Restriction map for
pFN21AE1987
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HindIII
KpnI KspI MamI MluI MroI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfcI SfiI SfuI
SgrAI SmaI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1201 GACGTC GACGT^C
AccI 704 GTCTAC GT^[AC][GT]AC
AclI 1561 AACGTT AA^CGTT
AflIII 166 ACGTGT A^C[GA][CT]GT
Asp700I 1039 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1265 TGGCCA TGG^CCA
BanI 765 GGCGCC G^G[CT][GA]CC
BbeI 765 GGCGCC GGCGC^C
BcnI 1377 CCCGG CC^[GC]GG
BglII 270 AGATCT A^GATCT
BsaAI 1652 TACGTA [CT]AC^GT[GA]
BseRI 778 GAGGAG GAGGAG(10/8)
BstEII 280 GGTCACC G^GT[ACGT]ACC
BstPI 280 GGTCACC G^GT[ACGT]ACC
BstXI 566 CCAATCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 136 TTTAAA TTT^AAA
DsaI 1385 CCACGG C^C[GA][CT]GG
EcoNI 1359 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 280 GGTCACC G^GT[ACGT]ACC
EcoRI 820 GAATTC G^AATTC
EcoT22I 19 ATGCAT ATGCA^T
FauI 1308 GCGGG CCCGC(4/6)
HaeII 765 GGCGCC [GA]GCGC^[CT]
HgaI 952 GCGTC GACGC(5/10)
HincII 1681 GTTAAC GT[CT]^[GA]AC
HindII 1681 GTTAAC GT[CT]^[GA]AC
HpaI 1681 GTTAAC GTT^AAC
KasI 765 GGCGCC G^GCGCC
MfeI 571 CAATTG C^AATTG
MluNI 1265 TGGCCA TGG^CCA
MscI 1265 TGGCCA TGG^CCA
MslI 462 CATAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 571 CAATTG C^AATTG
NarI 765 GGCGCC GG^CGCC
NciI 1377 CCCGG CC^[GC]GG
NsiI 19 ATGCAT ATGCA^T
PflMI 1257 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1285 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1561 AACGTT AA^CGTT
PvuII 1171 CAGCTG CAG^CTG
ScaI 301 AGTACT AGT^ACT
SfoI 765 GGCGCC GGC^GCC
SmlI 240 CTTGAG C^T[CT][GA]AG
SnaBI 1652 TACGTA TAC^GTA
StuI 1211 AGGCCT AGG^CCT
TaqI 606 TCGA T^CGA
TthHB8I 606 TCGA T^CGA
Van91I 1257 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 932 CCATAACATTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1039 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 689 GGATC GGATC(4/5)
1623 GGATC
AlwNI 1496 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1537 CAGTACCTG
AocI 177 CCTAAGG CC^T[ACGT]AGG
1195 CCTGAGG
ApaI 1272 GGGCCC GGGCC^C
1335 GGGCCC
AspHI 158 GAGCTC G[AT]GC[AT]^C
1190 GAGCTC
AvaI 1187 CCCGAG C^[CT]CG[GA]G
1344 CCCGAG
BbsI 486 GAAGAC GAAGAC(2/6)
608 GAAGAC
BfaI 620 CTAG C^TAG
1448 CTAG
BpuAI 486 GAAGAC GAAGAC(2/6)
608 GAAGAC
BsiHKAI 158 GAGCTC G[AT]GC[AT]^C
1190 GAGCTC
BsmBI 1516 CGTCTC CGTCTC(1/5)
1000 GAGACG
BsmFI 1356 GTCCC GGGAC(10/14)
1362 GTCCC
BsoBI 1187 CCCGAG C^[CT]CG[GA]G
1344 CCCGAG
BstYI 270 AGATCT [GA]^GATC[CT]
1623 GGATCT
Bsu36I 177 CCTAAGG CC^T[ACGT]AGG
1195 CCTGAGG
CfoI 766 GCGC GCG^C
993 GCGC
Eco81I 177 CCTAAGG CC^T[ACGT]AGG
1195 CCTGAGG
EcoT14I 581 CCAAGG C^C[AT][AT]GG
1214 CCTTGG
HapII 1378 CCGG C^CGG
1532 CCGG
HhaI 766 GCGC GCG^C
993 GCGC
HinP1I 766 GCGC G^CGC
993 GCGC
HpaII 1378 CCGG C^CGG
1532 CCGG
MaeI 620 CTAG C^TAG
1448 CTAG
MflI 270 AGATCT [GA]^GATC[CT]
1623 GGATCT
MspA1I 1171 CAGCTG C[AC]G^C[GT]G
1325 CCGCTG
MspI 1378 CCGG C^CGG
1532 CCGG
SacI 158 GAGCTC GAGCT^C
1190 GAGCTC
SphI 21 GCATGC GCATG^C
740 GCATGC
StyI 581 CCAAGG C^C[AT][AT]GG
1214 CCTTGG
XhoII 270 AGATCT [GA]^GATC[CT]
1623 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
ApoI AvaII BanII BbvI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII Hin1I HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI