(Linear) (Six-base) MAPSORT of: KIEE6588.seq Check: 2754 from: 1 to: 531 KIEE6588.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. Acc65I G'GTAC_C Cuts at: 0 379 531 Size: 379 152 AgeI A'CCGG_T Cuts at: 0 184 531 Size: 184 347 AlwNI CAG_nnn'CTG Cuts at: 0 23 71 101 131 161 269 531 Size: 23 48 30 30 30 108 262 Fragments arranged by size: 262 108 48 30 30 30 23 BanI G'GyrC_C Cuts at: 0 353 379 531 Size: 353 26 152 Fragments arranged by size: 353 152 26 BanII G_rGCy'C Cuts at: 0 524 531 Size: 524 7 BglI GCCn_nnn'nGGC Cuts at: 0 317 531 Size: 317 214 BmrI ACTGGGnnnn_n' Cuts at: 0 490 531 Size: 490 41 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 114 531 Size: 114 417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 531 Size: 48 483 BsaI GGTCTCn'nnnn_ Cuts at: 0 85 115 531 Size: 85 30 416 Fragments arranged by size: 416 85 30 BsaWI w'CCGG_w Cuts at: 0 184 531 Size: 184 347 BseYI C'CCAG_C Cuts at: 0 34 531 Size: 34 497 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 396 421 531 Size: 396 25 110 Fragments arranged by size: 396 110 25 BsiHKAI G_wGCw'C Cuts at: 0 340 531 Size: 340 191 Bsp1286I G_dGCh'C Cuts at: 0 340 524 531 Size: 340 184 7 Fragments arranged by size: 340 184 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 64 391 531 Size: 64 327 140 Fragments arranged by size: 327 140 64 BsrFI r'CCGG_y Cuts at: 0 184 531 Size: 184 347 BtsI GCAGTG_nn' Cuts at: 0 330 531 Size: 330 201 EaeI y'GGCC_r Cuts at: 0 309 531 Size: 309 222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 531 Size: 48 483 EcoO109I rG'GnC_Cy Cuts at: 0 53 531 Size: 53 478 KpnI G_GTAC'C Cuts at: 0 383 531 Size: 383 148 MscI TGG'CCA Cuts at: 0 311 531 Size: 311 220 MspA1I CmG'CkG Cuts at: 0 38 68 128 435 531 Size: 38 30 60 307 96 Fragments arranged by size: 307 96 60 38 30 PpuMI rG'GwC_Cy Cuts at: 0 53 531 Size: 53 478 PvuII CAG'CTG Cuts at: 0 38 68 128 435 531 Size: 38 30 60 307 96 Fragments arranged by size: 307 96 60 38 30 StuI AGG'CCT Cuts at: 0 451 531 Size: 451 80 Enzymes that do cut: Acc65I AgeI AlwNI BanI BanII BglI BmrI BplI BpmI BsaI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BtsI EaeI Eco57MI EcoO109I KpnI MscI MspA1I PpuMI PvuII StuI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI