Restriction map for
pFN21AE1140
(Circular) (Six-base) MAPSORT of: pFN21AE1140.seq Check: 6969 from: 1 to: 5914
pFN21AE1140 5914 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3257 1937
Size: 1320 4594
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2766 2895 3977 5361 5629 1632
Size: 1134 129 1082 1384 268 1917
Fragments arranged by size:
1917 1384 1134 1082 268 129
AflII C'TTAA_G
Cuts at: 820 1017 2054 820
Size: 197 1037 4680
Fragments arranged by size:
4680 1037 197
AflIII A'CryG_T
Cuts at: 1203 3026 4813 5790 1203
Size: 1823 1787 977 1327
Fragments arranged by size:
1823 1787 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5914
AlwNI CAG_nnn'CTG
Cuts at: 2183 2579 2744 2822 3657 5229 2183
Size: 396 165 78 835 1572 2868
Fragments arranged by size:
2868 1572 835 396 165 78
ApaI G_GGCC'C
Cuts at: 2433 2433
Size: 5914
ApaLI G'TGCA_C
Cuts at: 2867 3971 5127 2867
Size: 1104 1156 3654
Fragments arranged by size:
3654 1156 1104
ApoI r'AATT_y
Cuts at: 1493 2111 3223 3449 3601 5710 1493
Size: 618 1112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 3239 1870
Size: 73 1296 4545
Fragments arranged by size:
4545 1296 73
AvrII C'CTAG_G
Cuts at: 2849 2849
Size: 5914
BamHI G'GATC_C
Cuts at: 3090 3244 3611 3090
Size: 154 367 5393
Fragments arranged by size:
5393 367 154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2722 3235 4690 618
Size: 325 217 627 935 513 1455 1842
Fragments arranged by size:
1842 1455 935 627 513 325 217
BanII G_rGCy'C
Cuts at: 1391 2028 2433 3213 3233 1391
Size: 637 405 780 20 4072
Fragments arranged by size:
4072 780 637 405 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3125 928
Size: 971 1226 3717
Fragments arranged by size:
3717 1226 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4105 4139 1903
Size: 34 50 34 2084 34 3678
Fragments arranged by size:
3678 2084 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 3021 1548
Size: 1473 4441
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2251 2639 5909 2251
Size: 388 3270 2256
Fragments arranged by size:
3270 2256 388
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 2433 2508 2871 3975 5131 1790
Size: 643 75 363 1104 1156 2573
Fragments arranged by size:
2573 1156 1104 643 363 75
BmgBI CAC'GTC
Cuts at: 1318 3087 1318
Size: 1769 4145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2022 2724 2810
Size: 416 53 83 399 24 605 702 86
Cuts at: 2810 3645 4603 442
Size: 835 958 1753
Fragments arranged by size:
1753 958 835 702 605 416 399 86
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2935 3225 4574 1239
Size: 934 762 290 1349 2579
Fragments arranged by size:
2579 1349 934 762 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2295 2387 2408 4039 4904 5202 2295
Size: 92 21 1631 865 298 3007
Fragments arranged by size:
3007 1631 865 298 92 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4577 882
Size: 617 3078 2219
Fragments arranged by size:
3078 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 5908 5908
Size: 5914
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3721 4103 4738 275
Size: 53 83 186 936 2188 382 635 1451
Fragments arranged by size:
2188 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3198 3228 1178
Size: 30 165 30 1795 30 3864
Fragments arranged by size:
3864 1795 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2220 2526 2765 2768 2777 1186
Size: 1034 306 239 3 9 4323
Fragments arranged by size:
4323 1034 306 239 9 3
BseYI C'CCAG_C
Cuts at: 2232 2325 2579 5117 5613 2232
Size: 93 254 2538 496 2533
Fragments arranged by size:
2538 2533 496 254 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3091 1163
Size: 573 1355 3986
Fragments arranged by size:
3986 1355 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2220 3364 4125 5153 1540
Size: 275 186 219 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 219 186
BsiHKAI G_wGCw'C
Cuts at: 2871 3975 4060 5131 2871
Size: 1104 85 1071 3654
Fragments arranged by size:
3654 1104 1071 85
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2433 2508 2871 3213 3233 3975
Size: 399 238 405 75 363 342 20 742
Cuts at: 3975 4060 5131 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 363 342 238
85 75 20
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 3269 844
Size: 1462 963 3489
Fragments arranged by size:
3489 1462 963
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2198 4409 4583 65
Size: 1183 950 2211 174 1396
Fragments arranged by size:
2211 1396 1183 950 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 2257 4718 2257
Size: 2461 3453
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2038 2251 2639 3016 3090 3244 3611 3996
Size: 66 213 388 377 74 154 367 385
Cuts at: 3996 4013 5465 5551 5563 5909 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 388 385 377 367 346 213
154 86 74 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 2841 2841
Size: 5914
BtgI C'CryG_G
Cuts at: 513 1065 2016 513
Size: 552 951 4411
Fragments arranged by size:
4411 951 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 3430 4224 4244 3430
Size: 794 20 5100
Fragments arranged by size:
5100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2174 2217 2612
Size: 54 1425 50 275 18 344 43 395
Cuts at: 2612 3361 4250 5879 8
Size: 749 889 1629 43
Fragments arranged by size:
1629 1425 889 749 395 344 275 54
50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2217 3361 1537
Size: 275 405 1144 4090
Fragments arranged by size:
4090 1144 405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2648 2742 2754 2763 2778 3844 1353
Size: 1295 94 12 9 15 1066 3423
Fragments arranged by size:
3423 1295 1066 94 15 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 2101 4501 4875 5021 387
Size: 1064 138 447 65 2400 374 146 1280
Fragments arranged by size:
2400 1280 1064 447 374 146 138 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2766 2895 2935 3225 3977 4574
Size: 393 541 593 129 40 290 752 597
Cuts at: 4574 5361 5629 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 752 597 593 541 393 290
268 129 40
EcoNI CCTnn'n_nnAGG
Cuts at: 2513 2513
Size: 5914
EcoO109I rG'GnC_Cy
Cuts at: 2318 2333 2365 2521 2853 3191 2318
Size: 15 32 156 332 338 5041
Fragments arranged by size:
5041 338 332 156 32 15
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2364 2364
Size: 5914
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 5061 1678
Size: 3383 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2631 2663 2726 2758 2933
Size: 32 135 32 1059 32 63 32 175
Cuts at: 2933 2965 4555 4587 4629 4661 1373
Size: 32 1590 32 42 32 2626
Fragments arranged by size:
2626 1590 1059 175 135 63 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 2094 3513 2094
Size: 1419 4495
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2072 5028 5212 1305
Size: 767 2956 184 2007
Fragments arranged by size:
2956 2007 767 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2176 10
Size: 54 1425 343 344 3748
Fragments arranged by size:
3748 1425 344 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3872 4231 4390 518
Size: 648 2706 359 159 2042
Fragments arranged by size:
2706 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3330 4007 5155 5400 1606
Size: 1724 677 1148 245 2120
Fragments arranged by size:
2120 1724 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2016 513
Size: 552 951 4411
Fragments arranged by size:
4411 951 552
NdeI CA'TA_TG
Cuts at: 387 2134 387
Size: 1747 4167
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 3030 3272 4817 1132
Size: 97 1801 242 1545 2229
Fragments arranged by size:
2229 1801 1545 242 97
PasI CC'CwG_GG
Cuts at: 1348 2515 1348
Size: 1167 4747
PciI A'CATG_T
Cuts at: 3026 4813 3026
Size: 1787 4127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2183 2579 2621 2708 1108
Size: 1075 396 42 87 4314
Fragments arranged by size:
4314 1075 396 87 42
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2318 2333 2521 2318
Size: 15 188 5711
Fragments arranged by size:
5711 188 15
PshAI GACnn'nnGTC
Cuts at: 1613 3125 1613
Size: 1512 4402
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PspOMI G'GGCC_C
Cuts at: 2429 2429
Size: 5914
PstI C_TGCA'G
Cuts at: 830 2120 2364 3266 830
Size: 1290 244 902 3478
Fragments arranged by size:
3478 1290 902 244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SapI GCTCTTCn'nnn_
Cuts at: 2648 2648
Size: 5914
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2116 2360 3262 4397 5078 5269 826
Size: 219 1071 244 902 1135 681 191 1471
Fragments arranged by size:
1471 1135 1071 902 681 244 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2310 2387 2402 4018 4919
Size: 197 926 111 256 77 15 1616 901
Cuts at: 4919 5181 820
Size: 262 1553
Fragments arranged by size:
1616 1553 926 901 262 256 197 111
77 15
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 3838 5
Size: 47 3786 2081
Fragments arranged by size:
3786 2081 47
StuI AGG'CCT
Cuts at: 1517 2608 1517
Size: 1091 4823
StyI C'CwwG_G
Cuts at: 513 1065 2016 2339 2849 3353 513
Size: 552 951 323 510 504 3074
Fragments arranged by size:
3074 951 552 510 504 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 2565 3941 3958 4111 4296 4741
Size: 1255 759 226 1376 17 153 185 445
Cuts at: 4741 325
Size: 1498
Fragments arranged by size:
1498 1376 1255 759 445 226 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4160 5872 96
Size: 274 80 33 51 1443 2183 1712 138
Fragments arranged by size:
2183 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 4043 4043
Size: 5914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI