################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcyI 310 GACGCC G[GA]^CG[CT]C AfaI 204 GTAC GT^AC AflIII 745 ACGTGT A^C[GA][CT]GT AlwNI 26 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG BanII 181 GAGCCC G[GA]GC[CT]^C BbvCI 153 CCTCAGC CCTCAGC(-5/-2) BfaI 76 CTAG C^TAG BpmI 594 CTCCAG CTGGAG(16/14) BsaHI 310 GACGCC G[GA]^CG[CT]C BsmBI 277 CGTCTC CGTCTC(1/5) BsrDI 265 GCAATG GCAATG(2/0) DpnII 953 GATC ^GATC DraIII 502 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG FauI 428 CCCGC CCCGC(4/6) Hin1I 310 GACGCC G[GA]^CG[CT]C MaeI 76 CTAG C^TAG MboI 953 GATC ^GATC MslI 426 CACCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 377 CCATGG C^CATGG NdeII 953 GATC ^GATC PstI 695 CTGCAG CTGCA^G PvuII 692 CAGCTG CAG^CTG RsaI 204 GTAC GT^AC Sau3AI 953 GATC ^GATC SspI 936 AATATT AAT^ATT StuI 734 AGGCCT AGG^CCT TfiI 707 GAATC G^A[AT]TC Tsp509I 300 AATT ^AATT XcmI 339 CCACCTTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 707 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 732 GAAGGCCTTC AspHI 112 GTGCTC G[AT]GC[AT]^C 923 GTGCTC AvaII 35 GGACC G^G[AT]CC 44 GGACC BalI 398 TGGCCA TGG^CCA 509 TGGCCA BbrPI 685 CACGTG CAC^GTG 744 CACGTG BsaAI 685 CACGTG [CT]AC^GT[GA] 744 CACGTG BsiHKAI 112 GTGCTC G[AT]GC[AT]^C 923 GTGCTC BsmAI 807 GAGAC GTCTC(1/5) 278 GTCTC BsmFI 34 GGGAC GGGAC(10/14) 285 GTCCC DsaI 377 CCATGG C^C[GA][CT]GG 764 CCGTGG EaeI 398 TGGCCA [CT]^GGCC[GA] 509 TGGCCA EcoT14I 377 CCATGG C^C[AT][AT]GG 470 CCTTGG HgaI 310 GACGC GACGC(5/10) 466 GCGTC MaeII 686 ACGT A^CGT 745 ACGT MluNI 398 TGGCCA TGG^CCA 509 TGGCCA MscI 398 TGGCCA TGG^CCA 509 TGGCCA MspA1I 47 CCGCTG C[AC]G^C[GT]G 692 CAGCTG PleI 392 GACTC GAGTC(4/5) 435 GAGTC PmaCI 685 CACGTG CAC^GTG 744 CACGTG PmlI 685 CACGTG CAC^GTG 744 CACGTG SapI 327 GCTCTTC GCTCTTC(1/4) 576 GCTCTTC SfaNI 93 GATGC GCATC(5/9) 158 GCATC StyI 377 CCATGG C^C[AT][AT]GG 470 CCTTGG XmnI 707 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 732 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV PflMI Sau96I ScrFI SfcI TseI Tsp45I TspRI Van91I