(Linear) (Six-base) MAPSORT of: KIEE3424.seq Check: 7577 from: 1 to: 1512 KIEE3424.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 861 957 1512 Size: 861 96 555 Fragments arranged by size: 861 555 96 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1057 1168 1512 Size: 1057 111 344 Fragments arranged by size: 1057 344 111 AfeI AGC'GCT Cuts at: 0 1042 1512 Size: 1042 470 AflIII A'CryG_T Cuts at: 0 1498 1512 Size: 1498 14 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 815 1512 Size: 815 697 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1180 1212 1512 Size: 1180 32 300 Fragments arranged by size: 1180 300 32 AlwNI CAG_nnn'CTG Cuts at: 0 180 270 468 558 669 735 1386 1512 Size: 180 90 198 90 111 66 651 126 Fragments arranged by size: 651 198 180 126 111 90 90 66 ApaI G_GGCC'C Cuts at: 0 163 241 1512 Size: 163 78 1271 Fragments arranged by size: 1271 163 78 AvaI C'yCGr_G Cuts at: 0 162 361 891 1192 1512 Size: 162 199 530 301 320 Fragments arranged by size: 530 320 301 199 162 BanI G'GyrC_C Cuts at: 0 217 844 924 1433 1512 Size: 217 627 80 509 79 Fragments arranged by size: 627 509 217 80 79 BanII G_rGCy'C Cuts at: 0 163 241 368 611 964 1193 1400 1480 Size: 163 78 127 243 353 229 207 80 Cuts at: 1480 1512 Size: 32 Fragments arranged by size: 353 243 229 207 163 127 80 78 32 BbeI G_GCGC'C Cuts at: 0 221 1437 1512 Size: 221 1216 75 Fragments arranged by size: 1216 221 75 BciVI GTATCCnnnnn_n' Cuts at: 0 1060 1512 Size: 1060 452 BglI GCCn_nnn'nGGC Cuts at: 0 563 1512 Size: 563 949 BlpI GC'TnA_GC Cuts at: 0 772 1512 Size: 772 740 Bme1580I G_kGCm'C Cuts at: 0 91 163 241 277 847 1512 Size: 91 72 78 36 570 665 Fragments arranged by size: 665 570 91 78 72 36 BmgBI CAC'GTC Cuts at: 0 977 1512 Size: 977 535 BmrI ACTGGGnnnn_n' Cuts at: 0 923 1512 Size: 923 589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 1414 1465 1476 1512 Size: 445 969 51 11 36 Fragments arranged by size: 969 445 51 36 11 BsaI GGTCTCn'nnnn_ Cuts at: 0 1094 1150 1512 Size: 1094 56 362 Fragments arranged by size: 1094 362 56 BsaAI yAC'GTr Cuts at: 0 1499 1512 Size: 1499 13 BsaBI GATnn'nnATC Cuts at: 0 1366 1512 Size: 1366 146 BsaHI Gr'CG_yC Cuts at: 0 218 301 1434 1512 Size: 218 83 1133 78 Fragments arranged by size: 1133 218 83 78 BsaWI w'CCGG_w Cuts at: 0 1115 1512 Size: 1115 397 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1180 1210 1512 Size: 1180 30 302 Fragments arranged by size: 1180 302 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1470 1512 Size: 1470 42 BseYI C'CCAG_C Cuts at: 0 193 270 693 1016 1379 1386 1512 Size: 193 77 423 323 363 7 126 Fragments arranged by size: 423 363 323 193 126 77 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 868 1512 Size: 364 504 644 Fragments arranged by size: 644 504 364 BsiEI CG_ry'CG Cuts at: 0 107 1512 Size: 107 1405 BsiHKAI G_wGCw'C Cuts at: 0 368 1400 1471 1512 Size: 368 1032 71 41 Fragments arranged by size: 1032 368 71 41 BsmBI CGTCTCn'nnnn_ Cuts at: 0 416 1512 Size: 416 1096 Bsp1286I G_dGCh'C Cuts at: 0 91 163 241 277 368 611 847 964 Size: 91 72 78 36 91 243 236 117 Cuts at: 964 1193 1400 1471 1480 1512 Size: 229 207 71 9 32 Fragments arranged by size: 243 236 229 207 117 91 91 78 72 71 36 32 9 BspEI T'CCGG_A Cuts at: 0 1115 1512 Size: 1115 397 BspHI T'CATG_A Cuts at: 0 1421 1512 Size: 1421 91 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 861 957 1118 1512 Size: 861 96 161 394 Fragments arranged by size: 861 394 161 96 BsrBI CCG'CTC Cuts at: 0 459 939 1512 Size: 459 480 573 Fragments arranged by size: 573 480 459 BsrFI r'CCGG_y Cuts at: 0 102 106 356 915 1085 1512 Size: 102 4 250 559 170 427 Fragments arranged by size: 559 427 250 170 102 4 BstAPI GCAn_nnn'nTGC Cuts at: 0 180 401 735 1077 1512 Size: 180 221 334 342 435 Fragments arranged by size: 435 342 334 221 180 BstEII G'GTnAC_C Cuts at: 0 318 1512 Size: 318 1194 BstXI CCAn_nnnn'nTGG Cuts at: 0 693 1011 1130 1512 Size: 693 318 119 382 Fragments arranged by size: 693 382 318 119 BtgI C'CryG_G Cuts at: 0 213 314 813 834 1129 1512 Size: 213 101 499 21 295 383 Fragments arranged by size: 499 383 295 213 101 21 BtsI GCAGTG_nn' Cuts at: 0 123 797 1512 Size: 123 674 715 Fragments arranged by size: 715 674 123 ClaI AT'CG_AT Cuts at: 0 24 1512 Size: 24 1488 EaeI y'GGCC_r Cuts at: 0 104 185 311 614 680 857 940 971 Size: 104 81 126 303 66 177 83 31 Cuts at: 971 1512 Size: 541 Fragments arranged by size: 541 303 177 126 104 83 81 66 31 EagI C'GGCC_G Cuts at: 0 104 1512 Size: 104 1408 EarI CTCTTCn'nnn_ Cuts at: 0 1327 1512 Size: 1327 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 233 404 740 1200 1512 Size: 233 171 336 460 312 Fragments arranged by size: 460 336 312 233 171 EcoICRI GAG'CTC Cuts at: 0 366 1398 1512 Size: 366 1032 114 Fragments arranged by size: 1032 366 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 1057 1168 1414 1465 1476 1512 Size: 445 612 111 246 51 11 36 Fragments arranged by size: 612 445 246 111 51 36 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 1164 1512 Size: 1164 348 EcoO109I rG'GnC_Cy Cuts at: 0 159 237 238 1512 Size: 159 78 1 1274 Fragments arranged by size: 1274 159 78 1 FseI GG_CCGG'CC Cuts at: 0 106 1512 Size: 106 1406 FspI TGC'GCA Cuts at: 0 1070 1512 Size: 1070 442 HaeII r_GCGC'y Cuts at: 0 221 558 1044 1437 1512 Size: 221 337 486 393 75 Fragments arranged by size: 486 393 337 221 75 KasI G'GCGC_C Cuts at: 0 217 1433 1512 Size: 217 1216 79 Fragments arranged by size: 1216 217 79 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 277 1130 1332 1512 Size: 277 853 202 180 Fragments arranged by size: 853 277 202 180 MscI TGG'CCA Cuts at: 0 187 973 1512 Size: 187 786 539 Fragments arranged by size: 786 539 187 MslI CAynn'nnrTG Cuts at: 0 325 394 427 589 802 1512 Size: 325 69 33 162 213 710 Fragments arranged by size: 710 325 213 162 69 33 MspA1I CmG'CkG Cuts at: 0 215 223 286 295 378 468 575 678 Size: 215 8 63 9 83 90 107 103 Cuts at: 678 693 815 836 987 1030 1386 1512 Size: 15 122 21 151 43 356 126 Fragments arranged by size: 356 215 151 126 122 107 103 90 83 63 43 21 15 9 8 NaeI GCC'GGC Cuts at: 0 104 108 1512 Size: 104 4 1404 Fragments arranged by size: 1404 104 4 NarI GG'CG_CC Cuts at: 0 218 1434 1512 Size: 218 1216 78 Fragments arranged by size: 1216 218 78 NcoI C'CATG_G Cuts at: 0 314 1512 Size: 314 1198 NgoMIV G'CCGG_C Cuts at: 0 102 106 1512 Size: 102 4 1406 Fragments arranged by size: 1406 102 4 NruI TCG'CGA Cuts at: 0 935 1512 Size: 935 577 NspI r_CATG'y Cuts at: 0 399 1209 1512 Size: 399 810 303 Fragments arranged by size: 810 399 303 PflMI CCAn_nnn'nTGG Cuts at: 0 270 1386 1512 Size: 270 1116 126 Fragments arranged by size: 1116 270 126 PfoI T'CCnGG_A Cuts at: 0 338 1512 Size: 338 1174 PmlI CAC'GTG Cuts at: 0 1499 1512 Size: 1499 13 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1180 1212 1512 Size: 1180 32 300 Fragments arranged by size: 1180 300 32 PspOMI G'GGCC_C Cuts at: 0 159 237 1512 Size: 159 78 1275 Fragments arranged by size: 1275 159 78 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 613 645 1512 Size: 613 32 867 Fragments arranged by size: 867 613 32 PvuII CAG'CTG Cuts at: 0 987 1386 1512 Size: 987 399 126 Fragments arranged by size: 987 399 126 RsrII CG'GwC_CG Cuts at: 0 748 1512 Size: 748 764 SacI G_AGCT'C Cuts at: 0 368 1400 1512 Size: 368 1032 112 Fragments arranged by size: 1032 368 112 SacII CC_GC'GG Cuts at: 0 216 816 837 1512 Size: 216 600 21 675 Fragments arranged by size: 675 600 216 21 SfcI C'TryA_G Cuts at: 0 630 1044 1512 Size: 630 414 468 Fragments arranged by size: 630 468 414 SfoI GGC'GCC Cuts at: 0 219 1435 1512 Size: 219 1216 77 Fragments arranged by size: 1216 219 77 SmaI CCC'GGG Cuts at: 0 164 893 1512 Size: 164 729 619 Fragments arranged by size: 729 619 164 SrfI GCCC'GGGC Cuts at: 0 164 1512 Size: 164 1348 StyI C'CwwG_G Cuts at: 0 314 1512 Size: 314 1198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 489 942 1512 Size: 489 453 570 Fragments arranged by size: 570 489 453 XmaI C'CCGG_G Cuts at: 0 162 891 1512 Size: 162 729 621 Fragments arranged by size: 729 621 162 Enzymes that do cut: AarI AcuI AfeI AflIII AlfI AloI AlwNI ApaI AvaI BanI BanII BbeI BciVI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BtgI BtsI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PflMI PfoI PmlI PpiI PspOMI PsrI PvuII RsrII SacI SacII SfcI SfoI SmaI SrfI StyI TaqII XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI BglII BmtI BplI Bpu10I BpuEI BsiWI BsmI BsrDI BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NsiI PacI PasI PciI PmeI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI