4889 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7591 SphI G_CATG'C Cuts at: 1132 4949 1132 Size: 3817 3774 SspI AAT'ATT Cuts at: 5 52 5515 5 Size: 47 5463 2081 Fragments arranged by size: 5463 2081 47 StuI AGG'CCT Cuts at: 1517 2055 1517 Size: 538 7053 StyI C'CwwG_G Cuts at: 513 1065 2056 3624 4237 4515 4587 5030 513 Size: 552 991 1568 613 278 72 443 3074 Fragments arranged by size: 3074 1568 991 613 552 443 278 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3440 3461 4543 5618 5635 5788 5973 Size: 1255 1860 21 1082 1075 17 153 185 Cuts at: 5973 6418 325 Size: 445 1498 Fragments arranged by size: 1860 1498 1255 1082 1075 445 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4814 5837 7549 Size: 274 80 33 51 1443 2837 1023 1712 Cuts at: 7549 96 Size: 138 Fragments arranged by size: 2837 1712 1443 1023 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4169 4201 7551 7583 4169 Size: 32 3350 32 4177 Fragments arranged by size: 4177 3350 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7591 XbaI T'CTAG_A Cuts at: 4927 4927 Size: 7591 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 3055 2020 Size: 1035 6556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7591 XmaI C'CCGG_G Cuts at: 1870 2987 4354 4916 1870 Size: 1117 1367 562 4545 Fragments arranged by size: 4545 1367 1117 562 XmnI GAAnn'nnTTC Cuts at: 3722 5720 3722 Size: 1998 5593 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3196 5399 276 Size: 53 83 186 936 1662 2203 2468 Fragments arranged by size: 2468 2203 1662 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I EcoRV FseI FspAI NsiI PacI PspXI PsrI RsrII SanDI SexAI SrfI SwaI