(Linear) (Six-base) MAPSORT of: KIEE3223.seq Check: 3980 from: 1 to: 1518 KIEE3223.orf With 182 enzymes: SgfI * March 22, 2012 16:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 654 771 1518 Size: 654 117 747 Fragments arranged by size: 747 654 117 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 1228 1342 1518 Size: 406 822 114 176 Fragments arranged by size: 822 406 176 114 AflIII A'CryG_T Cuts at: 0 617 1518 Size: 617 901 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 972 1004 1518 Size: 972 32 514 Fragments arranged by size: 972 514 32 AlwNI CAG_nnn'CTG Cuts at: 0 96 171 414 460 600 832 1338 1452 Size: 96 75 243 46 140 232 506 114 Cuts at: 1452 1518 Size: 66 Fragments arranged by size: 506 243 232 140 114 96 75 66 46 ApaI G_GGCC'C Cuts at: 0 1012 1518 Size: 1012 506 ApoI r'AATT_y Cuts at: 0 1303 1518 Size: 1303 215 AvaI C'yCGr_G Cuts at: 0 763 1518 Size: 763 755 BamHI G'GATC_C Cuts at: 0 909 1518 Size: 909 609 BanI G'GyrC_C Cuts at: 0 1487 1518 Size: 1487 31 BanII G_rGCy'C Cuts at: 0 655 740 1012 1070 1518 Size: 655 85 272 58 448 Fragments arranged by size: 655 448 272 85 58 BbeI G_GCGC'C Cuts at: 0 1491 1518 Size: 1491 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 1234 1438 1518 Size: 1234 204 80 Fragments arranged by size: 1234 204 80 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 30 64 931 965 1518 Size: 30 34 867 34 553 Fragments arranged by size: 867 553 34 34 30 BglII A'GATC_T Cuts at: 0 1082 1518 Size: 1082 436 BlpI GC'TnA_GC Cuts at: 0 590 1518 Size: 590 928 Bme1580I G_kGCm'C Cuts at: 0 23 1012 1518 Size: 23 989 506 Fragments arranged by size: 989 506 23 BmgBI CAC'GTC Cuts at: 0 618 1518 Size: 618 900 BmrI ACTGGGnnnn_n' Cuts at: 0 813 888 942 1518 Size: 813 75 54 576 Fragments arranged by size: 813 576 75 54 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 396 1518 Size: 396 1122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 935 974 1142 1234 1294 1423 1447 1518 Size: 935 39 168 92 60 129 24 71 Fragments arranged by size: 935 168 129 92 71 60 39 24 Bpu10I CC'TnA_GC Cuts at: 0 776 1409 1518 Size: 776 633 109 Fragments arranged by size: 776 633 109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1099 1405 1518 Size: 1099 306 113 Fragments arranged by size: 1099 306 113 BsaI GGTCTCn'nnnn_ Cuts at: 0 1036 1518 Size: 1036 482 BsaAI yAC'GTr Cuts at: 0 18 1518 Size: 18 1500 BsaHI Gr'CG_yC Cuts at: 0 628 1488 1518 Size: 628 860 30 Fragments arranged by size: 860 628 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 430 460 972 1002 1518 Size: 430 30 512 30 516 Fragments arranged by size: 516 512 430 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 454 457 542 781 1132 1402 1518 Size: 454 3 85 239 351 270 116 Fragments arranged by size: 454 351 270 239 116 85 3 BseYI C'CCAG_C Cuts at: 0 164 1331 1518 Size: 164 1167 187 Fragments arranged by size: 1167 187 164 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 1384 1518 Size: 709 675 134 Fragments arranged by size: 709 675 134 BsiHKAI G_wGCw'C Cuts at: 0 1070 1518 Size: 1070 448 BsmBI CGTCTCn'nnnn_ Cuts at: 0 932 1518 Size: 932 586 Bsp1286I G_dGCh'C Cuts at: 0 23 655 740 1012 1070 1518 Size: 23 632 85 272 58 448 Fragments arranged by size: 632 448 272 85 58 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 563 654 771 1427 1518 Size: 563 91 117 656 91 Fragments arranged by size: 656 563 117 91 91 BstAPI GCAn_nnn'nTGC Cuts at: 0 460 669 793 1518 Size: 460 209 124 725 Fragments arranged by size: 725 460 209 124 BstEII G'GTnAC_C Cuts at: 0 1194 1518 Size: 1194 324 BstYI r'GATC_y Cuts at: 0 374 909 1082 1218 1518 Size: 374 535 173 136 300 Fragments arranged by size: 535 374 300 173 136 BtgI C'CryG_G Cuts at: 0 1199 1518 Size: 1199 319 BtsI GCAGTG_nn' Cuts at: 0 72 198 833 1024 1109 1518 Size: 72 126 635 191 85 409 Fragments arranged by size: 635 409 191 126 85 72 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1268 1303 1518 Size: 1268 35 215 Fragments arranged by size: 1268 215 35 EaeI y'GGCC_r Cuts at: 0 258 1518 Size: 258 1260 EarI CTCTTCn'nnn_ Cuts at: 0 149 273 1518 Size: 149 124 1245 Fragments arranged by size: 1245 149 124 EcoICRI GAG'CTC Cuts at: 0 1068 1518 Size: 1068 450 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 935 974 1142 1228 1234 1294 1342 Size: 406 529 39 168 86 6 60 48 Cuts at: 1342 1423 1447 1518 Size: 81 24 71 Fragments arranged by size: 529 406 168 86 81 71 60 48 39 24 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 547 1278 1518 Size: 547 731 240 Fragments arranged by size: 731 547 240 EcoO109I rG'GnC_Cy Cuts at: 0 179 552 1008 1518 Size: 179 373 456 510 Fragments arranged by size: 510 456 373 179 EcoRI G'AATT_C Cuts at: 0 1303 1518 Size: 1303 215 HaeII r_GCGC'y Cuts at: 0 1491 1518 Size: 1491 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 288 320 746 778 814 846 1035 1067 Size: 288 32 426 32 36 32 189 32 Cuts at: 1067 1117 1149 1518 Size: 50 32 369 Fragments arranged by size: 426 369 288 189 50 36 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 366 1518 Size: 366 1152 HindIII A'AGCT_T Cuts at: 0 1231 1518 Size: 1231 287 KasI G'GCGC_C Cuts at: 0 1487 1518 Size: 1487 31 MslI CAynn'nnrTG Cuts at: 0 616 1518 Size: 616 902 MspA1I CmG'CkG Cuts at: 0 168 276 414 567 586 1152 1248 1335 Size: 168 108 138 153 19 566 96 87 Cuts at: 1335 1484 1518 Size: 149 34 Fragments arranged by size: 566 168 153 149 138 108 96 87 34 19 NarI GG'CG_CC Cuts at: 0 1488 1518 Size: 1488 30 NcoI C'CATG_G Cuts at: 0 1199 1518 Size: 1199 319 NspI r_CATG'y Cuts at: 0 144 1518 Size: 144 1374 PasI CC'CwG_GG Cuts at: 0 600 1518 Size: 600 918 PflMI CCAn_nnn'nTGG Cuts at: 0 1338 1518 Size: 1338 180 PfoI T'CCnGG_A Cuts at: 0 134 212 890 912 1518 Size: 134 78 678 22 606 Fragments arranged by size: 678 606 134 78 22 PmlI CAC'GTG Cuts at: 0 18 1518 Size: 18 1500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 972 1004 1518 Size: 972 32 514 Fragments arranged by size: 972 514 32 PpuMI rG'GwC_Cy Cuts at: 0 179 552 1518 Size: 179 373 966 Fragments arranged by size: 966 373 179 PspOMI G'GGCC_C Cuts at: 0 1008 1518 Size: 1008 510 PspXI vC'TCGA_Gb Cuts at: 0 763 1518 Size: 763 755 PstI C_TGCA'G Cuts at: 0 79 419 1172 1448 1518 Size: 79 340 753 276 70 Fragments arranged by size: 753 340 276 79 70 PvuII CAG'CTG Cuts at: 0 168 414 567 1152 1248 1335 1518 Size: 168 246 153 585 96 87 183 Fragments arranged by size: 585 246 183 168 153 96 87 SacI G_AGCT'C Cuts at: 0 1070 1518 Size: 1070 448 SbfI CC_TGCA'GG Cuts at: 0 1172 1518 Size: 1172 346 SfcI C'TryA_G Cuts at: 0 75 415 1168 1444 1518 Size: 75 340 753 276 74 Fragments arranged by size: 753 340 276 75 74 SfoI GGC'GCC Cuts at: 0 1489 1518 Size: 1489 29 SmlI C'TyrA_G Cuts at: 0 763 1078 1384 1518 Size: 763 315 306 134 Fragments arranged by size: 763 315 306 134 SspI AAT'ATT Cuts at: 0 252 1518 Size: 252 1266 StuI AGG'CCT Cuts at: 0 238 1518 Size: 238 1280 StyI C'CwwG_G Cuts at: 0 1199 1279 1436 1518 Size: 1199 80 157 82 Fragments arranged by size: 1199 157 82 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1307 1518 Size: 1307 211 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 430 462 1518 Size: 430 32 1056 Fragments arranged by size: 1056 430 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 172 1188 1518 Size: 172 1016 330 Fragments arranged by size: 1016 330 172 XhoI C'TCGA_G Cuts at: 0 763 1518 Size: 763 755 XmnI GAAnn'nnTTC Cuts at: 0 1054 1518 Size: 1054 464 Enzymes that do cut: AarI AcuI AflIII AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BstAPI BstEII BstYI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI MslI MspA1I NarI NcoI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SbfI SfcI SfoI SmlI SspI StuI StyI TaqII TstI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XmaI ZraI