################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII BalI BbeI BbrPI BbsI BbvCI BciVI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KspI MaeII MamI MluI MluNI MroI MscI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 895 GGTACC G^GTACC AccII 72 CGCG CG^CG AcyI 1014 GACGCC G[GA]^CG[CT]C Alw44I 657 GTGCAC G^TGCAC AlwNI 796 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 727 GGGCCC GGGCC^C ApaLI 657 GTGCAC G^TGCAC Asp718I 895 GGTACC G^GTACC AspI 76 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 724 CTCGGG C^[CT]CG[GA]G AvrII 969 CCTAGG C^CTAGG BamHI 540 GGATCC G^GATCC BcgI 335 GCAGCCGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 803 TGATCA T^GATCA BlnI 969 CCTAGG C^CTAGG BpmI 304 CTGGAG CTGGAG(16/14) BsaHI 1014 GACGCC G[GA]^CG[CT]C BsaI 580 GAGACC GGTCTC(1/5) BsiWI 708 CGTACG C^GTACG BsmAI 580 GAGAC GTCTC(1/5) BsoBI 724 CTCGGG C^[CT]CG[GA]G BstF5I 883 GGATG GGATG(2/0) BstUI 72 CGCG CG^CG CfoI 672 GCGC GCG^C EcoT14I 969 CCTAGG C^C[AT][AT]GG FauI 287 CCCGC CCCGC(4/6) FbaI 803 TGATCA T^GATCA FokI 883 GGATG GGATG(9/13) HgaI 1014 GACGC GACGC(5/10) HhaI 672 GCGC GCG^C Hin1I 1014 GACGCC G[GA]^CG[CT]C HinP1I 672 GCGC G^CGC KpnI 895 GGTACC GGTAC^C MfeI 261 CAATTG C^AATTG MunI 261 CAATTG C^AATTG MvnI 72 CGCG CG^CG NaeI 809 GCCGGC GCC^GGC NgoMIV 809 GCCGGC G^CCGGC PflFI 76 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 910 GAGTC GAGTC(4/5) PpuMI 664 AGGTCCC [GA]G^G[AT]CC[CT] SacI 756 GAGCTC GAGCT^C SapI 237 GAAGAGC GCTCTTC(1/4) SexAI 77 ACCTGGT A^CC[AT]GGT SfaNI 1022 GCATC GCATC(5/9) StyI 969 CCTAGG C^C[AT][AT]GG Tth111I 76 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 657 GTGCAC G[AT]GC[AT]^C 756 GAGCTC BanI 895 GGTACC G^G[CT][GA]CC 1005 GGCACC BanII 727 GGGCCC G[GA]GC[CT]^C 756 GAGCTC BcnI 120 CCGGG CC^[GC]GG 199 CCGGG BsiHKAI 657 GTGCAC G[AT]GC[AT]^C 756 GAGCTC BsrDI 276 CATTGC GCAATG(2/0) 621 GCAATG BsrFI 809 GCCGGC [GA]^CCGG[CT] 1002 ACCGGC BstYI 496 AGATCC [GA]^GATC[CT] 540 GGATCC Cac8I 608 GCCTGC GC[ACGT]^[ACGT]GC 809 GCCGGC Cfr10I 809 GCCGGC [GA]^CCGG[CT] 1002 ACCGGC EarI 237 GAAGAG CTCTTC(1/4) 459 GAAGAG EcoNI 78 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 741 CCTTCCAGAGG HphI 630 GGTGA GGTGA(8/7) 841 TCACC Ksp632I 237 GAAGAG CTCTTC(1/4) 459 GAAGAG MaeIII 392 GTGAC ^GT[ACGT]AC 912 GTCAC MflI 496 AGATCC [GA]^GATC[CT] 540 GGATCC MseI 508 TTAA T^TAA 548 TTAA MslI 514 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 685 CACACAGATG NciI 120 CCGGG CC^[GC]GG 199 CCGGG NlaIII 171 CATG CATG^ 783 CATG Tru9I 508 TTAA T^TAA 548 TTAA Tsp45I 392 GTGAC ^GT[GC]AC 912 GTCAC TspRI 232 CAGTG CA[GC]TG[ACGT][ACGT]^ 817 CAGTG XhoII 496 AGATCC [GA]^GATC[CT] 540 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BfaI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TfiI TseI Tsp509I TthHB8I