Restriction map for
pFN21AE1140
(Linear) (Six-base) MAPSORT of: KIEE1140.seq Check: 9034 from: 1 to: 1209
KIEE1140.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 890 1209
Size: 761 129 319
Fragments arranged by size:
761 319 129
AflII C'TTAA_G
Cuts at: 0 49 1209
Size: 49 1160
AflIII A'CryG_T
Cuts at: 0 1021 1209
Size: 1021 188
AlwNI CAG_nnn'CTG
Cuts at: 0 178 574 739 817 1209
Size: 178 396 165 78 392
Fragments arranged by size:
396 392 178 165 78
ApaI G_GGCC'C
Cuts at: 0 428 1209
Size: 428 781
ApaLI G'TGCA_C
Cuts at: 0 862 1209
Size: 862 347
ApoI r'AATT_y
Cuts at: 0 106 1209
Size: 106 1103
AvrII C'CTAG_G
Cuts at: 0 844 1209
Size: 844 365
BamHI G'GATC_C
Cuts at: 0 1085 1209
Size: 1085 124
BanI G'GyrC_C
Cuts at: 0 717 1209
Size: 717 492
BanII G_rGCy'C
Cuts at: 0 23 428 1208 1209
Size: 23 405 780 1
Fragments arranged by size:
780 405 23 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 1120 1209
Size: 1120 89
BclI T'GATC_A
Cuts at: 0 1016 1209
Size: 1016 193
BglII A'GATC_T
Cuts at: 0 246 634 1209
Size: 246 388 575
Fragments arranged by size:
575 388 246
Bme1580I G_kGCm'C
Cuts at: 0 428 503 866 1209
Size: 428 75 363 343
Fragments arranged by size:
428 363 343 75
BmgBI CAC'GTC
Cuts at: 0 1082 1209
Size: 1082 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 17 719 805 1209
Size: 17 702 86 404
Fragments arranged by size:
702 404 86 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 930 1209
Size: 168 762 279
Fragments arranged by size:
762 279 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 382 403 1209
Size: 290 92 21 806
Fragments arranged by size:
806 290 92 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 215 521 760 763 772 1209
Size: 215 306 239 3 9 437
Fragments arranged by size:
437 306 239 215 9 3
BseYI C'CCAG_C
Cuts at: 0 227 320 574 1209
Size: 227 93 254 635
Fragments arranged by size:
635 254 227 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1209
Size: 1086 123
BsiEI CG_ry'CG
Cuts at: 0 215 1209
Size: 215 994
BsiHKAI G_wGCw'C
Cuts at: 0 866 1209
Size: 866 343
Bsp1286I G_dGCh'C
Cuts at: 0 23 428 503 866 1208 1209
Size: 23 405 75 363 342 1
Fragments arranged by size:
405 363 342 75 23 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 301 1209
Size: 301 908
BsrDI GCAATG_nn'
Cuts at: 0 193 1209
Size: 193 1016
BstBI TT'CG_AA
Cuts at: 0 252 1209
Size: 252 957
BstYI r'GATC_y
Cuts at: 0 33 246 634 1011 1085 1209
Size: 33 213 388 377 74 124
Fragments arranged by size:
388 377 213 124 74 33
Bsu36I CC'TnA_GG
Cuts at: 0 836 1209
Size: 836 373
BtgI C'CryG_G
Cuts at: 0 11 1209
Size: 11 1198
EaeI y'GGCC_r
Cuts at: 0 169 212 607 1209
Size: 169 43 395 602
Fragments arranged by size:
602 395 169 43
EagI C'GGCC_G
Cuts at: 0 212 1209
Size: 212 997
EarI CTCTTCn'nnn_
Cuts at: 0 643 737 749 758 773 1209
Size: 643 94 12 9 15 436
Fragments arranged by size:
643 436 94 15 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 96 1209
Size: 31 65 1113
Fragments arranged by size:
1113 65 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 761 890 930 1209
Size: 168 593 129 40 279
Fragments arranged by size:
593 279 168 129 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 508 1209
Size: 508 701
EcoO109I rG'GnC_Cy
Cuts at: 0 313 328 360 516 848 1186 1209
Size: 313 15 32 156 332 338 23
Fragments arranged by size:
338 332 313 156 32 23 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 359 1209
Size: 359 850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 626 658 721 753 928 960 1209
Size: 626 32 63 32 175 32 249
Fragments arranged by size:
626 249 175 63 32 32 32
MfeI C'AATT_G
Cuts at: 0 89 1209
Size: 89 1120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1209
Size: 67 1142
MscI TGG'CCA
Cuts at: 0 171 1209
Size: 171 1038
NcoI C'CATG_G
Cuts at: 0 11 1209
Size: 11 1198
NdeI CA'TA_TG
Cuts at: 0 129 1209
Size: 129 1080
NspI r_CATG'y
Cuts at: 0 1025 1209
Size: 1025 184
PasI CC'CwG_GG
Cuts at: 0 510 1209
Size: 510 699
PciI A'CATG_T
Cuts at: 0 1021 1209
Size: 1021 188
PflMI CCAn_nnn'nTGG
Cuts at: 0 178 574 616 703 1209
Size: 178 396 42 87 506
Fragments arranged by size:
506 396 178 87 42
PpuMI rG'GwC_Cy
Cuts at: 0 313 328 516 1209
Size: 313 15 188 693
Fragments arranged by size:
693 313 188 15
PshAI GACnn'nnGTC
Cuts at: 0 1120 1209
Size: 1120 89
PspOMI G'GGCC_C
Cuts at: 0 424 1209
Size: 424 785
PstI C_TGCA'G
Cuts at: 0 115 359 1209
Size: 115 244 850
Fragments arranged by size:
850 244 115
SapI GCTCTTCn'nnn_
Cuts at: 0 643 1209
Size: 643 566
SfcI C'TryA_G
Cuts at: 0 111 355 1209
Size: 111 244 854
Fragments arranged by size:
854 244 111
SmlI C'TyrA_G
Cuts at: 0 49 305 382 397 1209
Size: 49 256 77 15 812
Fragments arranged by size:
812 256 77 49 15
StuI AGG'CCT
Cuts at: 0 603 1209
Size: 603 606
StyI C'CwwG_G
Cuts at: 0 11 334 844 1209
Size: 11 323 510 365
Fragments arranged by size:
510 365 323 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 560 1209
Size: 334 226 649
Fragments arranged by size:
649 334 226
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApaI ApaLI ApoI AvrII
BamHI BanI BanII BbsI BclI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrDI BstBI BstYI Bsu36I BtgI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I
MfeI MmeI MscI NcoI NdeI NspI PasI PciI
PflMI PpuMI PshAI PspOMI PstI SapI SfcI SmlI
StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI BaeI
BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MslI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI