Restriction map for
pFN21AE3193
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI BamHI BbrPI
BbvCI BcgI BciVI BclI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I CelII ClaI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MaeII
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 826 CTTAAG C^TTAAG
AlwI 738 GATCC GGATC(4/5)
AocI 1135 CCTGAGG CC^T[ACGT]AGG
Aor51HI 432 AGCGCT AGC^GCT
AviII 565 TGCGCA TGC^GCA
AvrII 138 CCTAGG C^CTAGG
BfaI 139 CTAG C^TAG
BfrI 826 CTTAAG C^TTAAG
BglII 878 AGATCT A^GATCT
BlnI 138 CCTAGG C^CTAGG
BsaI 1042 GGTCTC GGTCTC(1/5)
BsmAI 1043 GTCTC GTCTC(1/5)
BsmFI 583 GTCCC GGGAC(10/14)
BstEII 801 GGTGACC G^GT[ACGT]ACC
BstPI 801 GGTGACC G^GT[ACGT]ACC
BstXI 623 CCAGTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1135 CCTGAGG CC^T[ACGT]AGG
CpoI 641 CGGACCG CG^G[AT]CCG
EarI 908 GAAGAG CTCTTC(1/4)
Eco47III 432 AGCGCT AGC^GCT
Eco81I 1135 CCTGAGG CC^T[ACGT]AGG
EcoNI 525 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 801 GGTGACC G^GT[ACGT]ACC
FspI 565 TGCGCA TGC^GCA
HincII 718 GTCGAC GT[CT]^[GA]AC
HindII 718 GTCGAC GT[CT]^[GA]AC
Ksp632I 908 GAAGAG CTCTTC(1/4)
KspI 309 CCGCGG CCGC^GG
MaeI 139 CTAG C^TAG
MslI 1026 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 77 CCATGG C^CATGG
PaeR7I 439 CTCGAG C^TCGAG
PleI 1131 GAGTC GAGTC(4/5)
RsrII 641 CGGACCG CG^G[AT]CCG
SacII 309 CCGCGG CCGC^GG
SalI 718 GTCGAC G^TCGAC
SapI 908 GAAGAGC GCTCTTC(1/4)
SexAI 713 ACCTGGT A^CC[AT]GGT
Tsp509I 931 AATT ^AATT
XcmI 767 CCATCGAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 439 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 718 GTCGAC GT^[AC][GT]AC
985 GTAGAC
ApaI 199 GGGCCC GGGCC^C
257 GGGCCC
AspI 712 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
804 GACCCAGTC
BalI 864 TGGCCA TGG^CCA
1084 TGGCCA
BbeI 142 GGCGCC GGCGC^C
451 GGCGCC
BbsI 1176 GAAGAC GAAGAC(2/6)
634 GTCTTC
BpuAI 1176 GAAGAC GAAGAC(2/6)
634 GTCTTC
BseRI 670 GAGGAG GAGGAG(10/8)
1138 GAGGAG
BstYI 737 AGATCC [GA]^GATC[CT]
878 AGATCT
DpnII 738 GATC ^GATC
879 GATC
DraIII 601 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1069 CACCCTGTG
EcoT14I 77 CCATGG C^C[AT][AT]GG
138 CCTAGG
KasI 142 GGCGCC G^GCGCC
451 GGCGCC
MboI 738 GATC ^GATC
879 GATC
MflI 737 AGATCC [GA]^GATC[CT]
878 AGATCT
MluNI 864 TGGCCA TGG^CCA
1084 TGGCCA
MscI 864 TGGCCA TGG^CCA
1084 TGGCCA
NarI 142 GGCGCC GG^CGCC
451 GGCGCC
NdeII 738 GATC ^GATC
879 GATC
PflFI 712 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
804 GACCCAGTC
PflMI 807 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGCGGATGG
PpuMI 1091 AGGACCT [GA]G^G[AT]CC[CT]
1298 AGGTCCT
Sau3AI 738 GATC ^GATC
879 GATC
SfaNI 781 GATGC GCATC(5/9)
1276 GATGC
SfoI 142 GGCGCC GGC^GCC
451 GGCGCC
StyI 77 CCATGG C^C[AT][AT]GG
138 CCTAGG
Tth111I 712 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
804 GACCCAGTC
Van91I 807 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGCGGATGG
XhoII 737 AGATCC [GA]^GATC[CT]
878 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV PstI PvuII
RsaI Sau96I ScrFI SfcI SmaI
SmlI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XmaCI XmaI