(Linear) (Six-base) MAPSORT of: KIEE4022.seq Check: 3079 from: 1 to: 630 KIEE4022.orf With 182 enzymes: SgfI * March 22, 2012 17:10 .. Acc65I G'GTAC_C Cuts at: 0 575 630 Size: 575 55 AvaI C'yCGr_G Cuts at: 0 145 556 610 630 Size: 145 411 54 20 Fragments arranged by size: 411 145 54 20 BanI G'GyrC_C Cuts at: 0 575 630 Size: 575 55 Bme1580I G_kGCm'C Cuts at: 0 186 586 630 Size: 186 400 44 Fragments arranged by size: 400 186 44 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 399 630 Size: 208 191 231 Fragments arranged by size: 231 208 191 BseYI C'CCAG_C Cuts at: 0 85 630 Size: 85 545 BsiHKAI G_wGCw'C Cuts at: 0 271 630 Size: 271 359 Bsp1286I G_dGCh'C Cuts at: 0 186 271 586 630 Size: 186 85 315 44 Fragments arranged by size: 315 186 85 44 BspHI T'CATG_A Cuts at: 0 389 630 Size: 389 241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 35 105 630 Size: 35 70 525 Fragments arranged by size: 525 70 35 BssSI C'ACGA_G Cuts at: 0 520 630 Size: 520 110 BstAPI GCAn_nnn'nTGC Cuts at: 0 240 630 Size: 240 390 BtsI GCAGTG_nn' Cuts at: 0 101 426 630 Size: 101 325 204 Fragments arranged by size: 325 204 101 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 399 630 Size: 208 191 231 Fragments arranged by size: 231 208 191 EcoO109I rG'GnC_Cy Cuts at: 0 15 410 630 Size: 15 395 220 Fragments arranged by size: 395 220 15 HaeII r_GCGC'y Cuts at: 0 169 630 Size: 169 461 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 476 508 630 Size: 476 32 122 Fragments arranged by size: 476 122 32 KpnI G_GTAC'C Cuts at: 0 579 630 Size: 579 51 MslI CAynn'nnrTG Cuts at: 0 520 597 630 Size: 520 77 33 Fragments arranged by size: 520 77 33 PasI CC'CwG_GG Cuts at: 0 36 630 Size: 36 594 PflMI CCAn_nnn'nTGG Cuts at: 0 230 630 Size: 230 400 PpuMI rG'GwC_Cy Cuts at: 0 15 410 630 Size: 15 395 220 Fragments arranged by size: 395 220 15 PstI C_TGCA'G Cuts at: 0 461 630 Size: 461 169 SfcI C'TryA_G Cuts at: 0 457 630 Size: 457 173 SmaI CCC'GGG Cuts at: 0 558 630 Size: 558 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 591 630 Size: 591 39 XmaI C'CCGG_G Cuts at: 0 556 630 Size: 556 74 Enzymes that do cut: Acc65I AvaI BanI Bme1580I BpmI BseYI BsiHKAI Bsp1286I BspHI BspMI BssSI BstAPI BtsI Eco57MI EcoO109I HaeII Hin4I KpnI MslI PasI PflMI PpuMI PstI SfcI SmaI TaqII XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI