Restriction map for
pFN21AE1140
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BbeI BbrPI BbvCI BcgI BciVI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MslI MspA1I MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 106 AAATTC [GA]^AATT[CT]
AflII 49 CTTAAG C^TTAAG
AflIII 1021 ACATGT A^C[GA][CT]GT
Alw44I 862 GTGCAC G^TGCAC
AocI 835 CCTGAGG CC^T[ACGT]AGG
ApaI 424 GGGCCC GGGCC^C
ApaLI 862 GTGCAC G^TGCAC
ApoI 106 AAATTC [GA]^AATT[CT]
AspHI 862 GTGCAC G[AT]GC[AT]^C
AvrII 844 CCTAGG C^CTAGG
BalI 169 TGGCCA TGG^CCA
BamHI 1085 GGATCC G^GATCC
BanI 717 GGCACC G^G[CT][GA]CC
BbsI 1113 GAAGAC GAAGAC(2/6)
BclI 1016 TGATCA T^GATCA
BfrI 49 CTTAAG C^TTAAG
BlnI 844 CCTAGG C^CTAGG
BpuAI 1113 GAAGAC GAAGAC(2/6)
BsgI 1065 GTGCAG GTGCAG(16/14)
BsiEI 212 CGGCCG CG[GA][CT]^CG
BsiHKAI 862 GTGCAC G[AT]GC[AT]^C
BsmAI 58 GAGAC GTCTC(1/5)
BspLU11I 1021 ACATGT A^CATGT
BspMI 310 GCAGGT ACCTGC(4/8)
BsrDI 194 CATTGC GCAATG(2/0)
BstBI 251 TTCGAA TT^CGAA
Bsu36I 835 CCTGAGG CC^T[ACGT]AGG
DsaI 11 CCATGG C^C[GA][CT]GG
EagI 212 CGGCCG C^GGCCG
EclXI 212 CGGCCG C^GGCCG
Eco52I 212 CGGCCG C^GGCCG
Eco81I 835 CCTGAGG CC^T[ACGT]AGG
EcoNI 504 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 963 GCGGG CCCGC(4/6)
FbaI 1016 TGATCA T^GATCA
MfeI 89 CAATTG C^AATTG
MluNI 169 TGGCCA TGG^CCA
MscI 169 TGGCCA TGG^CCA
MunI 89 CAATTG C^AATTG
NcoI 11 CCATGG C^CATGG
NdeI 128 CATATG CA^TATG
NspI 1021 ACATGT [GA]CATG^[CT]
NspV 251 TTCGAA TT^CGAA
PshAI 1116 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 648 GAAGAGC GCTCTTC(1/4)
SfuI 251 TTCGAA TT^CGAA
StuI 601 AGGCCT AGG^CCT
Tsp45I 164 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BcnI 473 CCCGG CC^[GC]GG
1172 CCCGG
BfaI 149 CTAG C^TAG
845 CTAG
BglII 246 AGATCT A^GATCT
634 AGATCT
HapII 474 CCGG C^CGG
1173 CCGG
HpaII 474 CCGG C^CGG
1173 CCGG
HphI 802 GGTGA GGTGA(8/7)
997 GGTGA
MaeI 149 CTAG C^TAG
845 CTAG
MaeII 1081 ACGT A^CGT
1121 ACGT
MspI 474 CCGG C^CGG
1173 CCGG
NciI 473 CCCGG CC^[GC]GG
1172 CCCGG
PstI 111 CTGCAG CTGCA^G
355 CTGCAG
SfcI 111 CTGCAG C^T[GA][CT]AG
355 CTGCAG
TfiI 641 GAATC G^A[AT]TC
991 GAATC
Tsp509I 90 AATT ^AATT
107 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI Tru9I TseI TspRI
TthHB8I Van91I XhoII