################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DpnII DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 361 GACGTC GACGT^C AccII 560 CGCG CG^CG AcyI 361 GACGTC G[GA]^CG[CT]C AfaI 477 GTAC GT^AC AocI 255 CCTGAGG CC^T[ACGT]AGG ApaI 442 GGGCCC GGGCC^C BanI 221 GGTGCC G^G[CT][GA]CC BbvCI 427 CCTCAGC CCTCAGC(-5/-2) BciVI 419 GTATCC GTATCC(6/5) BfaI 7 CTAG C^TAG BlpI 298 GCTGAGC GC^T[ACGT]AGC Bpu1102I 298 GCTGAGC GC^T[ACGT]AGC BsaHI 361 GACGTC G[GA]^CG[CT]C BsaI 563 GAGACC GGTCTC(1/5) BseRI 553 CTCCTC GAGGAG(10/8) BsrFI 479 ACCGGC [GA]^CCGG[CT] BstF5I 108 GGATG GGATG(2/0) BstUI 560 CGCG CG^CG BstXI 284 CCATGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 255 CCTGAGG CC^T[ACGT]AGG CelII 298 GCTGAGC GC^T[ACGT]AGC CfoI 279 GCGC GCG^C Cfr10I 479 ACCGGC [GA]^CCGG[CT] DrdI 589 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 47 TGGCCG [CT]^GGCC[GA] Eco81I 255 CCTGAGG CC^T[ACGT]AGG EcoNI 167 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 284 CCATGG C^C[AT][AT]GG FauI 230 CCCGC CCCGC(4/6) FokI 108 GGATG GGATG(9/13) HaeII 278 AGCGCC [GA]GCGC^[CT] HgaI 312 GACGC GACGC(5/10) HhaI 279 GCGC GCG^C Hin1I 361 GACGTC G[GA]^CG[CT]C HinP1I 279 GCGC G^CGC MaeI 7 CTAG C^TAG MaeII 362 ACGT A^CGT MseI 170 TTAA T^TAA MslI 27 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 560 CGCG CG^CG NcoI 284 CCATGG C^CATGG PaeR7I 520 CTCGAG C^TCGAG PflMI 242 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 262 CAGCTG CAG^CTG RsaI 477 GTAC GT^AC SmaI 452 CCCGGG CCC^GGG SmlI 520 CTCGAG C^T[CT][GA]AG StyI 284 CCATGG C^C[AT][AT]GG TaqI 521 TCGA T^CGA Tru9I 170 TTAA T^TAA Tsp509I 571 AATT ^AATT TthHB8I 521 TCGA T^CGA Van91I 242 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 520 CTCGAG C^TCGAG XmaCI 452 CCCGGG C^CCGGG XmaI 452 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaI 452 CCCGGG C^[CT]CG[GA]G 520 CTCGAG BanII 98 GGGCTC G[GA]GC[CT]^C 442 GGGCCC BmyI 98 GGGCTC G[AGT]GC[ACT]^C 442 GGGCCC BsgI 24 CTGCAC GTGCAG(16/14) 388 CTGCAC BsmAI 563 GAGAC GTCTC(1/5) 147 GTCTC BsoBI 452 CCCGGG C^[CT]CG[GA]G 520 CTCGAG Bsp1286I 98 GGGCTC G[AGT]GC[ACT]^C 442 GGGCCC BspMI 218 GCAGGT ACCTGC(4/8) 267 GCAGGT HinfI 196 GAGTC G^A[ACGT]TC 505 GAGTC HphI 270 GGTGA GGTGA(8/7) 533 TCACC MaeIII 64 GTGAC ^GT[ACGT]AC 532 GTCAC MspA1I 262 CAGCTG C[AC]G^C[GT]G 335 CCGCTG NlaIII 12 CATG CATG^ 285 CATG PleI 196 GAGTC GAGTC(4/5) 505 GAGTC SfaNI 109 GATGC GCATC(5/9) 184 GATGC StuI 164 AGGCCT AGG^CCT 524 AGGCCT Tsp45I 64 GTGAC ^GT[GC]AC 532 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BcnI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII DsaI EarI EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI Ksp632I MboII MnlI MspI MvaI MwoI NciI NlaIV PpuMI PstI Sau96I ScrFI SfcI TseI TspRI