(Circular) (Six-base) MAPSORT of: pFN21AE3217.seq Check: 2247 from: 1 to: 6187 pFN21AE3217 6187 bp With 182 enzymes: SgfI * March 28, 2012 12:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2489 2700 3001 3085 3169 4250 5634 Size: 467 390 211 301 84 84 1081 1384 Cuts at: 5634 5902 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1081 467 390 301 268 211 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2802 2834 2802 Size: 32 6155 AlwNI CAG_nnn'CTG Cuts at: 2266 2293 3930 5502 2266 Size: 27 1637 1572 2951 Fragments arranged by size: 2951 1637 1572 27 ApaLI G'TGCA_C Cuts at: 2246 4244 5400 2246 Size: 1998 1156 3033 Fragments arranged by size: 3033 1998 1156 ApoI r'AATT_y Cuts at: 1493 2857 2967 3051 3219 3303 3387 3496 3722 Size: 1364 110 84 168 84 84 109 226 Cuts at: 3722 3874 5983 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 168 152 110 109 84 84 84 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2190 3512 1870 Size: 73 247 1322 4545 Fragments arranged by size: 4545 1322 247 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3508 4963 618 Size: 325 217 627 1721 1455 1842 Fragments arranged by size: 1842 1721 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2945 3029 3120 3197 3365 3506 1391 Size: 1554 84 91 77 168 141 4072 Fragments arranged by size: 4072 1554 168 141 91 84 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5216 BbvCI CC'TCA_GC Cuts at: 2620 2620 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2202 2236 4378 4412 1903 Size: 34 265 34 2142 34 3678 Fragments arranged by size: 3678 2142 265 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BfrBI ATG'CAT Cuts at: 2993 2993 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 3196 3364 3604 3196 Size: 168 240 5779 Fragments arranged by size: 5779 240 168 Bme1580I G_kGCm'C Cuts at: 1790 2250 4248 5404 1790 Size: 460 1998 1156 2573 Fragments arranged by size: 2573 1998 1156 460 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2266 2266 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2025 2149 2288 2393 2414 2673 2997 3165 Size: 786 124 139 105 21 259 324 168 Cuts at: 3165 3249 3333 3417 4847 1239 Size: 84 84 84 1430 2579 Fragments arranged by size: 2579 1430 786 324 259 168 139 124 105 84 84 84 21 Bpu10I CC'TnA_GC Cuts at: 2620 2620 Size: 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4312 5177 5475 4312 Size: 865 298 5024 Fragments arranged by size: 5024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2977 4850 882 Size: 617 1478 1873 2219 Fragments arranged by size: 2219 1873 1478 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 1178 Size: 30 165 30 759 30 5173 Fragments arranged by size: 5173 759 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 2390 2411 1186 Size: 1138 66 21 4962 Fragments arranged by size: 4962 1138 66 21 BseYI C'CCAG_C Cuts at: 3375 5390 5886 3375 Size: 2015 496 3676 Fragments arranged by size: 3676 2015 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2516 1163 Size: 573 780 4834 Fragments arranged by size: 4834 780 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 2467 2945 3029 3120 3197 3365 4248 4333 Size: 217 478 84 91 77 168 883 85 Cuts at: 4333 5404 2250 Size: 1071 3033 Fragments arranged by size: 3033 1071 883 478 217 168 91 85 84 77 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2467 2945 3029 3120 3197 3365 Size: 399 460 217 478 84 91 77 168 Cuts at: 3365 3506 4248 4333 5404 1391 Size: 141 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 460 399 217 168 141 91 85 84 77 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 2830 4076 5806 2830 Size: 1246 1730 3211 Fragments arranged by size: 3211 1730 1246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3542 844 Size: 2698 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 4856 65 Size: 1183 3434 174 1396 Fragments arranged by size: 3434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 4831 5018 1700 Size: 110 395 2626 187 2869 Fragments arranged by size: 2869 2626 395 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6187 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstXI CCAn_nnnn'nTGG Cuts at: 2026 2108 2026 Size: 82 6105 BstYI r'GATC_y Cuts at: 1972 2262 3517 3884 4269 4286 5738 5824 5836 Size: 290 1255 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1255 385 367 346 290 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2307 2307 Size: 6187 BtgI C'CryG_G Cuts at: 513 1065 2805 513 Size: 552 1740 3895 Fragments arranged by size: 3895 1740 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 3703 4497 4517 3703 Size: 794 20 5373 Fragments arranged by size: 5373 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2533 2775 5194 809 Size: 507 1217 242 2419 1802 Fragments arranged by size: 2419 1802 1217 507 242 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2339 2425 Size: 54 1425 50 275 18 465 44 86 Cuts at: 2425 3634 4523 6152 8 Size: 1209 889 1629 43 Fragments arranged by size: 1629 1425 1209 889 465 275 86 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4117 1353 Size: 2764 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2943 3027 3118 3195 3363 2943 Size: 84 91 77 168 5767 Fragments arranged by size: 5767 168 91 84 77 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2025 2099 2149 2288 2393 2414 2489 Size: 393 393 74 50 139 105 21 75 Cuts at: 2489 2673 2700 2997 3001 3085 3165 3169 3249 Size: 184 27 297 4 84 80 4 80 Cuts at: 3249 3333 3417 4250 4847 5634 5902 1239 Size: 84 84 833 597 787 268 1524 Fragments arranged by size: 1524 833 787 597 393 393 297 268 184 139 105 84 84 84 80 80 75 74 50 27 21 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2306 Size: 6187 EcoRI G'AATT_C Cuts at: 2857 2967 3051 3303 3387 3496 3874 2857 Size: 110 84 252 84 109 378 5170 Fragments arranged by size: 5170 378 252 110 109 84 84 EcoRV GAT'ATC Cuts at: 2634 2634 Size: 6187 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 5334 1678 Size: 3656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2512 2544 2944 2976 4828 Size: 32 135 32 940 32 400 32 1852 Cuts at: 4828 4860 4902 4934 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1852 940 400 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2887 3531 3777 669 Size: 988 281 949 644 246 3079 Fragments arranged by size: 3079 988 949 644 281 246 HindIII A'AGCT_T Cuts at: 748 2319 2685 748 Size: 1571 366 4250 Fragments arranged by size: 4250 1571 366 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 5301 5485 1305 Size: 957 3039 184 2007 Fragments arranged by size: 3039 2007 957 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 2427 10 Size: 54 1425 343 465 130 3770 Fragments arranged by size: 3770 1425 465 343 130 54 MslI CAynn'nnrTG Cuts at: 518 1166 2835 3060 4145 4504 4663 518 Size: 648 1669 225 1085 359 159 2042 Fragments arranged by size: 2042 1669 1085 648 359 225 159 MspA1I CmG'CkG Cuts at: 1606 3603 4280 5428 5673 1606 Size: 1997 677 1148 245 2120 Fragments arranged by size: 2120 1997 1148 677 245 NaeI GCC'GGC Cuts at: 2207 2207 Size: 6187 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5635 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NgoMIV G'CCGG_C Cuts at: 2205 2205 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NsiI A_TGCA'T Cuts at: 2995 2995 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2997 3258 3545 5090 1132 Size: 97 1768 261 287 1545 2229 Fragments arranged by size: 2229 1768 1545 287 261 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 2348 1108 Size: 1240 4947 PfoI T'CCnGG_A Cuts at: 2265 2547 2650 2265 Size: 282 103 5802 Fragments arranged by size: 5802 282 103 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 2734 3539 830 Size: 1904 805 3478 Fragments arranged by size: 3478 1904 805 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 SacI G_AGCT'C Cuts at: 2945 3029 3120 3197 3365 2945 Size: 84 91 77 168 5767 Fragments arranged by size: 5767 168 91 84 77 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2730 3535 4670 5351 5542 826 Size: 219 1685 805 1135 681 191 1471 Fragments arranged by size: 1685 1471 1135 805 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4291 5192 5454 820 Size: 197 926 2348 901 262 1553 Fragments arranged by size: 2348 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 2930 3182 3453 1517 Size: 1413 252 271 4251 Fragments arranged by size: 4251 1413 271 252 StyI C'CwwG_G Cuts at: 513 1065 2253 2402 3626 513 Size: 552 1188 149 1224 3074 Fragments arranged by size: 3074 1224 1188 552 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3389 4214 4231 4384 4569 5014 325 Size: 1255 1809 825 17 153 185 445 1498 Fragments arranged by size: 1809 1498 1255 825 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4433 6145 96 Size: 274 80 33 51 1443 2456 1712 138 Fragments arranged by size: 2456 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6147 6179 6147 Size: 32 6155 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2555 2555 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 2058 3182 4316 2058 Size: 1124 1134 3929 Fragments arranged by size: 3929 1134 1124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoO109I FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI