################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BciVI BcnI BfrI BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NarI NciI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AccI 754 GTCTAC GT^[AC][GT]AC AciI 16 CCGC CCGC(-3/-1) AcyI 946 GGCGTC G[GA]^CG[CT]C AflIII 489 ACATGT A^C[GA][CT]GT Aor51HI 914 AGCGCT AGC^GCT AvaI 923 CTCGGG C^[CT]CG[GA]G AvaII 376 GGACC G^G[AT]CC BanII 673 GAGCCC G[GA]GC[CT]^C BbrPI 435 CACGTG CAC^GTG BbsI 396 GTCTTC GAAGAC(2/6) BclI 503 TGATCA T^GATCA BglII 76 AGATCT A^GATCT BmyI 673 GAGCCC G[AGT]GC[ACT]^C BpuAI 396 GTCTTC GAAGAC(2/6) BsaAI 435 CACGTG [CT]AC^GT[GA] BsaHI 946 GGCGTC G[GA]^CG[CT]C BsaJI 146 CCTGGG C^C[ACGT][ACGT]GG BsgI 988 CTGCAC GTGCAG(16/14) BsmAI 189 GTCTC GTCTC(1/5) BsmFI 274 GGGAC GGGAC(10/14) BsmI 21 GAATGC GAATGC(1/-1) BsoBI 923 CTCGGG C^[CT]CG[GA]G Bsp1286I 673 GAGCCC G[AGT]GC[ACT]^C BspLU11I 489 ACATGT A^CATGT BsrDI 358 GCAATG GCAATG(2/0) BsrFI 943 GCCGGC [GA]^CCGG[CT] BstYI 76 AGATCT [GA]^GATC[CT] CfoI 915 GCGC GCG^C Cfr10I 943 GCCGGC [GA]^CCGG[CT] Cfr13I 376 GGACC G^G[ACGT]CC DraI 514 TTTAAA TTT^AAA EarI 718 GAAGAG CTCTTC(1/4) Eco47III 914 AGCGCT AGC^GCT EcoT22I 1157 ATGCAT ATGCA^T FbaI 503 TGATCA T^GATCA HaeII 914 AGCGCT [GA]GCGC^[CT] HapII 944 CCGG C^CGG HgaI 947 GCGTC GACGC(5/10) HhaI 915 GCGC GCG^C Hin1I 946 GGCGTC G[GA]^CG[CT]C HinP1I 915 GCGC G^CGC HpaII 944 CCGG C^CGG Ksp632I 718 GAAGAG CTCTTC(1/4) MaeII 436 ACGT A^CGT MfeI 1013 CAATTG C^AATTG MflI 76 AGATCT [GA]^GATC[CT] MspA1I 16 CCGCTG C[AC]G^C[GT]G MspI 944 CCGG C^CGG MunI 1013 CAATTG C^AATTG NaeI 943 GCCGGC GCC^GGC NgoMIV 943 GCCGGC G^CCGGC NlaIV 12 GGTTCC GG[ACGT]^[ACGT]CC NsiI 1157 ATGCAT ATGCA^T NspI 489 ACATGT [GA]CATG^[CT] PleI 664 GACTC GAGTC(4/5) PmaCI 435 CACGTG CAC^GTG PmlI 435 CACGTG CAC^GTG PstI 701 CTGCAG CTGCA^G Sau96I 376 GGACC G^G[ACGT]CC ScaI 1054 AGTACT AGT^ACT SfcI 701 CTGCAG C^T[GA][CT]AG StuI 462 AGGCCT AGG^CCT TaqI 639 TCGA T^CGA TspRI 263 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 639 TCGA T^CGA XhoII 76 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 238 AAATTT [GA]^AATT[CT] 1199 GAATTT AfaI 769 GTAC GT^AC 1055 GTAC ApoI 238 AAATTT [GA]^AATT[CT] 1199 GAATTT BbvI 696 GCAGC GCAGC(8/12) 1021 GCTGC BglI 952 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1168 GCCATGCTGGC BseRI 616 GAGGAG GAGGAG(10/8) 928 GAGGAG BsrI 264 ACTGG ACTGG(1/-1) 822 CCAGT BstAPI 1012 GCAATTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1246 GCACTTACTGC EaeI 266 TGGCCG [CT]^GGCC[GA] 819 CGGCCA Fnu4HI 696 GCAGC GC^[ACGT]GC 1021 GCTGC ItaI 696 GCAGC GC^[ACGT]GC 1021 GCTGC RsaI 769 GTAC GT^AC 1055 GTAC TseI 696 GCAGC G^C[AT]GC 1021 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AluI BfaI BspHI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII RcaI Sau3AI ScrFI SfaNI TfiI Tru9I Tsp45I Tsp509I