################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MroI MseI MslI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 412 GTCTAC GT^[AC][GT]AC AccII 361 CGCG CG^CG AcyI 346 GGCGCC G[GA]^CG[CT]C AlwI 183 GGATC GGATC(4/5) AspHI 228 GTGCTC G[AT]GC[AT]^C AvaI 257 CCCGGG C^[CT]CG[GA]G BalI 393 TGGCCA TGG^CCA BbeI 346 GGCGCC GGCGC^C BbsI 471 GAAGAC GAAGAC(2/6) BbvI 194 GCAGC GCAGC(8/12) BcgI 373 CGACGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 87 GCTGAGC GC^T[ACGT]AGC Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC BpuAI 471 GAAGAC GAAGAC(2/6) BsaHI 346 GGCGCC G[GA]^CG[CT]C BsiHKAI 228 GTGCTC G[AT]GC[AT]^C BsoBI 257 CCCGGG C^[CT]CG[GA]G BspMI 514 GCAGGT ACCTGC(4/8) BsrFI 123 GCCGGT [GA]^CCGG[CT] BssHII 360 GCGCGC G^CGCGC BstF5I 315 CATCC GGATG(2/0) BstUI 361 CGCG CG^CG CelII 87 GCTGAGC GC^T[ACGT]AGC Cfr10I 123 GCCGGT [GA]^CCGG[CT] DpnII 184 GATC ^GATC EaeI 393 TGGCCA [CT]^GGCC[GA] EcoT14I 179 CCAAGG C^C[AT][AT]GG FauI 321 GCGGG CCCGC(4/6) FokI 315 CATCC GGATG(9/13) HgaI 374 GACGC GACGC(5/10) Hin1I 346 GGCGCC G[GA]^CG[CT]C KasI 346 GGCGCC G^GCGCC MboI 184 GATC ^GATC MboII 471 GAAGA GAAGA(8/7) MluNI 393 TGGCCA TGG^CCA MscI 393 TGGCCA TGG^CCA MvnI 361 CGCG CG^CG NarI 346 GGCGCC GG^CGCC NdeII 184 GATC ^GATC NlaIII 567 CATG CATG^ PflMI 385 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 184 GATC ^GATC SexAI 44 ACCTGGT A^CC[AT]GGT SfaNI 144 GATGC GCATC(5/9) SfcI 414 CTACAG C^T[GA][CT]AG SfoI 346 GGCGCC GGC^GCC SmaI 257 CCCGGG CCC^GGG StyI 179 CCAAGG C^C[AT][AT]GG TseI 194 GCAGC G^C[AT]GC Van91I 385 CCACTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 257 CCCGGG C^CCGGG XmaI 257 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AflIII 329 ACGTGT A^C[GA][CT]GT 352 ACACGT AluI 213 AGCT AG^CT 449 AGCT BanII 53 GGGCTC G[GA]GC[CT]^C 90 GAGCCC BbrPI 277 CACGTG CAC^GTG 353 CACGTG Fnu4HI 194 GCAGC GC^[ACGT]GC 344 GCGGC HaeII 150 AGCGCC [GA]GCGC^[CT] 346 GGCGCC ItaI 194 GCAGC GC^[ACGT]GC 344 GCGGC MwoI 230 GCTCTTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 450 GCTGGTCCGGC PmaCI 277 CACGTG CAC^GTG 353 CACGTG PmlI 277 CACGTG CAC^GTG 353 CACGTG TspRI 167 CAGTG CA[GC]TG[ACGT][ACGT]^ 340 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AvaII BanI BcnI BmyI BpmI BsaAI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII HaeIII HapII HhaI HinP1I HinfI HpaII MaeII MnlI MspI MvaI NciI NlaIV PleI PpuMI Sau96I ScrFI