################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbsI BbvCI BcgI BclI BfrI BglII BlnI BpuAI BsiWI BsmI Bsp1407I BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstYI BstZ17I Bsu36I DraI DraIII DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KpnI MfeI MflI MluI MseI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 1115 TCCGGA T^CCGGA AfaI 1502 GTAC GT^AC AflIII 1498 ACGTGT A^C[GA][CT]GT AlwI 336 GATCC GGATC(4/5) Aor51HI 1040 AGCGCT AGC^GCT AvaII 748 GGTCC G^G[AT]CC AviII 1068 TGCGCA TGC^GCA BbrPI 1497 CACGTG CAC^GTG BciVI 1049 GTATCC GTATCC(6/5) BglI 557 GCCTGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 771 GCTCAGC GC^T[ACGT]AGC Bpu1102I 771 GCTCAGC GC^T[ACGT]AGC BsaAI 1497 CACGTG [CT]AC^GT[GA] BsaBI 1362 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1115 TCCGGA [AT]^CCGG[AT] BseAI 1115 TCCGGA T^CCGGA BseRI 1479 CTCCTC GAGGAG(10/8) BsiEI 104 CGGCCG CG[GA][CT]^CG BsmBI 410 CGTCTC CGTCTC(1/5) BspDI 23 ATCGAT AT^CGAT BspEI 1115 TCCGGA T^CCGGA BspHI 1421 TCATGA T^CATGA BsrI 929 CCAGT ACTGG(1/-1) BstEII 318 GGTCACC G^GT[ACGT]ACC BstPI 318 GGTCACC G^GT[ACGT]ACC CelII 771 GCTCAGC GC^T[ACGT]AGC ClaI 23 ATCGAT AT^CGAT CpoI 747 CGGTCCG CG^G[AT]CCG EagI 104 CGGCCG C^GGCCG EarI 1321 CTCTTC CTCTTC(1/4) EclXI 104 CGGCCG C^GGCCG Eco47III 1040 AGCGCT AGC^GCT Eco52I 104 CGGCCG C^GGCCG EcoNI 1160 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 318 GGTCACC G^GT[ACGT]ACC EcoT14I 314 CCATGG C^C[AT][AT]GG FseI 101 GGCCGGCC GGCCGG^CC FspI 1068 TGCGCA TGC^GCA Ksp632I 1321 CTCTTC CTCTTC(1/4) MaeIII 319 GTCAC ^GT[ACGT]AC MamI 1362 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1115 TCCGGA T^CCGGA NcoI 314 CCATGG C^CATGG NruI 933 TCGCGA TCG^CGA PmaCI 1497 CACGTG CAC^GTG PmlI 1497 CACGTG CAC^GTG RcaI 1421 TCATGA T^CATGA RsaI 1502 GTAC GT^AC RsrII 747 CGGTCCG CG^G[AT]CCG StyI 314 CCATGG C^C[AT][AT]GG TfiI 1119 GAATC G^A[AT]TC Tsp45I 319 GTCAC ^GT[GC]AC Tsp509I 1270 AATT ^AATT ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### ApaI 159 GGGCCC GGGCC^C 237 GGGCCC BalI 185 TGGCCA TGG^CCA 971 TGGCCA BbeI 217 GGCGCC GGCGC^C 1433 GGCGCC BfaI 43 CTAG C^TAG 706 CTAG BsaI 1156 GAGACC GGTCTC(1/5) 1088 GGTCTC BsgI 379 CTGCAC GTGCAG(16/14) 883 CTGCAC BsrBI 457 CCGCTC CCGCTC(-3/-3) 937 GAGCGG HgaI 787 GACGC GACGC(5/10) 301 GCGTC KasI 217 GGCGCC G^GCGCC 1433 GGCGCC MaeI 43 CTAG C^TAG 706 CTAG MluNI 185 TGGCCA TGG^CCA 971 TGGCCA MscI 185 TGGCCA TGG^CCA 971 TGGCCA NaeI 102 GCCGGC GCC^GGC 106 GCCGGC NarI 217 GGCGCC GG^CGCC 1433 GGCGCC NgoMIV 102 GCCGGC G^CCGGC 106 GCCGGC NspI 395 GCATGT [GA]CATG^[CT] 1205 GCATGT PflMI 264 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1380 CCAGCAGCTGG PvuII 985 CAGCTG CAG^CTG 1384 CAGCTG SacI 364 GAGCTC GAGCT^C 1396 GAGCTC SfcI 630 CTACAG C^T[GA][CT]AG 1044 CTACAG SfoI 217 GGCGCC GGC^GCC 1433 GGCGCC SmaI 162 CCCGGG CCC^GGG 891 CCCGGG Van91I 264 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1380 CCAGCAGCTGG XmaCI 162 CCCGGG C^CCGGG 891 CCCGGG XmaI 162 CCCGGG C^CCGGG 891 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AspHI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI SacII Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I