(Circular) (Six-base) MAPSORT of: pFN21AE1691.seq Check: 6811 from: 1 to: 5305 pFN21AE1691 5305 bp With 182 enzymes: SgfI * March 28, 2012 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2214 2263 2214 Size: 49 5256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2370 3115 278 Size: 53 83 186 936 834 745 2468 Fragments arranged by size: 2468 936 834 745 186 83 53 AccI GT'mk_AC Cuts at: 1937 2648 1937 Size: 711 4594 Acc65I G'GTAC_C Cuts at: 2626 2626 Size: 5305 AclI AA'CG_TT Cuts at: 1563 3432 3805 1563 Size: 1869 373 3063 Fragments arranged by size: 3063 1869 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 2342 3368 4752 5020 1632 Size: 602 108 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1026 602 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5108 AflIII A'CryG_T Cuts at: 1203 4204 5181 1203 Size: 3001 977 1327 Fragments arranged by size: 3001 1327 977 AgeI A'CCGG_T Cuts at: 4136 4136 Size: 5305 AhdI GACnn_n'nnGTC Cuts at: 4034 4034 Size: 5305 AleI CACnn'nnGTG Cuts at: 1166 2036 1166 Size: 870 4435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2054 2140 1184 Size: 870 86 4349 Fragments arranged by size: 4349 870 86 AlwNI CAG_nnn'CTG Cuts at: 2084 2164 2253 3048 4620 2084 Size: 80 89 795 1572 2769 Fragments arranged by size: 2769 1572 795 89 80 ApaI G_GGCC'C Cuts at: 2451 2451 Size: 5305 ApaLI G'TGCA_C Cuts at: 3362 4518 3362 Size: 1156 4149 ApoI r'AATT_y Cuts at: 1493 2614 2840 2992 5101 1493 Size: 1121 226 152 2109 1697 Fragments arranged by size: 2109 1697 1121 226 152 AseI AT'TA_AT Cuts at: 160 1032 3859 160 Size: 872 2827 1606 Fragments arranged by size: 2827 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5305 AvaI C'yCGr_G Cuts at: 1870 1943 2457 2525 2630 1870 Size: 73 514 68 105 4545 Fragments arranged by size: 4545 514 105 73 68 BamHI G'GATC_C Cuts at: 2635 3002 2635 Size: 367 4938 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2226 2626 4081 618 Size: 325 217 627 439 400 1455 1842 Fragments arranged by size: 1842 1455 627 439 400 325 217 BanII G_rGCy'C Cuts at: 1391 2107 2451 2624 1391 Size: 716 344 173 4072 Fragments arranged by size: 4072 716 344 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4334 BbvCI CC'TCA_GC Cuts at: 2433 2433 Size: 5305 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3496 3530 1903 Size: 34 1559 34 3678 Fragments arranged by size: 3678 1559 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2435 3197 4407 2435 Size: 762 1210 3333 Fragments arranged by size: 3333 1210 762 BclI T'GATC_A Cuts at: 1548 1548 Size: 5305 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3916 136 Size: 107 122 71 631 605 2244 1525 Fragments arranged by size: 2244 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5300 5300 Size: 5305 BlpI GC'TnA_GC Cuts at: 2304 2722 2304 Size: 418 4887 Bme1580I G_kGCm'C Cuts at: 1790 2451 3366 4522 1790 Size: 661 915 1156 2573 Fragments arranged by size: 2573 1156 915 661 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5305 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2031 2131 2404 Size: 416 53 83 399 24 614 100 273 Cuts at: 2404 3036 3994 442 Size: 632 958 1753 Fragments arranged by size: 1753 958 632 614 416 399 273 100 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5305 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2351 2474 3965 1239 Size: 855 257 123 1491 2579 Fragments arranged by size: 2579 1491 855 257 123 Bpu10I CC'TnA_GC Cuts at: 2433 2433 Size: 5305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3430 4295 4593 3430 Size: 865 298 4142 Fragments arranged by size: 4142 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2562 3968 882 Size: 617 1063 1406 2219 Fragments arranged by size: 2219 1406 1063 617 BsaAI yAC'GTr Cuts at: 493 1204 3096 493 Size: 711 1892 2702 Fragments arranged by size: 2702 1892 711 BsaBI GATnn'nnATC Cuts at: 5299 5299 Size: 5305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3112 3494 4129 Size: 53 83 186 936 834 745 382 635 Cuts at: 4129 275 Size: 1451 Fragments arranged by size: 1451 936 834 745 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3738 4136 4410 4557 4968 3738 Size: 398 274 147 411 4075 Fragments arranged by size: 4075 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5080 Fragments arranged by size: 5080 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 1186 Size: 1363 3942 BseYI C'CCAG_C Cuts at: 2353 4508 5004 2353 Size: 2155 496 2654 Fragments arranged by size: 2654 2155 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2014 2378 1163 Size: 573 278 364 4090 Fragments arranged by size: 4090 573 364 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2755 3516 4544 1540 Size: 275 186 754 761 1028 2301 Fragments arranged by size: 2301 1028 761 754 275 186 BsiHKAI G_wGCw'C Cuts at: 3366 3451 4522 3366 Size: 85 1071 4149 Fragments arranged by size: 4149 1071 85 BsmI GAATG_Cn' Cuts at: 2816 2909 2816 Size: 93 5212 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2451 2624 3366 3451 4522 1391 Size: 399 317 344 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 317 173 85 BspEI T'CCGG_A Cuts at: 4968 4968 Size: 5305 BspHI T'CATG_A Cuts at: 3194 4924 3194 Size: 1730 3575 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 2263 2660 844 Size: 1370 49 397 3489 Fragments arranged by size: 3489 1370 397 49 BsrBI CCG'CTC Cuts at: 1394 1823 3192 1394 Size: 429 1369 3507 Fragments arranged by size: 3507 1369 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3800 3974 65 Size: 1183 2552 174 1396 Fragments arranged by size: 2552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2484 3949 4136 1700 Size: 110 674 1465 187 2869 Fragments arranged by size: 2869 1465 674 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5305 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5305 BssSI C'ACGA_G Cuts at: 3365 4377 3365 Size: 1012 4293 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5305 BstBI TT'CG_AA Cuts at: 4109 4109 Size: 5305 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5305 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 5305 BstYI r'GATC_y Cuts at: 1972 2635 3002 3387 3404 4856 4942 4954 5300 Size: 663 367 385 17 1452 86 12 346 Cuts at: 5300 1972 Size: 1977 Fragments arranged by size: 1977 1452 663 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2261 2261 Size: 5305 BtgI C'CryG_G Cuts at: 513 1065 2098 2289 2439 513 Size: 552 1033 191 150 3379 Fragments arranged by size: 3379 1033 552 191 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4566 Fragments arranged by size: 4566 683 56 BtsI GCAGTG_nn' Cuts at: 2064 2821 3615 3635 2064 Size: 757 794 20 3734 Fragments arranged by size: 3734 794 757 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5305 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5270 DraI TTT'AAA Cuts at: 2609 2609 Size: 5305 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 4312 809 Size: 507 1284 1712 1802 Fragments arranged by size: 1802 1712 1284 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2752 3641 Size: 54 1425 50 275 18 222 700 889 Cuts at: 3641 5270 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 700 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2752 1537 Size: 275 940 4090 Fragments arranged by size: 4090 940 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2193 2246 2555 3235 1353 Size: 840 53 309 680 3423 Fragments arranged by size: 3423 840 680 309 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2543 3892 4266 4412 387 Size: 1064 138 954 1349 374 146 1280 Fragments arranged by size: 1349 1280 1064 954 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2234 2342 2351 2474 3368 3965 Size: 393 462 140 108 9 123 894 597 Cuts at: 3965 4752 5020 1239 Size: 787 268 1524 Fragments arranged by size: 1524 894 787 597 462 393 268 140 123 108 9 EcoNI CCTnn'n_nnAGG Cuts at: 2176 2176 Size: 5305 EcoO109I rG'GnC_Cy Cuts at: 2250 2257 2447 2454 2497 2593 2250 Size: 7 190 7 43 96 4962 Fragments arranged by size: 4962 190 96 43 7 7 EcoRI G'AATT_C Cuts at: 2614 2992 2614 Size: 378 4927 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2222 2222 Size: 5305 FspI TGC'GCA Cuts at: 3811 3811 Size: 5305 HaeII r_GCGC'y Cuts at: 1678 2287 4452 1678 Size: 609 2165 2531 Fragments arranged by size: 2531 2165 609 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3946 3978 4020 4052 1373 Size: 32 135 32 2374 32 42 32 2626 Fragments arranged by size: 2626 2374 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2649 2895 669 Size: 988 281 711 246 3079 Fragments arranged by size: 3079 988 711 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5305 HpaI GTT'AAC Cuts at: 2895 2895 Size: 5305 KpnI G_GTAC'C Cuts at: 2630 2630 Size: 5305 MfeI C'AATT_G Cuts at: 2904 2904 Size: 5305 MluI A'CGCG_T Cuts at: 5181 5181 Size: 5305 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4419 4603 1305 Size: 3114 184 2007 Fragments arranged by size: 3114 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3483 Fragments arranged by size: 3483 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 3263 3622 3781 518 Size: 648 870 1227 359 159 2042 Fragments arranged by size: 2042 1227 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2269 2342 2721 3398 4546 4791 1606 Size: 663 73 379 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 663 379 245 73 NcoI C'CATG_G Cuts at: 513 1065 2289 513 Size: 552 1224 3529 Fragments arranged by size: 3529 1224 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5305 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5305 NotI GC'GGCC_GC Cuts at: 2752 2752 Size: 5305 NspI r_CATG'y Cuts at: 1132 1229 2663 4208 1132 Size: 97 1434 1545 2229 Fragments arranged by size: 2229 1545 1434 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5305 PciI A'CATG_T Cuts at: 4204 4204 Size: 5305 PflMI CCAn_nnn'nTGG Cuts at: 1108 2253 1108 Size: 1145 4160 PfoI T'CCnGG_A Cuts at: 2109 2490 2109 Size: 381 4924 PmeI GTTT'AAAC Cuts at: 2609 2609 Size: 5305 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3374 3406 4887 4919 3374 Size: 32 1481 32 3760 Fragments arranged by size: 3760 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2250 2257 2454 2593 2250 Size: 7 197 139 4962 Fragments arranged by size: 4962 197 139 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5305 PsiI TTA'TAA Cuts at: 2875 2875 Size: 5305 PspOMI G'GGCC_C Cuts at: 2447 2447 Size: 5305 PspXI vC'TCGA_Gb Cuts at: 2525 2525 Size: 5305 PstI C_TGCA'G Cuts at: 830 2169 2225 2274 2657 830 Size: 1339 56 49 383 3478 Fragments arranged by size: 3478 1339 383 56 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5305 PvuII CAG'CTG Cuts at: 2269 2269 Size: 5305 SalI G'TCGA_C Cuts at: 1936 2647 1936 Size: 711 4594 SbfI CC_TGCA'GG Cuts at: 2657 2657 Size: 5305 ScaI AGT'ACT Cuts at: 3553 5265 3553 Size: 1712 3593 SfcI C'TryA_G Cuts at: 826 1045 2165 2221 2270 2653 3788 4469 4660 Size: 219 1120 56 49 383 1135 681 191 Cuts at: 4660 826 Size: 1471 Fragments arranged by size: 1471 1135 1120 681 383 219 191 56 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5305 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5305 SmaI CCC'GGG Cuts at: 1872 2459 2632 1872 Size: 587 173 4545 Fragments arranged by size: 4545 587 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2525 3409 4310 4572 820 Size: 197 926 582 884 901 262 1553 Fragments arranged by size: 1553 926 901 884 582 262 197 SnaBI TAC'GTA Cuts at: 493 3096 493 Size: 2603 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5305 SphI G_CATG'C Cuts at: 1132 2663 1132 Size: 1531 3774 SspI AAT'ATT Cuts at: 5 52 3229 5 Size: 47 3177 2081 Fragments arranged by size: 3177 2081 47 StuI AGG'CCT Cuts at: 1517 2171 2531 1517 Size: 654 360 4291 Fragments arranged by size: 4291 654 360 StyI C'CwwG_G Cuts at: 513 1065 2289 2744 513 Size: 552 1224 455 3074 Fragments arranged by size: 3074 1224 552 455 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3332 3349 3502 3687 4132 325 Size: 1255 1752 17 153 185 445 1498 Fragments arranged by size: 1752 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3551 5263 96 Size: 274 80 33 51 1443 1574 1712 138 Fragments arranged by size: 1712 1574 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2210 2242 5265 5297 2210 Size: 32 3023 32 2218 Fragments arranged by size: 3023 2218 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5305 XbaI T'CTAG_A Cuts at: 2641 2641 Size: 5305 XhoI C'TCGA_G Cuts at: 1943 2525 1943 Size: 582 4723 XmaI C'CCGG_G Cuts at: 1870 2457 2630 1870 Size: 587 173 4545 Fragments arranged by size: 4545 587 173 XmnI GAAnn'nnTTC Cuts at: 3434 3434 Size: 5305 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2368 3113 276 Size: 53 83 186 936 834 745 2468 Fragments arranged by size: 2468 936 834 745 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI