Restriction map for
pFN21AE5266
(Linear) (Six-base) MAPSORT of: KIEE5266.seq Check: 6785 from: 1 to: 576
KIEE5266.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AccI GT'mk_AC
Cuts at: 0 413 576
Size: 413 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 576
Size: 304 272
AflIII A'CryG_T
Cuts at: 0 329 352 576
Size: 329 23 224
Fragments arranged by size:
329 224 23
AvaI C'yCGr_G
Cuts at: 0 257 576
Size: 257 319
BanI G'GyrC_C
Cuts at: 0 162 346 526 576
Size: 162 184 180 50
Fragments arranged by size:
184 180 162 50
BanII G_rGCy'C
Cuts at: 0 57 94 576
Size: 57 37 482
Fragments arranged by size:
482 57 37
BbeI G_GCGC'C
Cuts at: 0 350 576
Size: 350 226
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 576
Size: 478 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 396 576
Size: 362 34 180
Fragments arranged by size:
362 180 34
BlpI GC'TnA_GC
Cuts at: 0 88 576
Size: 88 488
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 576
Size: 333 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 157 220 576
Size: 139 18 63 356
Fragments arranged by size:
356 139 63 18
BsaAI yAC'GTr
Cuts at: 0 279 330 355 576
Size: 279 51 25 221
Fragments arranged by size:
279 221 51 25
BsaHI Gr'CG_yC
Cuts at: 0 347 576
Size: 347 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 576
Size: 112 30 434
Fragments arranged by size:
434 112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 512 515 518 521 524 576
Size: 512 3 3 3 3 52
Fragments arranged by size:
512 52 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 95 576
Size: 95 481
BsiHKAI G_wGCw'C
Cuts at: 0 232 576
Size: 232 344
Bsp1286I G_dGCh'C
Cuts at: 0 57 94 232 576
Size: 57 37 138 344
Fragments arranged by size:
344 138 57 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 505 576
Size: 505 71
BsrFI r'CCGG_y
Cuts at: 0 123 576
Size: 123 453
BssHII G'CGCG_C
Cuts at: 0 360 576
Size: 360 216
EaeI y'GGCC_r
Cuts at: 0 393 576
Size: 393 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 157 220 304 576
Size: 139 18 63 84 272
Fragments arranged by size:
272 139 84 63 18
EcoO109I rG'GnC_Cy
Cuts at: 0 5 73 175 576
Size: 5 68 102 401
Fragments arranged by size:
401 102 68 5
HaeII r_GCGC'y
Cuts at: 0 154 350 576
Size: 154 196 226
Fragments arranged by size:
226 196 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 576
Size: 112 32 432
Fragments arranged by size:
432 112 32
KasI G'GCGC_C
Cuts at: 0 346 576
Size: 346 230
MscI TGG'CCA
Cuts at: 0 395 576
Size: 395 181
NarI GG'CG_CC
Cuts at: 0 347 576
Size: 347 229
PflMI CCAn_nnn'nTGG
Cuts at: 0 391 576
Size: 391 185
PmlI CAC'GTG
Cuts at: 0 279 355 576
Size: 279 76 221
Fragments arranged by size:
279 221 76
PpuMI rG'GwC_Cy
Cuts at: 0 5 73 175 576
Size: 5 68 102 401
Fragments arranged by size:
401 102 68 5
SanDI GG'GwC_CC
Cuts at: 0 175 576
Size: 175 401
SexAI A'CCwGG_T
Cuts at: 0 44 576
Size: 44 532
SfcI C'TryA_G
Cuts at: 0 414 576
Size: 414 162
SfoI GGC'GCC
Cuts at: 0 348 576
Size: 348 228
SmaI CCC'GGG
Cuts at: 0 259 576
Size: 259 317
StyI C'CwwG_G
Cuts at: 0 179 576
Size: 179 397
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 576
Size: 316 32 228
Fragments arranged by size:
316 228 32
XmaI C'CCGG_G
Cuts at: 0 257 576
Size: 257 319
Enzymes that do cut:
AccI AcuI AflIII AvaI BanI BanII BbeI BbsI
BcgI BlpI BmrI BpmI BsaAI BsaHI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrFI BssHII EaeI Eco57MI
EcoO109I HaeII Hin4I KasI MscI NarI PflMI PmlI
PpuMI SanDI SexAI SfcI SfoI SmaI StyI TstI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmnI ZraI