(Circular) (Six-base) MAPSORT of: pFN21AE3223.seq Check: 5933 from: 1 to: 6223 pFN21AE3223 6223 bp With 182 enzymes: SgfI * March 28, 2012 12:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2659 2776 2659 Size: 117 6106 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3566 1937 Size: 1629 4594 Acc65I G'GTAC_C Cuts at: 3544 3544 Size: 6223 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 3233 3347 4286 5670 5938 1632 Size: 779 822 114 939 1384 268 1917 Fragments arranged by size: 1917 1384 939 822 779 268 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 2622 5122 6099 1203 Size: 1419 2500 977 1327 Fragments arranged by size: 2500 1419 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6223 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2977 3009 2977 Size: 32 6191 AlwNI CAG_nnn'CTG Cuts at: 2101 2176 2419 2465 2605 2837 3343 3457 3966 Size: 75 243 46 140 232 506 114 509 Cuts at: 3966 5538 2101 Size: 1572 2786 Fragments arranged by size: 2786 1572 509 506 243 232 140 114 75 46 ApaI G_GGCC'C Cuts at: 3017 3017 Size: 6223 ApaLI G'TGCA_C Cuts at: 4280 5436 4280 Size: 1156 5067 ApoI r'AATT_y Cuts at: 1493 3308 3532 3758 3910 6019 1493 Size: 1815 224 226 152 2109 1697 Fragments arranged by size: 2109 1815 1697 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 2768 3548 1870 Size: 73 825 780 4545 Fragments arranged by size: 4545 825 780 73 BamHI G'GATC_C Cuts at: 2914 3553 3920 2914 Size: 639 367 5217 Fragments arranged by size: 5217 639 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3492 3544 4999 618 Size: 325 217 627 1705 52 1455 1842 Fragments arranged by size: 1842 1705 1455 627 325 217 52 BanII G_rGCy'C Cuts at: 1391 2660 2745 3017 3075 3542 1391 Size: 1269 85 272 58 467 4072 Fragments arranged by size: 4072 1269 467 272 85 58 BbeI G_GCGC'C Cuts at: 3496 3496 Size: 6223 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3239 3443 928 Size: 971 1340 204 3708 Fragments arranged by size: 3708 1340 971 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2035 2069 2936 2970 4414 4448 1903 Size: 34 98 34 867 34 1444 34 3678 Fragments arranged by size: 3678 1444 867 98 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4115 5325 4115 Size: 1210 5013 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4834 136 Size: 107 122 71 631 605 3162 1525 Fragments arranged by size: 3162 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3087 6218 3087 Size: 3131 3092 BlpI GC'TnA_GC Cuts at: 2595 3640 2595 Size: 1045 5178 Bme1580I G_kGCm'C Cuts at: 1790 2028 3017 4284 5440 1790 Size: 238 989 1267 1156 2573 Fragments arranged by size: 2573 1267 1156 989 238 BmgBI CAC'GTC Cuts at: 1318 2623 1318 Size: 1305 4918 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2818 2893 2947 Size: 416 53 83 399 24 1401 75 54 Cuts at: 2947 3954 4912 442 Size: 1007 958 1753 Fragments arranged by size: 1753 1401 1007 958 416 399 83 75 54 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 6223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2940 2979 3147 3239 3299 3428 3452 4883 Size: 1701 39 168 92 60 129 24 1431 Cuts at: 4883 1239 Size: 2579 Fragments arranged by size: 2579 1701 1431 168 129 92 60 39 24 Bpu10I CC'TnA_GC Cuts at: 2781 3414 2781 Size: 633 5590 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3104 3410 4348 5213 5511 3104 Size: 306 938 865 298 3816 Fragments arranged by size: 3816 938 865 306 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3041 4886 882 Size: 617 1542 1845 2219 Fragments arranged by size: 2219 1845 1542 617 BsaAI yAC'GTr Cuts at: 493 1204 2023 4014 493 Size: 711 819 1991 2702 Fragments arranged by size: 2702 1991 819 711 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2633 3493 4030 4412 Size: 53 83 186 936 1100 860 537 382 Cuts at: 4412 5047 275 Size: 635 1451 Fragments arranged by size: 1451 1100 936 860 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4656 5054 5328 5475 5886 4656 Size: 398 274 147 411 4993 Fragments arranged by size: 4993 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2435 2465 2977 3007 1178 Size: 30 165 30 1032 30 512 30 4394 Fragments arranged by size: 4394 1032 512 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2459 2462 2547 2786 3137 3407 1186 Size: 1273 3 85 239 351 270 4002 Fragments arranged by size: 4002 1273 351 270 239 85 3 BseYI C'CCAG_C Cuts at: 2169 3336 5426 5922 2169 Size: 1167 2090 496 2470 Fragments arranged by size: 2470 2090 1167 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2714 3389 1163 Size: 573 978 675 3997 Fragments arranged by size: 3997 978 675 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3673 4434 5462 1540 Size: 275 186 1672 761 1028 2301 Fragments arranged by size: 2301 1672 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3075 4284 4369 5440 3075 Size: 1209 85 1071 3858 Fragments arranged by size: 3858 1209 1071 85 BsmI GAATG_Cn' Cuts at: 3734 3827 3734 Size: 93 6130 BsmBI CGTCTCn'nnnn_ Cuts at: 2937 2937 Size: 6223 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2660 2745 3017 3075 3542 4284 Size: 399 238 632 85 272 58 467 742 Cuts at: 4284 4369 5440 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 632 467 399 272 238 85 85 58 BspEI T'CCGG_A Cuts at: 5886 5886 Size: 6223 BspHI T'CATG_A Cuts at: 4112 5842 4112 Size: 1730 4493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2568 2659 2776 3432 3578 844 Size: 1724 91 117 656 146 3489 Fragments arranged by size: 3489 1724 656 146 117 91 BsrBI CCG'CTC Cuts at: 1394 1823 4110 1394 Size: 429 2287 3507 Fragments arranged by size: 3507 2287 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4718 4892 65 Size: 1183 3470 174 1396 Fragments arranged by size: 3470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4867 5054 1700 Size: 110 3057 187 2869 Fragments arranged by size: 3057 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 4283 5295 4283 Size: 1012 5211 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 2674 2798 794 Size: 1671 209 124 4219 Fragments arranged by size: 4219 1671 209 124 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 3199 1184 Size: 2015 4208 BstYI r'GATC_y Cuts at: 1972 2379 2914 3087 3223 3553 3920 4305 4322 Size: 407 535 173 136 330 367 385 17 Cuts at: 4322 5774 5860 5872 6218 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 535 407 385 367 346 330 173 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3204 513 Size: 552 939 1200 3532 Fragments arranged by size: 3532 1200 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5484 Fragments arranged by size: 5484 683 56 BtsI GCAGTG_nn' Cuts at: 2077 2203 2838 3029 3114 3739 4533 4553 2077 Size: 126 635 191 85 625 794 20 3747 Fragments arranged by size: 3747 794 635 625 191 126 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3273 3308 529 Size: 35 2709 35 3444 Fragments arranged by size: 3444 2709 35 35 DraI TTT'AAA Cuts at: 3527 3527 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5230 809 Size: 507 3914 1802 Fragments arranged by size: 3914 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2263 3670 4559 Size: 54 1425 50 275 18 433 1407 889 Cuts at: 4559 6188 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1407 889 433 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2278 4153 1353 Size: 801 124 1875 3423 Fragments arranged by size: 3423 1875 801 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4810 5184 5330 387 Size: 1064 138 3221 374 146 1280 Fragments arranged by size: 3221 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3073 3073 Size: 6223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2411 2940 2979 3147 3233 3239 3299 Size: 393 779 529 39 168 86 6 60 Cuts at: 3299 3347 3428 3452 4286 4883 5670 5938 1239 Size: 48 81 24 834 597 787 268 1524 Fragments arranged by size: 1524 834 787 779 597 529 393 268 168 86 81 60 48 39 24 6 EcoNI CCTnn'n_nnAGG Cuts at: 2552 3283 2552 Size: 731 5492 EcoO109I rG'GnC_Cy Cuts at: 2184 2557 3013 2184 Size: 373 456 5394 Fragments arranged by size: 5394 456 373 EcoRI G'AATT_C Cuts at: 3308 3532 3910 3308 Size: 224 378 5621 Fragments arranged by size: 5621 378 224 FspI TGC'GCA Cuts at: 4729 4729 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 3496 5370 1678 Size: 1818 1874 2531 Fragments arranged by size: 2531 1874 1818 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 2751 2783 2819 Size: 32 135 32 721 32 426 32 36 Cuts at: 2819 2851 3040 3072 3122 3154 4864 4896 4938 Size: 32 189 32 50 32 1710 32 42 Cuts at: 4938 4970 1373 Size: 32 2626 Fragments arranged by size: 2626 1710 721 426 189 135 50 42 36 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2371 3567 3813 669 Size: 988 281 433 1196 246 3079 Fragments arranged by size: 3079 1196 988 433 281 246 HindIII A'AGCT_T Cuts at: 748 3236 748 Size: 2488 3735 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KasI G'GCGC_C Cuts at: 3492 3492 Size: 6223 KpnI G_GTAC'C Cuts at: 3548 3548 Size: 6223 MfeI C'AATT_G Cuts at: 3822 3822 Size: 6223 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5337 5521 1305 Size: 4032 184 2007 Fragments arranged by size: 4032 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4401 Fragments arranged by size: 4401 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 4181 4540 4699 518 Size: 648 1455 1560 359 159 2042 Fragments arranged by size: 2042 1560 1455 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2173 2281 2419 2572 2591 3157 3253 3340 Size: 567 108 138 153 19 566 96 87 Cuts at: 3340 3489 3639 4316 5464 5709 1606 Size: 149 150 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 567 566 245 153 150 149 138 108 96 87 19 NarI GG'CG_CC Cuts at: 3493 3493 Size: 6223 NcoI C'CATG_G Cuts at: 513 1065 2004 3204 513 Size: 552 939 1200 3532 Fragments arranged by size: 3532 1200 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6223 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6223 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 2149 3581 5126 1132 Size: 97 920 1432 1545 2229 Fragments arranged by size: 2229 1545 1432 920 97 PasI CC'CwG_GG Cuts at: 1348 2605 1348 Size: 1257 4966 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 3343 1108 Size: 2235 3988 PfoI T'CCnGG_A Cuts at: 2139 2217 2895 2917 2139 Size: 78 678 22 5445 Fragments arranged by size: 5445 678 78 22 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PmlI CAC'GTG Cuts at: 2023 2023 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2977 3009 4292 4324 5805 5837 2977 Size: 32 1283 32 1481 32 3363 Fragments arranged by size: 3363 1481 1283 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2184 2557 2184 Size: 373 5850 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PspOMI G'GGCC_C Cuts at: 3013 3013 Size: 6223 PspXI vC'TCGA_Gb Cuts at: 2768 2768 Size: 6223 PstI C_TGCA'G Cuts at: 830 2084 2424 3177 3453 3575 830 Size: 1254 340 753 276 122 3478 Fragments arranged by size: 3478 1254 753 340 276 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 PvuII CAG'CTG Cuts at: 2173 2419 2572 3157 3253 3340 2173 Size: 246 153 585 96 87 5056 Fragments arranged by size: 5056 585 246 153 96 87 SacI G_AGCT'C Cuts at: 3075 3075 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SbfI CC_TGCA'GG Cuts at: 3177 3575 3177 Size: 398 5825 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SfcI C'TryA_G Cuts at: 826 1045 2080 2420 3173 3449 3571 4706 5387 Size: 219 1035 340 753 276 122 1135 681 Cuts at: 5387 5578 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1035 753 681 340 276 219 191 122 SfoI GGC'GCC Cuts at: 3494 3494 Size: 6223 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 3550 1872 Size: 1678 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2768 3083 3389 4327 5228 5490 Size: 197 926 825 315 306 938 901 262 Cuts at: 5490 820 Size: 1553 Fragments arranged by size: 1553 938 926 901 825 315 306 262 197 SnaBI TAC'GTA Cuts at: 493 4014 493 Size: 3521 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 2257 4147 5 Size: 47 2205 1890 2081 Fragments arranged by size: 2205 2081 1890 47 StuI AGG'CCT Cuts at: 1517 2243 1517 Size: 726 5497 StyI C'CwwG_G Cuts at: 513 1065 2004 3204 3284 3441 3662 513 Size: 552 939 1200 80 157 221 3074 Fragments arranged by size: 3074 1200 939 552 221 157 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3312 4250 4267 4420 4605 5050 325 Size: 1255 1732 938 17 153 185 445 1498 Fragments arranged by size: 1732 1498 1255 938 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4469 6181 96 Size: 274 80 33 51 1443 2492 1712 138 Fragments arranged by size: 2492 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2435 2467 6183 6215 2435 Size: 32 3716 32 2443 Fragments arranged by size: 3716 2443 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6223 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2177 3193 2177 Size: 1016 5207 XhoI C'TCGA_G Cuts at: 1943 2768 1943 Size: 825 5398 XmaI C'CCGG_G Cuts at: 1870 3548 1870 Size: 1678 4545 XmnI GAAnn'nnTTC Cuts at: 3059 4352 3059 Size: 1293 4930 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI