################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeIII MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp45I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AccI 1363 GTCTAC GT^[AC][GT]AC AflIII 854 ACACGT A^C[GA][CT]GT AspHI 1468 GTGCTC G[AT]GC[AT]^C BbrPI 622 CACGTG CAC^GTG BcgI 972 CGAAGGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 554 CTAG C^TAG BglI 1132 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1107 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 27 CGGCCG CG[GA][CT]^CG BsiHKAI 1468 GTGCTC G[AT]GC[AT]^C BsmAI 251 GTCTC GTCTC(1/5) BsrBI 131 GAGCGG CCGCTC(-3/-3) BsrFI 104 ACCGGC [GA]^CCGG[CT] Cfr10I 104 ACCGGC [GA]^CCGG[CT] DraIII 622 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 27 CGGCCG C^GGCCG EclXI 27 CGGCCG C^GGCCG Eco52I 27 CGGCCG C^GGCCG MaeI 554 CTAG C^TAG MamI 1107 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PmaCI 622 CACGTG CAC^GTG PmlI 622 CACGTG CAC^GTG SexAI 572 ACCTGGT A^CC[AT]GGT SfiI 1131 GGCCAACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TfiI 543 GATTC G^A[AT]TC Tsp509I 685 AATT ^AATT ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 1015 GGCGCC G[GA]^CG[CT]C 1171 GGCGCC AlwNI 1123 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1420 CAGTGGCTG Asp700I 147 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1323 GAAGCTGTTC AvaI 293 CCCGGG C^[CT]CG[GA]G 421 CCCGGG BalI 1130 TGGCCA TGG^CCA 1313 TGGCCA BbeI 1015 GGCGCC GGCGC^C 1171 GGCGCC BbsI 402 GAAGAC GAAGAC(2/6) 865 GTCTTC BpuAI 402 GAAGAC GAAGAC(2/6) 865 GTCTTC BsaHI 1015 GGCGCC G[GA]^CG[CT]C 1171 GGCGCC BsoBI 293 CCCGGG C^[CT]CG[GA]G 421 CCCGGG BsrI 878 ACTGG ACTGG(1/-1) 654 CCAGT BstYI 366 GGATCT [GA]^GATC[CT] 872 AGATCC DsaI 30 CCGCGG C^C[GA][CT]GG 41 CCGCGG EcoT14I 362 CCAAGG C^C[AT][AT]GG 893 CCTTGG Hin1I 1015 GGCGCC G[GA]^CG[CT]C 1171 GGCGCC HincII 528 GTCAAC GT[CT]^[GA]AC 1597 GTCAAC HindII 528 GTCAAC GT[CT]^[GA]AC 1597 GTCAAC KasI 1015 GGCGCC G^GCGCC 1171 GGCGCC KspI 30 CCGCGG CCGC^GG 41 CCGCGG MflI 366 GGATCT [GA]^GATC[CT] 872 AGATCC MluNI 1130 TGGCCA TGG^CCA 1313 TGGCCA MscI 1130 TGGCCA TGG^CCA 1313 TGGCCA NarI 1015 GGCGCC GG^CGCC 1171 GGCGCC PflMI 102 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1431 CCAGCTCTTGG PleI 1567 GACTC GAGTC(4/5) 1156 GAGTC PvuII 142 CAGCTG CAG^CTG 1516 CAGCTG SacII 30 CCGCGG CCGC^GG 41 CCGCGG SfoI 1015 GGCGCC GGC^GCC 1171 GGCGCC SmaI 293 CCCGGG CCC^GGG 421 CCCGGG StuI 975 AGGCCT AGG^CCT 1584 AGGCCT StyI 362 CCAAGG C^C[AT][AT]GG 893 CCTTGG Van91I 102 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1431 CCAGCTCTTGG XhoII 366 GGATCT [GA]^GATC[CT] 872 AGATCC XmaCI 293 CCCGGG C^CCGGG 421 CCCGGG XmaI 293 CCCGGG C^CCGGG 421 CCCGGG XmnI 147 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1323 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I XcmI