################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 575 GGTACC G^GTACC AccII 175 CGCG CG^CG Asp718I 575 GGTACC G^GTACC AspHI 267 GAGCAC G[AT]GC[AT]^C BanI 575 GGTACC G^G[CT][GA]CC BsiHKAI 267 GAGCAC G[AT]GC[AT]^C BspHI 389 TCATGA T^CATGA BsrI 251 ACTGG ACTGG(1/-1) BssSI 520 CTCGTG CACGAG(-5/-1) BstAPI 234 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 175 CGCG CG^CG CfoI 166 GCGC GCG^C DdeI 62 CTAAG C^T[ACGT]AG HaeII 165 GGCGCT [GA]GCGC^[CT] HhaI 166 GCGC GCG^C HinP1I 166 GCGC G^CGC HphI 192 GGTGA GGTGA(8/7) KpnI 575 GGTACC GGTAC^C MaeII 219 ACGT A^CGT MvnI 175 CGCG CG^CG PflMI 224 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 491 GAGTC GAGTC(4/5) PstI 457 CTGCAG CTGCA^G RcaI 389 TCATGA T^CATGA SfcI 457 CTGCAG C^T[GA][CT]AG SmaI 556 CCCGGG CCC^GGG Tsp509I 506 AATT ^AATT Van91I 224 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 556 CCCGGG C^CCGGG XmaI 556 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 221 GTAC GT^AC 576 GTAC AvaII 15 GGACC G^G[AT]CC 410 GGTCC BfaI 343 CTAG C^TAG 439 CTAG BpmI 414 CTCCAG CTGGAG(16/14) 187 CTGGAG BsmAI 282 GTCTC GTCTC(1/5) 606 GTCTC BsmFI 75 GGGAC GGGAC(10/14) 363 GTCCC BspMI 26 ACCTGC ACCTGC(4/8) 114 GCAGGT Cac8I 30 GCCTGC GC[ACGT]^[ACGT]GC 243 GCTTGC DraII 14 AGGACCC [GA]G^G[ACGT]CC[CT] 409 AGGTCCT EcoO109I 14 AGGACCC [GA]G^G[ACGT]CC[CT] 409 AGGTCCT FauI 173 CCCGC CCCGC(4/6) 318 GCGGG HaeIII 322 GGCC GG^CC 525 GGCC HgaI 203 GACGC GACGC(5/10) 176 GCGTC MaeI 343 CTAG C^TAG 439 CTAG MaeIII 333 GTCAC ^GT[ACGT]AC 512 GTGAC MslI 516 CATCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 593 CACAGGCATG PpuMI 14 AGGACCC [GA]G^G[AT]CC[CT] 409 AGGTCCT RsaI 221 GTAC GT^AC 576 GTAC SfaNI 240 GATGC GCATC(5/9) 431 GCATC TfiI 419 GAATC G^A[AT]TC 485 GATTC Tsp45I 333 GTCAC ^GT[GC]AC 512 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI AvaI BbvI BcnI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI Cfr13I EcoRII Fnu4HI FokI HapII HinfI HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI TspRI