################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI ClaI CpoI DraI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO109I EcoO65I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Alw44I 241 GTGCAC G^TGCAC AlwI 258 GATCC GGATC(4/5) AocI 301 CCTGAGG CC^T[ACGT]AGG ApaLI 241 GTGCAC G^TGCAC AvaI 185 CTCGGG C^[CT]CG[GA]G AvaII 93 GGACC G^G[AT]CC BbvCI 614 CCTCAGC CCTCAGC(-5/-2) BcgI 208 CGAGAGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaI 978 GAGACC GGTCTC(1/5) BsgI 490 GTGCAG GTGCAG(16/14) BsmFI 531 GTCCC GGGAC(10/14) BsoBI 185 CTCGGG C^[CT]CG[GA]G BspHI 825 TCATGA T^CATGA BsrFI 200 GCCGGC [GA]^CCGG[CT] BstYI 257 AGATCC [GA]^GATC[CT] Bsu36I 301 CCTGAGG CC^T[ACGT]AGG Cfr10I 200 GCCGGC [GA]^CCGG[CT] DpnII 258 GATC ^GATC DsaI 800 CCACGG C^C[GA][CT]GG Eco81I 301 CCTGAGG CC^T[ACGT]AGG EcoNI 297 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 627 GATATC GAT^ATC EcoT22I 986 ATGCAT ATGCA^T HincII 880 GTTGAC GT[CT]^[GA]AC HindII 880 GTTGAC GT[CT]^[GA]AC MboI 258 GATC ^GATC MflI 257 AGATCC [GA]^GATC[CT] NaeI 200 GCCGGC GCC^GGC NdeII 258 GATC ^GATC NgoMIV 200 GCCGGC G^CCGGC NsiI 986 ATGCAT ATGCA^T PflMI 337 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 725 CTGCAG CTGCA^G RcaI 825 TCATGA T^CATGA Sau3AI 258 GATC ^GATC SfcI 725 CTGCAG C^T[GA][CT]AG Tsp45I 358 GTGAC ^GT[GC]AC Van91I 337 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 543 CCAGGAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 257 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 256 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 283 CAGTTCCTG Asp700I 49 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1173 GAAGGCCTTC BalI 290 TGGCCA TGG^CCA 420 TGGCCA BcnI 486 CCCGG CC^[GC]GG 634 CCGGG BfaI 58 CTAG C^TAG 887 CTAG BlpI 1190 GCTCAGC GC^T[ACGT]AGC 1358 GCTCAGC Bpu1102I 1190 GCTCAGC GC^T[ACGT]AGC 1358 GCTCAGC BstXI 14 CCACAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 96 CCAGGACTTTGG CelII 1190 GCTCAGC GC^T[ACGT]AGC 1358 GCTCAGC Cfr13I 93 GGACC G^G[ACGT]CC 181 GGCCC DrdI 522 GACTCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 764 GACATTTCAGTC EcoT14I 248 CCAAGG C^C[AT][AT]GG 397 CCAAGG HindIII 314 AAGCTT A^AGCTT 680 AAGCTT HphI 1057 GGTGA GGTGA(8/7) 1367 TCACC MaeI 58 CTAG C^TAG 887 CTAG MaeIII 164 GTTAC ^GT[ACGT]AC 358 GTGAC MluNI 290 TGGCCA TGG^CCA 420 TGGCCA MscI 290 TGGCCA TGG^CCA 420 TGGCCA MslI 826 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1051 CATAGTGGTG NciI 486 CCCGG CC^[GC]GG 634 CCGGG NlaIV 125 GGAGCC GG[ACGT]^[ACGT]CC 393 GGAGCC NspI 988 GCATGT [GA]CATG^[CT] 1249 GCATGT Sau96I 93 GGACC G^G[ACGT]CC 181 GGCCC StyI 248 CCAAGG C^C[AT][AT]GG 397 CCAAGG XmnI 49 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1173 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AluI ApoI AspHI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI EaeI EcoRI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIII PleI SacI ScrFI SfaNI StuI TfiI TseI Tsp509I TspRI