(Linear) (Six-base) MAPSORT of: KIEE2355.seq Check: 8724 from: 1 to: 2886 KIEE2355.orf With 182 enzymes: SgfI * June 21, 2011 13:28 .. AatII G_ACGT'C Cuts at: 0 1193 2886 Size: 1193 1693 Acc65I G'GTAC_C Cuts at: 0 526 2886 Size: 526 2360 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 609 2578 2809 2886 Size: 609 1969 231 77 Fragments arranged by size: 1969 609 231 77 AleI CACnn'nnGTG Cuts at: 0 13 2886 Size: 13 2873 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1286 1318 2886 Size: 1286 32 1568 Fragments arranged by size: 1568 1286 32 AlwNI CAG_nnn'CTG Cuts at: 0 619 2446 2886 Size: 619 1827 440 Fragments arranged by size: 1827 619 440 ApaI G_GGCC'C Cuts at: 0 270 915 1081 1358 1525 2223 2886 Size: 270 645 166 277 167 698 663 Fragments arranged by size: 698 663 645 277 270 167 166 ApaLI G'TGCA_C Cuts at: 0 768 2886 Size: 768 2118 ApoI r'AATT_y Cuts at: 0 85 2886 Size: 85 2801 AvaI C'yCGr_G Cuts at: 0 982 1030 1240 1378 1402 1426 1464 1858 Size: 982 48 210 138 24 24 38 394 Cuts at: 1858 1917 2022 2188 2257 2349 2590 2886 Size: 59 105 166 69 92 241 296 Fragments arranged by size: 982 394 296 241 210 166 138 105 92 69 59 48 38 24 24 BanI G'GyrC_C Cuts at: 0 526 609 855 888 1122 1269 1368 1455 Size: 526 83 246 33 234 147 99 87 Cuts at: 1455 1476 1638 1651 1665 1693 2092 2212 2586 Size: 21 162 13 14 28 399 120 374 Cuts at: 2586 2886 Size: 300 Fragments arranged by size: 526 399 374 300 246 234 162 147 120 99 87 83 33 28 21 14 13 BanII G_rGCy'C Cuts at: 0 270 463 490 915 1022 1031 1061 1081 Size: 270 193 27 425 107 9 30 20 Cuts at: 1081 1201 1358 1379 1403 1427 1448 1471 1525 Size: 120 157 21 24 24 21 23 54 Cuts at: 1525 1574 1865 1960 1997 2223 2378 2886 Size: 49 291 95 37 226 155 508 Fragments arranged by size: 508 425 291 270 226 193 157 155 120 107 95 54 49 37 30 27 24 24 23 21 21 20 9 BbeI G_GCGC'C Cuts at: 0 859 892 1126 1273 1372 1459 1480 1642 Size: 859 33 234 147 99 87 21 162 Cuts at: 1642 1655 1669 2096 2886 Size: 13 14 427 790 Fragments arranged by size: 859 790 427 234 162 147 99 87 33 21 14 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 1193 2540 2886 Size: 1193 1347 346 Fragments arranged by size: 1347 1193 346 BglI GCCn_nnn'nGGC Cuts at: 0 1362 1660 1939 2886 Size: 1362 298 279 947 Fragments arranged by size: 1362 947 298 279 BlpI GC'TnA_GC Cuts at: 0 671 2266 2886 Size: 671 1595 620 Fragments arranged by size: 1595 671 620 Bme1580I G_kGCm'C Cuts at: 0 253 270 772 915 1081 1146 1358 1525 Size: 253 17 502 143 166 65 212 167 Cuts at: 1525 2223 2550 2591 2886 Size: 698 327 41 295 Fragments arranged by size: 698 502 327 295 253 212 167 166 143 65 41 17 BmgBI CAC'GTC Cuts at: 0 516 767 852 2886 Size: 516 251 85 2034 Fragments arranged by size: 2034 516 251 85 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1011 1290 2886 Size: 1011 279 1596 Fragments arranged by size: 1596 1011 279 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 401 539 667 1831 1839 2468 2886 Size: 256 145 138 128 1164 8 629 418 Fragments arranged by size: 1164 629 418 256 145 138 128 8 Bpu10I CC'TnA_GC Cuts at: 0 754 872 1373 2886 Size: 754 118 501 1513 Fragments arranged by size: 1513 754 501 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 981 2837 2886 Size: 981 1856 49 Fragments arranged by size: 1856 981 49 BsaI GGTCTCn'nnnn_ Cuts at: 0 546 1858 2886 Size: 546 1312 1028 Fragments arranged by size: 1312 1028 546 BsaAI yAC'GTr Cuts at: 0 564 2886 Size: 564 2322 BsaHI Gr'CG_yC Cuts at: 0 856 889 977 1123 1190 1270 1324 1369 Size: 856 33 88 146 67 80 54 45 Cuts at: 1369 1456 1477 1639 1652 1666 1757 2093 2342 Size: 87 21 162 13 14 91 336 249 Cuts at: 2342 2886 Size: 544 Fragments arranged by size: 856 544 336 249 162 146 91 88 87 80 67 54 45 33 21 14 13 BsaWI w'CCGG_w Cuts at: 0 301 2057 2138 2675 2886 Size: 301 1756 81 537 211 Fragments arranged by size: 1756 537 301 211 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1048 1078 2164 2194 2886 Size: 1048 30 1086 30 692 Fragments arranged by size: 1086 1048 692 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 253 404 636 793 796 808 933 982 Size: 253 151 232 157 3 12 125 49 Cuts at: 982 1180 1360 1424 1818 1836 2188 2886 Size: 198 180 64 394 18 352 698 Fragments arranged by size: 698 394 352 253 232 198 180 157 151 125 64 49 18 12 3 BseYI C'CCAG_C Cuts at: 0 255 832 1865 2001 2224 2886 Size: 255 577 1033 136 223 662 Fragments arranged by size: 1033 662 577 255 223 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 2886 Size: 493 2393 BsiEI CG_ry'CG Cuts at: 0 709 1511 1788 1943 1973 2886 Size: 709 802 277 155 30 913 Fragments arranged by size: 913 802 709 277 155 30 BsiHKAI G_wGCw'C Cuts at: 0 463 772 1022 1182 1337 2165 2886 Size: 463 309 250 160 155 828 721 Fragments arranged by size: 828 721 463 309 250 160 155 BsmBI CGTCTCn'nnnn_ Cuts at: 0 585 1387 1764 2886 Size: 585 802 377 1122 Fragments arranged by size: 1122 802 585 377 Bsp1286I G_dGCh'C Cuts at: 0 253 270 463 490 772 915 1022 1031 Size: 253 17 193 27 282 143 107 9 Cuts at: 1031 1061 1081 1146 1182 1201 1337 1358 1379 Size: 30 20 65 36 19 136 21 21 Cuts at: 1379 1403 1427 1448 1471 1525 1574 1865 1960 Size: 24 24 21 23 54 49 291 95 Cuts at: 1960 1997 2165 2223 2378 2550 2591 2886 Size: 37 168 58 155 172 41 295 Fragments arranged by size: 295 291 282 253 193 172 168 155 143 136 107 95 65 58 54 49 41 37 36 30 27 24 24 23 21 21 21 20 19 17 9 BspEI T'CCGG_A Cuts at: 0 301 2886 Size: 301 2585 BsrBI CCG'CTC Cuts at: 0 1414 1674 1808 2265 2358 2886 Size: 1414 260 134 457 93 528 Fragments arranged by size: 1414 528 457 260 134 93 BsrDI GCAATG_nn' Cuts at: 0 188 2886 Size: 188 2698 BsrFI r'CCGG_y Cuts at: 0 451 885 1365 1473 1499 1644 2016 2344 Size: 451 434 480 108 26 145 372 328 Cuts at: 2344 2886 Size: 542 Fragments arranged by size: 542 480 451 434 372 328 145 108 26 BssHII G'CGCG_C Cuts at: 0 735 2886 Size: 735 2151 BssSI C'ACGA_G Cuts at: 0 1731 1986 2886 Size: 1731 255 900 Fragments arranged by size: 1731 900 255 BstYI r'GATC_y Cuts at: 0 590 2886 Size: 590 2296 Bsu36I CC'TnA_GG Cuts at: 0 1220 1910 1961 2886 Size: 1220 690 51 925 Fragments arranged by size: 1220 925 690 51 BtgI C'CryG_G Cuts at: 0 1151 1451 1505 2886 Size: 1151 300 54 1381 Fragments arranged by size: 1381 1151 300 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 716 2886 Size: 716 2170 BtsI GCAGTG_nn' Cuts at: 0 1305 2765 2886 Size: 1305 1460 121 Fragments arranged by size: 1460 1305 121 DraIII CAC_nnn'GTG Cuts at: 0 516 2886 Size: 516 2370 DrdI GACnn_nn'nnGTC Cuts at: 0 577 2559 2886 Size: 577 1982 327 Fragments arranged by size: 1982 577 327 EaeI y'GGCC_r Cuts at: 0 956 1007 1148 1508 1785 1931 1940 1970 Size: 956 51 141 360 277 146 9 30 Cuts at: 1970 2886 Size: 916 Fragments arranged by size: 956 916 360 277 146 141 51 30 9 EagI C'GGCC_G Cuts at: 0 1508 1785 1940 1970 2886 Size: 1508 277 155 30 916 Fragments arranged by size: 1508 916 277 155 30 EarI CTCTTCn'nnn_ Cuts at: 0 115 818 2113 2785 2886 Size: 115 703 1295 672 101 Fragments arranged by size: 1295 703 672 115 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1118 1177 1696 1912 2401 2886 Size: 1118 59 519 216 489 485 Fragments arranged by size: 1118 519 489 485 216 59 EcoICRI GAG'CTC Cuts at: 0 461 1020 2886 Size: 461 559 1866 Fragments arranged by size: 1866 559 461 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 401 539 609 667 1831 1839 2468 Size: 256 145 138 70 58 1164 8 629 Cuts at: 2468 2578 2809 2886 Size: 110 231 77 Fragments arranged by size: 1164 629 256 231 145 138 110 77 70 58 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 55 416 2886 Size: 55 361 2470 Fragments arranged by size: 2470 361 55 EcoO109I rG'GnC_Cy Cuts at: 0 267 520 868 911 990 1077 1078 1354 Size: 267 253 348 43 79 87 1 276 Cuts at: 1354 1355 1521 1522 2219 2220 2443 2507 2886 Size: 1 166 1 697 1 223 64 379 Fragments arranged by size: 697 379 348 276 267 253 223 166 87 79 64 43 1 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2715 2886 Size: 2715 171 FspI TGC'GCA Cuts at: 0 2404 2886 Size: 2404 482 HaeII r_GCGC'y Cuts at: 0 831 859 892 1126 1273 1372 1459 1480 Size: 831 28 33 234 147 99 87 21 Cuts at: 1480 1617 1642 1655 1669 2096 2886 Size: 137 25 13 14 427 790 Fragments arranged by size: 831 790 427 234 147 137 99 87 33 28 25 21 14 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 351 383 1018 1048 1050 1080 2080 2112 Size: 351 32 635 30 2 30 1000 32 Cuts at: 2112 2806 2838 2886 Size: 694 32 48 Fragments arranged by size: 1000 694 635 351 48 32 32 32 30 30 2 HindIII A'AGCT_T Cuts at: 0 28 2855 2886 Size: 28 2827 31 Fragments arranged by size: 2827 31 28 KasI G'GCGC_C Cuts at: 0 855 888 1122 1269 1368 1455 1476 1638 Size: 855 33 234 147 99 87 21 162 Cuts at: 1638 1651 1665 2092 2886 Size: 13 14 427 794 Fragments arranged by size: 855 794 427 234 162 147 99 87 33 21 14 13 KpnI G_GTAC'C Cuts at: 0 530 2886 Size: 530 2356 MslI CAynn'nnrTG Cuts at: 0 13 2886 Size: 13 2873 MspA1I CmG'CkG Cuts at: 0 705 1507 1549 1770 1784 2400 2668 2787 Size: 705 802 42 221 14 616 268 119 Cuts at: 2787 2886 Size: 99 Fragments arranged by size: 802 705 616 268 221 119 99 42 14 NaeI GCC'GGC Cuts at: 0 453 1367 1475 2018 2346 2886 Size: 453 914 108 543 328 540 Fragments arranged by size: 914 543 540 453 328 108 NarI GG'CG_CC Cuts at: 0 856 889 1123 1270 1369 1456 1477 1639 Size: 856 33 234 147 99 87 21 162 Cuts at: 1639 1652 1666 2093 2886 Size: 13 14 427 793 Fragments arranged by size: 856 793 427 234 162 147 99 87 33 21 14 13 NgoMIV G'CCGG_C Cuts at: 0 451 1365 1473 2016 2344 2886 Size: 451 914 108 543 328 542 Fragments arranged by size: 914 543 542 451 328 108 NotI GC'GGCC_GC Cuts at: 0 1508 1785 1970 2886 Size: 1508 277 185 916 Fragments arranged by size: 1508 916 277 185 NruI TCG'CGA Cuts at: 0 1195 1283 1801 2886 Size: 1195 88 518 1085 Fragments arranged by size: 1195 1085 518 88 NspI r_CATG'y Cuts at: 0 2871 2886 Size: 2871 15 PasI CC'CwG_GG Cuts at: 0 1360 2886 Size: 1360 1526 PfoI T'CCnGG_A Cuts at: 0 1337 2824 2886 Size: 1337 1487 62 Fragments arranged by size: 1487 1337 62 PmlI CAC'GTG Cuts at: 0 564 2886 Size: 564 2322 PpuMI rG'GwC_Cy Cuts at: 0 520 990 2507 2886 Size: 520 470 1517 379 Fragments arranged by size: 1517 520 470 379 PshAI GACnn'nnGTC Cuts at: 0 518 2886 Size: 518 2368 PsiI TTA'TAA Cuts at: 0 2881 2886 Size: 2881 5 PspOMI G'GGCC_C Cuts at: 0 266 911 1077 1354 1521 2219 2886 Size: 266 645 166 277 167 698 667 Fragments arranged by size: 698 667 645 277 266 167 166 PstI C_TGCA'G Cuts at: 0 45 374 639 669 938 2306 2436 2760 Size: 45 329 265 30 269 1368 130 324 Cuts at: 2760 2886 Size: 126 Fragments arranged by size: 1368 329 324 269 265 130 126 45 30 PvuII CAG'CTG Cuts at: 0 2787 2886 Size: 2787 99 SacI G_AGCT'C Cuts at: 0 463 1022 2886 Size: 463 559 1864 Fragments arranged by size: 1864 559 463 SacII CC_GC'GG Cuts at: 0 1508 2886 Size: 1508 1378 SapI GCTCTTCn'nnn_ Cuts at: 0 818 2886 Size: 818 2068 SfcI C'TryA_G Cuts at: 0 41 370 635 665 934 2302 2432 2756 Size: 41 329 265 30 269 1368 130 324 Cuts at: 2756 2886 Size: 130 Fragments arranged by size: 1368 329 324 269 265 130 130 41 30 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1362 1939 2886 Size: 1362 577 947 Fragments arranged by size: 1362 947 577 SfoI GGC'GCC Cuts at: 0 857 890 1124 1271 1370 1457 1478 1640 Size: 857 33 234 147 99 87 21 162 Cuts at: 1640 1653 1667 2094 2886 Size: 13 14 427 792 Fragments arranged by size: 857 792 427 234 162 147 99 87 33 21 14 13 SgrAI Cr'CCGG_yG Cuts at: 0 885 1473 1644 2886 Size: 885 588 171 1242 Fragments arranged by size: 1242 885 588 171 SmaI CCC'GGG Cuts at: 0 984 2351 2886 Size: 984 1367 535 Fragments arranged by size: 1367 984 535 SmlI C'TyrA_G Cuts at: 0 996 2852 2886 Size: 996 1856 34 Fragments arranged by size: 1856 996 34 StuI AGG'CCT Cuts at: 0 50 2886 Size: 50 2836 StyI C'CwwG_G Cuts at: 0 51 1619 2232 2510 2582 2886 Size: 51 1568 613 278 72 304 Fragments arranged by size: 1568 613 304 278 72 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1435 1456 2538 2886 Size: 1435 21 1082 348 Fragments arranged by size: 1435 1082 348 21 TatI w'GTAC_w Cuts at: 0 2809 2886 Size: 2809 77 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2164 2196 2886 Size: 2164 32 690 Fragments arranged by size: 2164 690 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 15 1050 2886 Size: 15 1035 1836 Fragments arranged by size: 1836 1035 15 XmaI C'CCGG_G Cuts at: 0 982 2349 2886 Size: 982 1367 537 Fragments arranged by size: 1367 982 537 XmnI GAAnn'nnTTC Cuts at: 0 1717 2886 Size: 1717 1169 ZraI GAC'GTC Cuts at: 0 1191 2886 Size: 1191 1695 Enzymes that do cut: AatII Acc65I AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BssSI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII KasI KpnI MslI MspA1I NaeI NarI NgoMIV NotI NruI NspI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SacII SapI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI BsaBI BsiWI BsmI BspHI BspMI BsrGI BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI EcoRI EcoRV FseI FspAI HincII HpaI MfeI MluI MmeI MscI NcoI NdeI NheI NsiI PacI PciI PflMI PmeI PpiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI