################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeII MamI MluI MluNI MroI MscI MseI MspA1I MvnI MwoI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 73 AAATTC [GA]^AATT[CT] AfaI 550 GTAC GT^AC ApoI 73 AAATTC [GA]^AATT[CT] AspHI 64 GAGCTC G[AT]GC[AT]^C AvaI 684 CTCGGG C^[CT]CG[GA]G AvaII 677 GGTCC G^G[AT]CC BanII 64 GAGCTC G[GA]GC[CT]^C BbvCI 672 GCTGAGG CCTCAGC(-5/-2) BglII 299 AGATCT A^GATCT BmyI 64 GAGCTC G[AGT]GC[ACT]^C BsaI 262 GAGACC GGTCTC(1/5) BsaWI 389 TCCGGT [AT]^CCGG[AT] BsiHKAI 64 GAGCTC G[AT]GC[AT]^C BsoBI 684 CTCGGG C^[CT]CG[GA]G Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C BspMI 715 ACCTGC ACCTGC(4/8) BsrFI 158 GCCGGC [GA]^CCGG[CT] BsrI 477 ACTGG ACTGG(1/-1) BstF5I 660 CATCC GGATG(2/0) BstXI 110 CCACCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 299 AGATCT [GA]^GATC[CT] Cfr10I 158 GCCGGC [GA]^CCGG[CT] DraII 676 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 676 AGGTCCC [GA]G^G[ACGT]CC[CT] FokI 660 CATCC GGATG(9/13) MfeI 415 CAATTG C^AATTG MflI 299 AGATCT [GA]^GATC[CT] MunI 415 CAATTG C^AATTG NaeI 158 GCCGGC GCC^GGC NgoMIV 158 GCCGGC G^CCGGC PflMI 312 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 676 AGGTCCC [GA]G^G[AT]CC[CT] RsaI 550 GTAC GT^AC SacI 64 GAGCTC GAGCT^C ScaI 549 AGTACT AGT^ACT Van91I 312 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 467 CCATTGTCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 299 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 440 CCGC BpmI 67 CTCCAG CTGGAG(16/14) 583 CTGGAG BsaJI 282 CCAGGG C^C[ACGT][ACGT]GG 443 CCTGGG BseRI 634 CTCCTC GAGGAG(10/8) 894 GAGGAG BsgI 564 CTGCAC GTGCAG(16/14) 717 CTGCAC BsmAI 262 GAGAC GTCTC(1/5) 555 GAGAC BsmFI 328 GGGAC GGGAC(10/14) 678 GTCCC Cac8I 158 GCCGGC GC[ACGT]^[ACGT]GC 562 GCCTGC HapII 159 CCGG C^CGG 390 CCGG HpaII 159 CCGG C^CGG 390 CCGG MspI 159 CCGG C^CGG 390 CCGG SfaNI 240 GATGC GCATC(5/9) 669 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI BbvI BfaI BsiYI BslI BspHI BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HphI ItaI MaeI MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV RcaI Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI