(Circular) (Six-base) MAPSORT of: pFN21AE3424.seq Check: 4132 from: 1 to: 6217 pFN21AE3424 6217 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2866 2962 2866 Size: 96 6121 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3062 3173 4280 5664 5932 1632 Size: 1430 111 1107 1384 268 1917 Fragments arranged by size: 1917 1430 1384 1107 268 111 AfeI AGC'GCT Cuts at: 3047 3047 Size: 6217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 3503 5116 6093 1203 Size: 2300 1613 977 1327 Fragments arranged by size: 2300 1613 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2820 1184 Size: 1636 4581 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3185 3217 3185 Size: 32 6185 AlwNI CAG_nnn'CTG Cuts at: 2185 2275 2473 2563 2674 2740 3391 3960 5532 Size: 90 198 90 111 66 651 569 1572 Cuts at: 5532 2185 Size: 2870 Fragments arranged by size: 2870 1572 651 569 198 111 90 90 66 ApaI G_GGCC'C Cuts at: 2168 2246 2168 Size: 78 6139 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2167 2366 2896 3197 3542 1870 Size: 73 224 199 530 301 345 4545 Fragments arranged by size: 4545 530 345 301 224 199 73 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 2849 2929 3438 3538 Size: 325 217 627 435 627 80 509 100 Cuts at: 3538 4993 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 627 509 435 325 217 100 80 BanII G_rGCy'C Cuts at: 1391 2168 2246 2373 2616 2969 3198 3405 3485 Size: 777 78 127 243 353 229 207 80 Cuts at: 3485 3536 1391 Size: 51 4072 Fragments arranged by size: 4072 777 353 243 229 207 127 80 78 51 BbeI G_GCGC'C Cuts at: 2226 3442 2226 Size: 1216 5001 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3065 4109 5319 3065 Size: 1044 1210 3963 Fragments arranged by size: 3963 1210 1044 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2568 4828 Size: 107 122 71 631 605 337 559 2260 Cuts at: 4828 136 Size: 1525 Fragments arranged by size: 2260 1525 631 605 559 337 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2777 3634 2777 Size: 857 5360 Bme1580I G_kGCm'C Cuts at: 1790 2096 2168 2246 2282 2852 4278 5434 1790 Size: 306 72 78 36 570 1426 1156 2573 Fragments arranged by size: 2573 1426 1156 570 306 78 72 36 BmgBI CAC'GTC Cuts at: 1318 2982 1318 Size: 1664 4553 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2928 3948 4906 Size: 416 53 83 399 24 1511 1020 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1753 1511 1020 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 3419 3470 3481 4877 1239 Size: 1211 969 51 11 1396 2579 Fragments arranged by size: 2579 1396 1211 969 51 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3099 3155 4880 882 Size: 617 1600 56 1725 2219 Fragments arranged by size: 2219 1725 1600 617 56 BsaAI yAC'GTr Cuts at: 493 1204 3504 4008 493 Size: 711 2300 504 2702 Fragments arranged by size: 2702 2300 711 504 BsaBI GATnn'nnATC Cuts at: 3371 6211 3371 Size: 2840 3377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 2306 3439 4024 Size: 53 83 186 936 690 83 1133 585 Cuts at: 4024 4406 5041 275 Size: 382 635 1451 Fragments arranged by size: 1451 1133 936 690 635 585 382 186 83 83 53 BsaWI w'CCGG_w Cuts at: 3120 4650 5048 5322 5469 5880 3120 Size: 1530 398 274 147 411 3457 Fragments arranged by size: 3457 1530 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3185 3215 1178 Size: 30 165 30 1782 30 4180 Fragments arranged by size: 4180 1782 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3475 1186 Size: 2289 3928 BseYI C'CCAG_C Cuts at: 2198 2275 2698 3021 3384 3391 5420 5916 2198 Size: 77 423 323 363 7 2029 496 2499 Fragments arranged by size: 2499 2029 496 423 363 323 77 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 2873 1163 Size: 573 633 504 4507 Fragments arranged by size: 4507 633 573 504 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 3667 4428 5456 1540 Size: 275 186 111 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 111 BsiHKAI G_wGCw'C Cuts at: 2373 3405 3476 4278 4363 5434 2373 Size: 1032 71 802 85 1071 3156 Fragments arranged by size: 3156 1071 1032 802 85 71 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 BsmBI CGTCTCn'nnnn_ Cuts at: 2421 2421 Size: 6217 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2168 2246 2282 2373 2616 2852 Size: 399 306 72 78 36 91 243 236 Cuts at: 2852 2969 3198 3405 3476 3485 3536 4278 4363 Size: 117 229 207 71 9 51 742 85 Cuts at: 4363 5434 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 243 236 229 207 117 91 85 78 72 71 51 36 9 BspEI T'CCGG_A Cuts at: 3120 5880 3120 Size: 2760 3457 BspHI T'CATG_A Cuts at: 3426 4106 5836 3426 Size: 680 1730 3807 Fragments arranged by size: 3807 1730 680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2866 2962 3123 3572 844 Size: 2022 96 161 449 3489 Fragments arranged by size: 3489 2022 449 161 96 BsrBI CCG'CTC Cuts at: 1394 1823 2464 2944 4104 1394 Size: 429 641 480 1160 3507 Fragments arranged by size: 3507 1160 641 480 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2111 2361 2920 3090 4861 5048 Size: 110 297 4 250 559 170 1771 187 Cuts at: 5048 1700 Size: 2869 Fragments arranged by size: 2869 1771 559 297 250 187 170 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2185 2406 2740 3082 794 Size: 1391 221 334 342 3929 Fragments arranged by size: 3929 1391 342 334 221 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 2323 1184 Size: 1139 5078 BstXI CCAn_nnnn'nTGG Cuts at: 2698 3016 3135 2698 Size: 318 119 5780 Fragments arranged by size: 5780 318 119 BstYI r'GATC_y Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212 Size: 1575 367 385 17 1452 86 12 346 Cuts at: 6212 1972 Size: 1977 Fragments arranged by size: 1977 1575 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2218 2319 2818 2839 3134 513 Size: 552 1153 101 499 21 295 3596 Fragments arranged by size: 3596 1153 552 499 295 101 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2128 2802 3733 4527 4547 2128 Size: 674 931 794 20 3798 Fragments arranged by size: 3798 931 794 674 20 ClaI AT'CG_AT Cuts at: 1554 2029 1554 Size: 475 5742 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2190 2316 Size: 54 1425 50 275 18 279 81 126 Cuts at: 2316 2619 2685 2862 2945 2976 3664 4553 6182 Size: 303 66 177 83 31 688 889 1629 Cuts at: 6182 8 Size: 43 Fragments arranged by size: 1629 1425 889 688 303 279 275 177 126 83 81 66 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 3664 1537 Size: 275 297 1555 4090 Fragments arranged by size: 4090 1555 297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3332 4147 1353 Size: 1979 815 3423 Fragments arranged by size: 3423 1979 815 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2238 2409 2745 3205 4804 5178 Size: 1064 138 649 171 336 460 1599 374 Cuts at: 5178 5324 387 Size: 146 1280 Fragments arranged by size: 1599 1280 1064 649 460 374 336 171 146 138 EcoICRI GAG'CTC Cuts at: 2371 3403 2371 Size: 1032 5185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 3062 3173 3419 3470 3481 4280 Size: 393 818 612 111 246 51 11 799 Cuts at: 4280 4877 5664 5932 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 818 799 787 612 597 393 268 246 111 51 11 EcoNI CCTnn'n_nnAGG Cuts at: 3169 3169 Size: 6217 EcoO109I rG'GnC_Cy Cuts at: 2164 2242 2243 2164 Size: 78 1 6138 Fragments arranged by size: 6138 78 1 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FseI GG_CCGG'CC Cuts at: 2111 2111 Size: 6217 FspI TGC'GCA Cuts at: 3075 4723 3075 Size: 1648 4569 HaeII r_GCGC'y Cuts at: 1678 2226 2563 3049 3442 5364 1678 Size: 548 337 486 393 1922 2531 Fragments arranged by size: 2531 1922 548 486 393 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373 Size: 32 135 32 3286 32 42 32 2626 Fragments arranged by size: 3286 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2222 3438 2222 Size: 1216 5001 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2282 3135 3337 5331 5515 1305 Size: 977 853 202 1994 184 2007 Fragments arranged by size: 2007 1994 977 853 202 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 2978 10 Size: 54 1425 343 360 786 3249 Fragments arranged by size: 3249 1425 786 360 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2330 2399 2432 2594 2807 4175 4534 Size: 648 1164 69 33 162 213 1368 359 Cuts at: 4534 4693 518 Size: 159 2042 Fragments arranged by size: 2042 1368 1164 648 359 213 162 159 69 33 MspA1I CmG'CkG Cuts at: 1606 2220 2228 2291 2300 2383 2473 2580 2683 Size: 614 8 63 9 83 90 107 103 Cuts at: 2683 2698 2820 2841 2992 3035 3391 3633 4310 Size: 15 122 21 151 43 356 242 677 Cuts at: 4310 5458 5703 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 614 356 245 242 151 122 107 103 90 83 63 43 21 15 9 8 NaeI GCC'GGC Cuts at: 2109 2113 2109 Size: 4 6213 NarI GG'CG_CC Cuts at: 2223 3439 2223 Size: 1216 5001 NcoI C'CATG_G Cuts at: 513 1065 2319 513 Size: 552 1254 4411 Fragments arranged by size: 4411 1254 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2107 2111 2107 Size: 4 6213 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NruI TCG'CGA Cuts at: 2940 2940 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2404 3214 3575 5120 1132 Size: 97 1175 810 361 1545 2229 Fragments arranged by size: 2229 1545 1175 810 361 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 3391 1108 Size: 1167 1116 3934 Fragments arranged by size: 3934 1167 1116 PfoI T'CCnGG_A Cuts at: 2343 2343 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PmlI CAC'GTG Cuts at: 3504 3504 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3185 3217 4286 4318 5799 5831 3185 Size: 32 1069 32 1481 32 3571 Fragments arranged by size: 3571 1481 1069 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2164 2242 2164 Size: 78 6139 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2618 2650 2618 Size: 32 6185 PstI C_TGCA'G Cuts at: 830 3569 830 Size: 2739 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2992 3391 2992 Size: 399 5818 RsrII CG'GwC_CG Cuts at: 2753 2753 Size: 6217 SacI G_AGCT'C Cuts at: 2373 3405 2373 Size: 1032 5185 SacII CC_GC'GG Cuts at: 2221 2821 2842 2221 Size: 600 21 5596 Fragments arranged by size: 5596 600 21 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2635 3049 3565 4700 5381 5572 826 Size: 219 1590 414 516 1135 681 191 1471 Fragments arranged by size: 1590 1471 1135 681 516 414 219 191 SfoI GGC'GCC Cuts at: 2224 3440 2224 Size: 1216 5001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 2169 2898 3544 1872 Size: 297 729 646 4545 Fragments arranged by size: 4545 729 646 297 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 5222 5484 820 Size: 197 926 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SrfI GCCC'GGGC Cuts at: 2169 2169 Size: 6217 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2319 3656 513 Size: 552 1254 1337 3074 Fragments arranged by size: 3074 1337 1254 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2494 2947 4244 4261 4414 4599 5044 Size: 1255 914 453 1297 17 153 185 445 Cuts at: 5044 325 Size: 1498 Fragments arranged by size: 1498 1297 1255 914 453 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4463 6175 96 Size: 274 80 33 51 1443 2486 1712 138 Fragments arranged by size: 2486 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 2167 2896 3542 1870 Size: 297 729 646 4545 Fragments arranged by size: 4545 729 646 297 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NsiI PacI PpuMI PspXI SanDI SapI SexAI SfiI SwaI XcmI