(Circular) (Six-base) MAPSORT of: pFN21AE6366.seq Check: 4639 from: 1 to: 6004 pFN21AE6366 6004 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 3347 1937 Size: 823 587 4594 Fragments arranged by size: 4594 823 587 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2670 3057 4067 5451 5719 1632 Size: 1038 387 1010 1384 268 1917 Fragments arranged by size: 1917 1384 1038 1010 387 268 AfeI AGC'GCT Cuts at: 2921 2921 Size: 6004 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 2494 4903 5880 1203 Size: 1291 2409 977 1327 Fragments arranged by size: 2409 1327 1291 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 3747 5319 3747 Size: 1572 4432 ApaLI G'TGCA_C Cuts at: 4061 5217 4061 Size: 1156 4848 ApoI r'AATT_y Cuts at: 1493 2243 3204 3313 3539 3691 5800 1493 Size: 750 961 109 226 152 2109 1697 Fragments arranged by size: 2109 1697 961 750 226 152 109 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2928 3329 1870 Size: 73 985 401 4545 Fragments arranged by size: 4545 985 401 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3325 4780 618 Size: 325 217 627 1538 1455 1842 Fragments arranged by size: 1842 1538 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2682 3323 1391 Size: 1291 641 4072 Fragments arranged by size: 4072 1291 641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 928 Size: 971 495 4538 Fragments arranged by size: 4538 971 495 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 2508 1548 Size: 960 5044 BfrBI ATG'CAT Cuts at: 3164 3164 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2963 3179 4615 Size: 107 122 71 631 605 1291 216 1436 Cuts at: 4615 136 Size: 1525 Fragments arranged by size: 1525 1436 1291 631 605 216 122 107 71 BglII A'GATC_T Cuts at: 2081 5999 2081 Size: 3918 2086 BlpI GC'TnA_GC Cuts at: 3421 3421 Size: 6004 Bme1580I G_kGCm'C Cuts at: 1790 4065 5221 1790 Size: 2275 1156 2573 Fragments arranged by size: 2573 2275 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3735 4693 442 Size: 416 53 83 399 24 2318 958 1753 Fragments arranged by size: 2318 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4664 1239 Size: 3425 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4667 882 Size: 617 3168 2219 Fragments arranged by size: 3168 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2442 3795 493 Size: 711 1238 1353 2702 Fragments arranged by size: 2702 1353 1238 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2952 3811 4193 4828 Size: 53 83 186 936 1419 859 382 635 Cuts at: 4828 275 Size: 1451 Fragments arranged by size: 1451 1419 936 859 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5779 Fragments arranged by size: 5779 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 2948 1186 Size: 1450 312 4242 Fragments arranged by size: 4242 1450 312 BseYI C'CCAG_C Cuts at: 2200 5207 5703 2200 Size: 3007 496 2501 Fragments arranged by size: 3007 2501 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2978 1163 Size: 573 1242 4189 Fragments arranged by size: 4189 1242 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4065 4150 5221 4065 Size: 85 1071 4848 Fragments arranged by size: 4848 1071 85 BsmI GAATG_Cn' Cuts at: 2032 3515 3608 2032 Size: 1483 93 4428 Fragments arranged by size: 4428 1483 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2682 3323 4065 4150 5221 1391 Size: 399 892 641 742 85 1071 2174 Fragments arranged by size: 2174 1071 892 742 641 399 85 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 2223 2730 2854 3082 3094 3893 5623 2223 Size: 507 124 228 12 799 1730 2604 Fragments arranged by size: 2604 1730 799 507 228 124 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3359 844 Size: 2515 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2370 4499 4673 65 Size: 1183 1122 2129 174 1396 Fragments arranged by size: 2129 1396 1183 1122 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2948 4648 4835 1700 Size: 110 1138 1700 187 2869 Fragments arranged by size: 2869 1700 1138 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 3023 3257 794 Size: 2229 234 3541 Fragments arranged by size: 3541 2229 234 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstYI r'GATC_y Cuts at: 1972 2081 3334 3701 4086 4103 5555 5641 5653 Size: 109 1253 367 385 17 1452 86 12 Cuts at: 5653 5999 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1253 385 367 346 109 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3021 487 Size: 683 56 1795 3470 Fragments arranged by size: 3470 1795 683 56 BtsI GCAGTG_nn' Cuts at: 3520 4314 4334 3520 Size: 794 20 5190 Fragments arranged by size: 5190 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 2521 3308 2521 Size: 787 5217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2824 3451 Size: 54 1425 50 275 18 441 553 627 Cuts at: 3451 4340 5969 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 627 553 441 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2718 3934 1353 Size: 1365 1216 3423 Fragments arranged by size: 3423 1365 1216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2670 3057 4067 4664 5451 5719 1239 Size: 393 1038 387 1010 597 787 268 1524 Fragments arranged by size: 1524 1038 1010 787 597 393 387 268 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 2923 5151 1678 Size: 1245 2228 2531 Fragments arranged by size: 2531 2228 1245 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 4645 4677 4719 Size: 32 135 32 494 32 2547 32 42 Cuts at: 4719 4751 1373 Size: 32 2626 Fragments arranged by size: 2626 2547 494 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3018 3603 3018 Size: 585 5419 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5118 5302 1305 Size: 3813 184 2007 Fragments arranged by size: 3813 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2100 2322 2349 2853 3962 4321 4480 Size: 648 934 222 27 504 1109 359 159 Cuts at: 4480 518 Size: 2042 Fragments arranged by size: 2042 1109 934 648 504 359 222 159 27 MspA1I CmG'CkG Cuts at: 1606 2023 3420 4097 5245 5490 1606 Size: 417 1397 677 1148 245 2120 Fragments arranged by size: 2120 1397 1148 677 417 245 NaeI GCC'GGC Cuts at: 2950 2950 Size: 6004 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NgoMIV G'CCGG_C Cuts at: 2948 2948 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NsiI A_TGCA'T Cuts at: 3166 3166 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2498 3362 4907 1132 Size: 97 1269 864 1545 2229 Fragments arranged by size: 2229 1545 1269 864 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 2494 4903 2494 Size: 2409 3595 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PmlI CAC'GTG Cuts at: 2442 2442 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2484 2516 4073 4105 5586 5618 2484 Size: 32 1557 32 1481 32 2870 Fragments arranged by size: 2870 1557 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3159 3574 3159 Size: 415 5589 PstI C_TGCA'G Cuts at: 830 2710 3356 830 Size: 1880 646 3478 Fragments arranged by size: 3478 1880 646 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 3061 4252 5964 3061 Size: 1191 1712 3101 Fragments arranged by size: 3101 1712 1191 SfcI C'TryA_G Cuts at: 826 1045 2706 3352 4487 5168 5359 826 Size: 219 1661 646 1135 681 191 1471 Fragments arranged by size: 1661 1471 1135 681 646 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 2469 1517 Size: 952 5052 StyI C'CwwG_G Cuts at: 513 1065 2004 3443 513 Size: 552 939 1439 3074 Fragments arranged by size: 3074 1439 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2972 4031 4048 4201 4386 4831 325 Size: 1255 1392 1059 17 153 185 445 1498 Fragments arranged by size: 1498 1392 1255 1059 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3059 4250 5962 Size: 274 80 33 51 1443 1082 1191 1712 Cuts at: 5962 96 Size: 138 Fragments arranged by size: 1712 1443 1191 1082 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2272 2304 5964 5996 2272 Size: 32 3660 32 2280 Fragments arranged by size: 3660 2280 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 4133 4133 Size: 6004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI