################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeII MamI MboI MboII MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 379 GGTACC G^GTACC AfaI 380 GTAC GT^AC AgeI 184 ACCGGT A^CCGGT Asp718I 379 GGTACC G^GTACC AspHI 336 GAGCAC G[AT]GC[AT]^C AvaII 53 GGACC G^G[AT]CC BalI 309 TGGCCA TGG^CCA BanII 520 GAGCCC G[GA]GC[CT]^C BglI 311 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 63 CTCCAG CTGGAG(16/14) BsaJI 446 CCGAGG C^C[ACGT][ACGT]GG BsaWI 184 ACCGGT [AT]^CCGG[AT] BsiHKAI 336 GAGCAC G[AT]GC[AT]^C BsrFI 184 ACCGGT [GA]^CCGG[CT] BssKI 312 CCAGG ^CC[ACGT]GG BstNI 312 CCAGG CC^[AT]GG Cfr10I 184 ACCGGT [GA]^CCGG[CT] Cfr13I 53 GGACC G^G[ACGT]CC DdeI 518 CTGAG C^T[ACGT]AG DraII 52 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 309 TGGCCA [CT]^GGCC[GA] EcoO109I 52 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 312 CCAGG ^CC[AT]GG HapII 185 CCGG C^CGG HinfI 199 GACTC G^A[ACGT]TC HpaII 185 CCGG C^CGG HphI 405 GGTGA GGTGA(8/7) KpnI 379 GGTACC GGTAC^C MaeIII 197 GTGAC ^GT[ACGT]AC MluNI 309 TGGCCA TGG^CCA MscI 309 TGGCCA TGG^CCA MspI 185 CCGG C^CGG MvaI 312 CCAGG CC^[AT]GG PinAI 184 ACCGGT A^CCGGT PleI 199 GACTC GAGTC(4/5) PpuMI 52 GGGACCT [GA]G^G[AT]CC[CT] RsaI 380 GTAC GT^AC Sau96I 53 GGACC G^G[ACGT]CC ScrFI 312 CCAGG CC^[ACGT]GG StuI 449 AGGCCT AGG^CCT Tsp45I 197 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BanI 353 GGTGCC G^G[CT][GA]CC 379 GGTACC BmyI 336 GAGCAC G[AGT]GC[ACT]^C 520 GAGCCC BsaI 91 GAGACC GGTCTC(1/5) 121 GAGACC BsgI 411 CTGCAC GTGCAG(16/14) 436 CTGCAC BsmAI 91 GAGAC GTCTC(1/5) 121 GAGAC BsmFI 52 GGGAC GGGAC(10/14) 190 GGGAC Bsp1286I 336 GAGCAC G[AGT]GC[ACT]^C 520 GAGCCC BspMI 55 ACCTGC ACCTGC(4/8) 382 ACCTGC BstF5I 422 CATCC GGATG(2/0) 467 CATCC FokI 422 CATCC GGATG(9/13) 467 CATCC TspRI 48 CAGTG CA[GC]TG[ACGT][ACGT]^ 324 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI AluI AlwNI BbvI BfaI BsiYI BslI BsrI Cac8I Fnu4HI HaeIII ItaI MaeI MnlI MspA1I MwoI NlaIV PvuII TseI