Restriction map for
pFN21AE2242
(Circular) (Six-base) MAPSORT of: pFN21AE2242.seq Check: 2222 from: 1 to: 5746
pFN21AE2242 5746 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 2900 3067 2900
Size: 167 5579
AclI AA'CG_TT
Cuts at: 1563 3045 3873 4246 1563
Size: 1482 828 373 3063
Fragments arranged by size:
3063 1482 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 3809 5193 5461 1632
Size: 1172 1005 1384 268 1917
Fragments arranged by size:
1917 1384 1172 1005 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 2474
Size: 32 5714
AlwNI CAG_nnn'CTG
Cuts at: 2806 3489 5061 2806
Size: 683 1572 3491
Fragments arranged by size:
3491 1572 683
ApaI G_GGCC'C
Cuts at: 2736 2736
Size: 5746
ApaLI G'TGCA_C
Cuts at: 2662 3803 4959 2662
Size: 1141 1156 3449
Fragments arranged by size:
3449 1156 1141
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2729 3071 1870
Size: 73 786 342 4545
Fragments arranged by size:
4545 786 342 73
AvrII C'CTAG_G
Cuts at: 2974 2974
Size: 5746
BamHI G'GATC_C
Cuts at: 2545 3076 3443 2545
Size: 531 367 4848
Fragments arranged by size:
4848 531 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2900 3010 3067 4522 618
Size: 325 217 627 1113 110 57 1455 1842
Fragments arranged by size:
1842 1455 1113 627 325 217 110 57
BanII G_rGCy'C
Cuts at: 1391 2736 2765 3065 1391
Size: 1345 29 300 4072
Fragments arranged by size:
4072 1345 300 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2329 2363 3937 3971 1903
Size: 34 392 34 1574 34 3678
Fragments arranged by size:
3678 1574 392 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 2808 1548
Size: 1260 4486
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4357 136
Size: 107 122 71 631 605 2685 1525
Fragments arranged by size:
2685 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2666 2736 3807 4963 1790
Size: 876 70 1071 1156 2573
Fragments arranged by size:
2573 1156 1071 876 70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2580 3477 4435
Size: 416 53 83 399 24 1163 897 958
Cuts at: 4435 442
Size: 1753
Fragments arranged by size:
1753 1163 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 4406 1239
Size: 1091 2076 2579
Fragments arranged by size:
2579 2076 1091
Bpu10I CC'TnA_GC
Cuts at: 2064 2064
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2579 4409 882
Size: 617 1080 1830 2219
Fragments arranged by size:
2219 1830 1080 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3020 3553 3935 4570
Size: 53 83 186 936 1487 533 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1487 1451 936 635 533 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 1178
Size: 30 165 30 603 30 4888
Fragments arranged by size:
4888 603 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 2824 2854 1186
Size: 1429 209 30 4078
Fragments arranged by size:
4078 1429 209 30
BseYI C'CCAG_C
Cuts at: 3023 4949 5445 3023
Size: 1926 496 3324
Fragments arranged by size:
3324 1926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5173
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2666 2765 3807 3892 4963 2666
Size: 99 1042 85 1071 3449
Fragments arranged by size:
3449 1071 1042 99 85
BsiWI C'GTAC_G
Cuts at: 2713 2713
Size: 5746
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2666 2736 2765 3065 3807 3892 4963
Size: 399 876 70 29 300 742 85 1071
Cuts at: 4963 1391
Size: 2174
Fragments arranged by size:
2174 1071 876 742 399 300 85 70
29
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 844
Size: 2257 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2280 2633 4241 4415 65
Size: 1183 1032 353 1608 174 1396
Fragments arranged by size:
1608 1396 1183 1032 353 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2814 3007 4390 4577 1700
Size: 110 1004 193 1383 187 2869
Fragments arranged by size:
2869 1383 1004 193 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2501 2545 3076 3443 3828 3845 5297 5383
Size: 529 44 531 367 385 17 1452 86
Cuts at: 5383 5395 5741 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 531 529 385 367 346 86
44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 487
Size: 683 56 818 4189
Fragments arranged by size:
4189 818 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3193 4082 5711
Size: 54 1425 50 275 18 1363 889 1629
Cuts at: 5711 8
Size: 43
Fragments arranged by size:
1629 1425 1363 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2459 3676 1353
Size: 884 222 1217 3423
Fragments arranged by size:
3423 1217 884 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2763 2763
Size: 5746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2804 3809 4406 5193 5461 1239
Size: 393 698 474 1005 597 787 268 1524
Fragments arranged by size:
1524 1005 787 698 597 474 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2750 2087
Size: 663 5083
EcoO109I rG'GnC_Cy
Cuts at: 2670 2733 2803 2670
Size: 63 70 5613
Fragments arranged by size:
5613 70 63
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2819 2846 2851
Size: 32 135 32 434 32 781 27 5
Cuts at: 2851 2866 2878 2898 4387 4419 4461 4493 1373
Size: 15 12 20 1489 32 42 32 2626
Fragments arranged by size:
2626 1489 781 434 135 42 32 32
32 32 32 27 20 15 12 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 2904 3071 2904
Size: 167 5579
MfeI C'AATT_G
Cuts at: 2266 3345 2266
Size: 1079 4667
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2312 3006 4860 5044 1305
Size: 1007 694 1854 184 2007
Fragments arranged by size:
2007 1854 1007 694 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3924
Fragments arranged by size:
3924 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2523 2694 3704 4063 4222 518
Size: 648 843 514 171 1010 359 159 2042
Fragments arranged by size:
2042 1010 843 648 514 359 171 159
MspA1I CmG'CkG
Cuts at: 1606 3162 3839 4987 5232 1606
Size: 1556 677 1148 245 2120
Fragments arranged by size:
2120 1556 1148 677 245
NaeI GCC'GGC
Cuts at: 2816 2816
Size: 5746
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NgoMIV G'CCGG_C
Cuts at: 2814 2814
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2590 1348
Size: 1242 4504
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2670 2670
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspOMI G'GGCC_C
Cuts at: 2732 2732
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
SacI G_AGCT'C
Cuts at: 2765 2765
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2237
Size: 5746
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SexAI A'CCwGG_T
Cuts at: 2082 2082
Size: 5746
SfcI C'TryA_G
Cuts at: 826 1045 3094 4229 4910 5101 826
Size: 219 2049 1135 681 191 1471
Fragments arranged by size:
2049 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3850 4751 5013 820
Size: 197 926 1907 901 262 1553
Fragments arranged by size:
1907 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2974 3185 513
Size: 552 1909 211 3074
Fragments arranged by size:
3074 1909 552 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3773 3790 3943 4128 4573 325
Size: 1255 2193 17 153 185 445 1498
Fragments arranged by size:
2193 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3992 5704 96
Size: 274 80 33 51 1443 2015 1712 138
Fragments arranged by size:
2015 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 2084 1316
Size: 768 4978
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BfrBI BplI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI