################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbvCI BciVI BclI BfrI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SwaI Tru9I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 112 CGCG CG^CG AcsI 1303 GAATTC [GA]^AATT[CT] AflIII 617 ACGTGT A^C[GA][CT]GT ApaI 1008 GGGCCC GGGCC^C ApoI 1303 GAATTC [GA]^AATT[CT] Asp700I 1050 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1066 GAGCTC G[AT]GC[AT]^C AvaI 763 CTCGAG C^[CT]CG[GA]G BamHI 909 GGATCC G^GATCC BanI 1487 GGCGCC G^G[CT][GA]CC BbeI 1487 GGCGCC GGCGC^C BbrPI 16 CACGTG CAC^GTG BfaI 344 CTAG C^TAG BglII 1082 AGATCT A^GATCT BlpI 589 GCTCAGC GC^T[ACGT]AGC Bpu1102I 589 GCTCAGC GC^T[ACGT]AGC BsaAI 16 CACGTG [CT]AC^GT[GA] BsaI 1042 GAGACC GGTCTC(1/5) BsiHKAI 1066 GAGCTC G[AT]GC[AT]^C BsmBI 938 GAGACG CGTCTC(1/5) BsoBI 763 CTCGAG C^[CT]CG[GA]G BstEII 1194 GGTTACC G^GT[ACGT]ACC BstPI 1194 GGTTACC G^GT[ACGT]ACC BstUI 112 CGCG CG^CG CelII 589 GCTCAGC GC^T[ACGT]AGC CfoI 1488 GCGC GCG^C DsaI 1199 CCATGG C^C[GA][CT]GG EaeI 258 TGGCCG [CT]^GGCC[GA] EcoO65I 1194 GGTTACC G^GT[ACGT]ACC EcoRI 1303 GAATTC G^AATTC HaeII 1487 GGCGCC [GA]GCGC^[CT] HgaI 628 GCGTC GACGC(5/10) HhaI 1488 GCGC GCG^C HinP1I 1488 GCGC G^CGC HincII 364 GTCAAC GT[CT]^[GA]AC HindII 364 GTCAAC GT[CT]^[GA]AC HindIII 1231 AAGCTT A^AGCTT KasI 1487 GGCGCC G^GCGCC MaeI 344 CTAG C^TAG MslI 612 CATTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 112 CGCG CG^CG NarI 1487 GGCGCC GG^CGCC NcoI 1199 CCATGG C^CATGG NspI 140 ACATGC [GA]CATG^[CT] PaeR7I 763 CTCGAG C^TCGAG PflMI 1332 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 16 CACGTG CAC^GTG PmlI 16 CACGTG CAC^GTG SacI 1066 GAGCTC GAGCT^C SbfI 1167 CCTGCAGG CCTGCA^GG SfoI 1487 GGCGCC GGC^GCC Sse8387I 1167 CCTGCAGG CCTGCA^GG SspI 250 AATATT AAT^ATT StuI 236 AGGCCT AGG^CCT Tsp45I 1297 GTGAC ^GT[GC]AC Tsp509I 1304 AATT ^AATT Van91I 1332 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 763 CTCGAG C^TCGAG XmnI 1050 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 627 GGCGTC G[GA]^CG[CT]C 1487 GGCGCC BbsI 1431 GAAGAC GAAGAC(2/6) 1241 GTCTTC BcgI 942 CGATGCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 41 GCAAGCTGCTCG BcnI 891 CCCGG CC^[GC]GG 1046 CCCGG BpuAI 1431 GAAGAC GAAGAC(2/6) 1241 GTCTTC BsaHI 627 GGCGTC G[GA]^CG[CT]C 1487 GGCGCC BsgI 688 GTGCAG GTGCAG(16/14) 1363 GTGCAG BsmAI 938 GAGAC GTCTC(1/5) 1042 GAGAC EarI 267 CTCTTC CTCTTC(1/4) 154 GAAGAG EcoNI 543 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1274 CCTCACCAAGG HapII 892 CCGG C^CGG 1047 CCGG Hin1I 627 GGCGTC G[GA]^CG[CT]C 1487 GGCGCC HpaII 892 CCGG C^CGG 1047 CCGG Ksp632I 267 CTCTTC CTCTTC(1/4) 154 GAAGAG MaeII 17 ACGT A^CGT 617 ACGT MaeIII 1195 GTTAC ^GT[ACGT]AC 1297 GTGAC MspI 892 CCGG C^CGG 1047 CCGG NciI 891 CCCGG CC^[GC]GG 1046 CCCGG PpuMI 178 GGGTCCT [GA]G^G[AT]CC[CT] 551 AGGACCT XcmI 165 CCAGCTGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1181 CCAAAGAGAAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MboII MflI MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI TspRI TthHB8I XhoII