Restriction map for
pFN21AE3217
(Linear) (Six-base) MAPSORT of: KIEE3217.seq Check: 3220 from: 1 to: 1482
KIEE3217.orf
With 182 enzymes: SgfI *
March 22, 2012 16:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 484 695 996 1080 1164 1482
Size: 94 390 211 301 84 84 318
Fragments arranged by size:
390 318 301 211 94 84 84
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 797 829 1482
Size: 797 32 653
Fragments arranged by size:
797 653 32
AlwNI CAG_nnn'CTG
Cuts at: 0 261 288 1482
Size: 261 27 1194
Fragments arranged by size:
1194 261 27
ApaLI G'TGCA_C
Cuts at: 0 241 1482
Size: 241 1241
ApoI r'AATT_y
Cuts at: 0 852 962 1046 1214 1298 1382 1482
Size: 852 110 84 168 84 84 100
Fragments arranged by size:
852 168 110 100 84 84 84
AvaI C'yCGr_G
Cuts at: 0 185 1482
Size: 185 1297
BanII G_rGCy'C
Cuts at: 0 940 1024 1115 1192 1360 1482
Size: 940 84 91 77 168 122
Fragments arranged by size:
940 168 122 91 84 77
BbvCI CC'TCA_GC
Cuts at: 0 615 1482
Size: 615 867
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 197 231 1482
Size: 197 34 1251
Fragments arranged by size:
1251 197 34
BfrBI ATG'CAT
Cuts at: 0 988 1482
Size: 988 494
BlpI GC'TnA_GC
Cuts at: 0 1191 1359 1482
Size: 1191 168 123
Fragments arranged by size:
1191 168 123
Bme1580I G_kGCm'C
Cuts at: 0 245 1482
Size: 245 1237
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 1482
Size: 261 1221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 144 283 388 409 668 992 1160
Size: 20 124 139 105 21 259 324 168
Cuts at: 1160 1244 1328 1412 1482
Size: 84 84 84 70
Fragments arranged by size:
324 259 168 139 124 105 84 84
84 70 21 20
Bpu10I CC'TnA_GC
Cuts at: 0 615 1482
Size: 615 867
BsaI GGTCTCn'nnnn_
Cuts at: 0 972 1482
Size: 972 510
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 1482
Size: 157 30 1295
Fragments arranged by size:
1295 157 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 385 406 1482
Size: 319 66 21 1076
Fragments arranged by size:
1076 319 66 21
BseYI C'CCAG_C
Cuts at: 0 1370 1482
Size: 1370 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1482
Size: 511 971
BsiHKAI G_wGCw'C
Cuts at: 0 245 462 940 1024 1115 1192 1360 1482
Size: 245 217 478 84 91 77 168 122
Fragments arranged by size:
478 245 217 168 122 91 84 77
Bsp1286I G_dGCh'C
Cuts at: 0 245 462 940 1024 1115 1192 1360 1482
Size: 245 217 478 84 91 77 168 122
Fragments arranged by size:
478 245 217 168 122 91 84 77
BspHI T'CATG_A
Cuts at: 0 825 1482
Size: 825 657
BsrFI r'CCGG_y
Cuts at: 0 200 1482
Size: 200 1282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 103 1482
Size: 21 82 1379
Fragments arranged by size:
1379 82 21
BstYI r'GATC_y
Cuts at: 0 257 1482
Size: 257 1225
Bsu36I CC'TnA_GG
Cuts at: 0 302 1482
Size: 302 1180
BtgI C'CryG_G
Cuts at: 0 800 1482
Size: 800 682
DrdI GACnn_nn'nnGTC
Cuts at: 0 528 770 1482
Size: 528 242 712
Fragments arranged by size:
712 528 242
EaeI y'GGCC_r
Cuts at: 0 290 334 420 1482
Size: 290 44 86 1062
Fragments arranged by size:
1062 290 86 44
EcoICRI GAG'CTC
Cuts at: 0 938 1022 1113 1190 1358 1482
Size: 938 84 91 77 168 124
Fragments arranged by size:
938 168 124 91 84 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 94 144 283 388 409 484 668
Size: 20 74 50 139 105 21 75 184
Cuts at: 668 695 992 996 1080 1160 1164 1244 1328
Size: 27 297 4 84 80 4 80 84
Cuts at: 1328 1412 1482
Size: 84 70
Fragments arranged by size:
297 184 139 105 84 84 84 80
80 75 74 70 50 27 21 20
4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 1482
Size: 301 1181
EcoRI G'AATT_C
Cuts at: 0 852 962 1046 1298 1382 1482
Size: 852 110 84 252 84 100
Fragments arranged by size:
852 252 110 100 84 84
EcoRV GAT'ATC
Cuts at: 0 629 1482
Size: 629 853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 507 539 939 971 1482
Size: 507 32 400 32 511
Fragments arranged by size:
511 507 400 32 32
HincII GTy'rAC
Cuts at: 0 882 1482
Size: 882 600
HindIII A'AGCT_T
Cuts at: 0 314 680 1482
Size: 314 366 802
Fragments arranged by size:
802 366 314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1482
Size: 257 1225
MscI TGG'CCA
Cuts at: 0 292 422 1482
Size: 292 130 1060
Fragments arranged by size:
1060 292 130
MslI CAynn'nnrTG
Cuts at: 0 830 1055 1482
Size: 830 225 427
Fragments arranged by size:
830 427 225
NaeI GCC'GGC
Cuts at: 0 202 1482
Size: 202 1280
NgoMIV G'CCGG_C
Cuts at: 0 200 1482
Size: 200 1282
NsiI A_TGCA'T
Cuts at: 0 990 1482
Size: 990 492
NspI r_CATG'y
Cuts at: 0 992 1253 1482
Size: 992 261 229
Fragments arranged by size:
992 261 229
PflMI CCAn_nnn'nTGG
Cuts at: 0 343 1482
Size: 343 1139
PfoI T'CCnGG_A
Cuts at: 0 260 542 645 1482
Size: 260 282 103 837
Fragments arranged by size:
837 282 260 103
PstI C_TGCA'G
Cuts at: 0 729 1482
Size: 729 753
SacI G_AGCT'C
Cuts at: 0 940 1024 1115 1192 1360 1482
Size: 940 84 91 77 168 122
Fragments arranged by size:
940 168 122 91 84 77
SfcI C'TryA_G
Cuts at: 0 725 1482
Size: 725 757
StuI AGG'CCT
Cuts at: 0 925 1177 1448 1482
Size: 925 252 271 34
Fragments arranged by size:
925 271 252 34
StyI C'CwwG_G
Cuts at: 0 248 397 1482
Size: 248 149 1085
Fragments arranged by size:
1085 248 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1384 1482
Size: 1384 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 550 1482
Size: 550 932
XmnI GAAnn'nnTTC
Cuts at: 0 53 1177 1482
Size: 53 1124 305
Fragments arranged by size:
1124 305 53
Enzymes that do cut:
AcuI AloI AlwNI ApaLI ApoI AvaI BanII BbvCI
BcgI BfrBI BlpI Bme1580I BplI BpmI Bpu10I BsaI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrFI
BstXI BstYI Bsu36I BtgI DrdI EaeI EcoICRI Eco57MI
EcoNI EcoRI EcoRV Hin4I HincII HindIII MmeI MscI
MslI NaeI NgoMIV NsiI NspI PflMI PfoI PstI
SacI SfcI StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BciVI BclI
BglI BglII BmgBI BmrI BmtI BpuEI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EagI
EarI EciI EcoO109I FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MspA1I NarI NcoI
NdeI NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI ZraI