################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AhdI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsiEI BsiWI BsmI BspDI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 286 CGCG CG^CG AccIII 1121 TCCGGA T^CCGGA AgeI 1136 ACCGGT A^CCGGT Alw44I 340 GTGCAC G^TGCAC ApaI 819 GGGCCC GGGCC^C ApaLI 340 GTGCAC G^TGCAC BbsI 1027 GTCTTC GAAGAC(2/6) BclI 842 TGATCA T^GATCA BfaI 187 CTAG C^TAG BglI 1165 GCCCGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 73 CTCCAG CTGGAG(16/14) BpuAI 1027 GTCTTC GAAGAC(2/6) BsaBI 687 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1121 TCCGGA T^CCGGA BseRI 322 GAGGAG GAGGAG(10/8) BsmFI 99 GTCCC GGGAC(10/14) Bsp1407I 797 TGTACA T^GTACA BspEI 1121 TCCGGA T^CCGGA BspHI 881 TCATGA T^CATGA BsrGI 797 TGTACA T^GTACA BsrI 464 ACTGG ACTGG(1/-1) BstAPI 437 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 286 CGCG CG^CG BstYI 311 AGATCC [GA]^GATC[CT] DsaI 140 CCGTGG C^C[GA][CT]GG EarI 1125 GAAGAG CTCTTC(1/4) EcoNI 315 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 842 TGATCA T^GATCA HinfI 1054 GAATC G^A[ACGT]TC Ksp632I 1125 GAAGAG CTCTTC(1/4) MaeI 187 CTAG C^TAG MamI 687 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 311 AGATCC [GA]^GATC[CT] MroI 1121 TCCGGA T^CCGGA MspA1I 273 CAGCTG C[AC]G^C[GT]G MvnI 286 CGCG CG^CG NspI 980 ACATGC [GA]CATG^[CT] PinAI 1136 ACCGGT A^CCGGT PstI 124 CTGCAG CTGCA^G PvuII 273 CAGCTG CAG^CTG RcaI 881 TCATGA T^CATGA SexAI 502 ACCTGGT A^CC[AT]GGT SfcI 124 CTGCAG C^T[GA][CT]AG SgrAI 1135 CACCGGTG C[GA]^CCGG[CT]G SspBI 797 TGTACA T^GTACA StuI 787 AGGCCT AGG^CCT TaqI 1156 TCGA T^CGA TfiI 1054 GAATC G^A[AT]TC TthHB8I 1156 TCGA T^CGA XcmI 500 CCACCTGGTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 311 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspHI 340 GTGCAC G[AT]GC[AT]^C 652 GTGCTC AviII 491 TGCGCA TGC^GCA 677 TGCGCA BalI 104 TGGCCA TGG^CCA 263 TGGCCA BanII 528 GAGCCC G[GA]GC[CT]^C 819 GGGCCC BsaI 913 GAGACC GGTCTC(1/5) 1014 GGTCTC BsgI 637 CTGCAC GTGCAG(16/14) 561 GTGCAG BsiHKAI 340 GTGCAC G[AT]GC[AT]^C 652 GTGCTC BsmBI 22 CGTCTC CGTCTC(1/5) 60 CGTCTC BsrFI 671 ACCGGC [GA]^CCGG[CT] 1136 ACCGGT BstXI 722 CCATCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 824 CCATGATCCTGG CfoI 492 GCGC GCG^C 678 GCGC Cfr10I 671 ACCGGC [GA]^CCGG[CT] 1136 ACCGGT EaeI 104 TGGCCA [CT]^GGCC[GA] 263 TGGCCA FspI 491 TGCGCA TGC^GCA 677 TGCGCA HhaI 492 GCGC GCG^C 678 GCGC HinP1I 492 GCGC G^CGC 678 GCGC MaeII 551 ACGT A^CGT 907 ACGT MaeIII 571 GTGAC ^GT[ACGT]AC 598 GTGAC MluNI 104 TGGCCA TGG^CCA 263 TGGCCA MscI 104 TGGCCA TGG^CCA 263 TGGCCA SmaI 1000 CCCGGG CCC^GGG 1019 CCCGGG Tsp45I 571 GTGAC ^GT[GC]AC 598 GTGAC Tsp509I 292 AATT ^AATT 847 AATT XmaCI 1000 CCCGGG C^CCGGG 1019 CCCGGG XmaI 1000 CCCGGG C^CCGGG 1019 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BanI BbvI BcnI BmyI BsaJI BsaWI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI