################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 62 GAATTC [GA]^AATT[CT] AcyI 310 GACGCC G[GA]^CG[CT]C AfaI 318 GTAC GT^AC AflIII 167 ACACGT A^C[GA][CT]GT AluI 386 AGCT AG^CT AlwI 401 GGATC GGATC(4/5) ApoI 62 GAATTC [GA]^AATT[CT] AspHI 265 GTGCTC G[AT]GC[AT]^C AvaI 31 CCCGAG C^[CT]CG[GA]G BanII 129 GGGCTC G[GA]GC[CT]^C BbsI 181 GTCTTC GAAGAC(2/6) BpuAI 181 GTCTTC GAAGAC(2/6) BsaHI 310 GACGCC G[GA]^CG[CT]C BsgI 382 GTGCAG GTGCAG(16/14) BsiHKAI 265 GTGCTC G[AT]GC[AT]^C BsoBI 31 CCCGAG C^[CT]CG[GA]G BsrI 315 CCAGT ACTGG(1/-1) BssHII 349 GCGCGC G^CGCGC BstF5I 39 CATCC GGATG(2/0) BstYI 401 GGATCT [GA]^GATC[CT] DdeI 58 CTGAG C^T[ACGT]AG DpnII 402 GATC ^GATC DsaI 299 CCGTGG C^C[GA][CT]GG EcoNI 360 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 62 GAATTC G^AATTC FokI 39 CATCC GGATG(9/13) Hin1I 310 GACGCC G[GA]^CG[CT]C HinfI 391 GACTC G^A[ACGT]TC MboI 402 GATC ^GATC MflI 401 GGATCT [GA]^GATC[CT] NdeII 402 GATC ^GATC NlaIII 100 CATG CATG^ PleI 391 GACTC GAGTC(4/5) PvuII 385 CAGCTG CAG^CTG RsaI 318 GTAC GT^AC Sau3AI 402 GATC ^GATC TaqI 260 TCGA T^CGA Tsp509I 63 AATT ^AATT TthHB8I 260 TCGA T^CGA XhoII 401 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BcnI 158 CCCGG CC^[GC]GG 229 CCGGG BmyI 129 GGGCTC G[AGT]GC[ACT]^C 265 GTGCTC BseRI 121 GAGGAG GAGGAG(10/8) 217 GAGGAG Bsp1286I 129 GGGCTC G[AGT]GC[ACT]^C 265 GTGCTC BspMI 107 ACCTGC ACCTGC(4/8) 92 GCAGGT Cfr13I 210 GGGCC G^G[ACGT]CC 226 GGGCC HapII 159 CCGG C^CGG 229 CCGG HgaI 310 GACGC GACGC(5/10) 277 GCGTC HpaII 159 CCGG C^CGG 229 CCGG MboII 182 TCTTC GAAGA(8/7) 404 TCTTC MspA1I 343 CCGCTG C[AC]G^C[GT]G 385 CAGCTG MspI 159 CCGG C^CGG 229 CCGG NciI 158 CCCGG CC^[GC]GG 229 CCGGG Sau96I 210 GGGCC G^G[ACGT]CC 226 GGGCC SfaNI 136 GATGC GCATC(5/9) 38 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AccII AciI BbvI BsaJI BsiYI BslI BsmFI BssKI BstNI BstUI Cac8I CfoI EcoRII FauI Fnu4HI HaeIII HhaI HinP1I ItaI MaeII MnlI MvaI MvnI MwoI NlaIV ScrFI TseI