Restriction map for
pFN21AE1987
(Circular) (Six-base) MAPSORT of: pFN21AE1987.seq Check: 9567 from: 1 to: 6391
pFN21AE1987 6391 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3210 4201 278
Size: 53 83 186 936 1674 991 2468
Fragments arranged by size:
2468 1674 991 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2710 3734 1937
Size: 773 1024 4594
Fragments arranged by size:
4594 1024 773
Acc65I G'GTAC_C
Cuts at: 3712 3712
Size: 6391
AclI AA'CG_TT
Cuts at: 1563 3567 3690 4518 4891 1563
Size: 2004 123 828 373 3063
Fragments arranged by size:
3063 2004 828 373 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2798 4454 5838 6106 1632
Size: 1166 1656 1384 268 1917
Fragments arranged by size:
1917 1656 1384 1166 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 2171 5290 6267 1203
Size: 968 3119 977 1327
Fragments arranged by size:
3119 1327 977 968
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6391
AlwNI CAG_nnn'CTG
Cuts at: 3506 3547 4134 5706 3506
Size: 41 587 1572 4191
Fragments arranged by size:
4191 1572 587 41
ApaI G_GGCC'C
Cuts at: 3281 3344 3281
Size: 63 6328
ApaLI G'TGCA_C
Cuts at: 4448 5604 4448
Size: 1156 5235
ApoI r'AATT_y
Cuts at: 1493 2825 3048 3666 3700 3926 4078 6187 1493
Size: 1332 223 618 34 226 152 2109 1697
Fragments arranged by size:
2109 1697 1332 618 226 223 152 34
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3192 3349 3716 1870
Size: 73 1249 157 367 4545
Fragments arranged by size:
4545 1249 367 157 73
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2770 3712 5167 618
Size: 325 217 627 983 942 1455 1842
Fragments arranged by size:
1842 1455 983 942 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2167 3199 3281 3344 3466 3710 1391
Size: 776 1032 82 63 122 244 4072
Fragments arranged by size:
4072 1032 776 244 122 82 63
BbeI G_GCGC'C
Cuts at: 2774 2774
Size: 6391
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2498 2620 928
Size: 971 599 122 4699
Fragments arranged by size:
4699 971 599 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4582 4616 1903
Size: 34 2645 34 3678
Fragments arranged by size:
3678 2645 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4283 5493 4283
Size: 1210 5181
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BfrBI ATG'CAT
Cuts at: 2026 2026
Size: 6391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5002 136
Size: 107 122 71 631 605 3330 1525
Fragments arranged by size:
3330 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2275 6386 2275
Size: 4111 2280
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 3281 3344 3350 4452 5608 1790
Size: 1491 63 6 1102 1156 2573
Fragments arranged by size:
2573 1491 1156 1102 63 6
BmgBI CAC'GTC
Cuts at: 1318 2172 1318
Size: 854 5537
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 3494 3616
Size: 416 53 83 399 24 858 1219 122
Cuts at: 3616 4122 5080 442
Size: 506 958 1753
Fragments arranged by size:
1753 1219 958 858 506 416 399 122
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5051 1239
Size: 3812 2579
Bpu10I CC'TnA_GC
Cuts at: 2857 2857
Size: 6391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2266 4516 5381 5679 2266
Size: 2250 865 298 2978
Fragments arranged by size:
2978 2250 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5054 882
Size: 617 3555 2219
Fragments arranged by size:
3555 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3659 4182 493
Size: 711 2455 523 2702
Fragments arranged by size:
2702 2455 711 523
BsaBI GATnn'nnATC
Cuts at: 6385 6385
Size: 6391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2771 2957 3207 4198
Size: 53 83 186 936 1238 186 250 991
Cuts at: 4198 4580 5215 275
Size: 382 635 1451
Fragments arranged by size:
1451 1238 991 936 635 382 250 186
186 83 53
BsaWI w'CCGG_w
Cuts at: 4824 5222 5496 5643 6054 4824
Size: 398 274 147 411 5161
Fragments arranged by size:
5161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3329 3350 3359 3380 1178
Size: 30 165 30 1926 21 9 21 4189
Fragments arranged by size:
4189 1926 165 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2798 1186
Size: 1612 4779
BseYI C'CCAG_C
Cuts at: 2749 5594 6090 2749
Size: 2845 496 3050
Fragments arranged by size:
3050 2845 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5818
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3841 4602 5630 1540
Size: 275 186 1840 761 1028 2301
Fragments arranged by size:
2301 1840 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2167 3199 4452 4537 5608 2167
Size: 1032 1253 85 1071 2950
Fragments arranged by size:
2950 1253 1071 1032 85
BsmI GAATG_Cn'
Cuts at: 3902 3995 3902
Size: 93 6298
BsmBI CGTCTCn'nnnn_
Cuts at: 2999 3527 2999
Size: 528 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2167 3199 3281 3344 3350 3466 3710
Size: 399 377 1032 82 63 6 116 244
Cuts at: 3710 4452 4537 5608 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 742 399 377 244 116
85 82 63 6
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3746 844
Size: 2902 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4278 1394
Size: 429 2455 3507
Fragments arranged by size:
3507 2455 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4886 5060 65
Size: 1183 3638 174 1396
Fragments arranged by size:
3638 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5035 5222 1700
Size: 110 3225 187 2869
Fragments arranged by size:
3225 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6391
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 2285 1184
Size: 1101 5290
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 2578
Size: 6391
BstYI r'GATC_y
Cuts at: 1972 2275 3628 3721 4088 4473 4490 5942 6028
Size: 303 1353 93 367 385 17 1452 86
Cuts at: 6028 6040 6386 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1353 385 367 346 303 93
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2183 3201 2183
Size: 1018 5373
BtgI C'CryG_G
Cuts at: 513 1065 2004 3390 513
Size: 552 939 1386 3514
Fragments arranged by size:
3514 1386 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5652
Fragments arranged by size:
5652 683 56
BtsI GCAGTG_nn'
Cuts at: 3907 4701 4721 3907
Size: 794 20 5577
Fragments arranged by size:
5577 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2385 2420 529
Size: 35 1821 35 4500
Fragments arranged by size:
4500 1821 35 35
DraI TTT'AAA
Cuts at: 2143 3695 2143
Size: 1552 4839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5398 809
Size: 507 4082 1802
Fragments arranged by size:
4082 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3120 3270 3534
Size: 54 1425 50 275 18 1290 150 264
Cuts at: 3534 3838 4727 6356 8
Size: 304 889 1629 43
Fragments arranged by size:
1629 1425 1290 889 304 275 264 150
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3838 1537
Size: 275 2026 4090
Fragments arranged by size:
4090 2026 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2119 2311 3219 4321 1353
Size: 766 192 908 1102 3423
Fragments arranged by size:
3423 1102 908 766 192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2610 3026 4978 5352 5498 387
Size: 1064 138 1021 416 1952 374 146 1280
Fragments arranged by size:
1952 1280 1064 1021 416 374 146 138
EcoICRI GAG'CTC
Cuts at: 2165 3197 2165
Size: 1032 5359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2798 4454 5051 5838 6106 1239
Size: 393 1166 1656 597 787 268 1524
Fragments arranged by size:
1656 1524 1166 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3368 3368
Size: 6391
EcoO109I rG'GnC_Cy
Cuts at: 3277 3278 3291 3340 3277
Size: 1 13 49 6328
Fragments arranged by size:
6328 49 13 1
EcoRI G'AATT_C
Cuts at: 2825 3700 4078 2825
Size: 875 378 5138
Fragments arranged by size:
5138 875 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3125 3125
Size: 6391
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 2774 5538 1678
Size: 1096 2764 2531
Fragments arranged by size:
2764 2531 1096
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2494 2526 3350
Size: 32 135 32 589 32 301 32 824
Cuts at: 3350 3382 5032 5064 5106 5138 1373
Size: 32 1650 32 42 32 2626
Fragments arranged by size:
2626 1650 824 589 301 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3688 3735 3981 669
Size: 988 281 1750 47 246 3079
Fragments arranged by size:
3079 1750 988 281 246 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3688 3981 3688
Size: 293 6098
KasI G'GCGC_C
Cuts at: 2770 2770
Size: 6391
KpnI G_GTAC'C
Cuts at: 3716 3716
Size: 6391
MfeI C'AATT_G
Cuts at: 2576 3990 2576
Size: 1414 4977
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2819 5505 5689 1305
Size: 1514 2686 184 2007
Fragments arranged by size:
2686 2007 1514 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3272 10
Size: 54 1425 343 1440 3129
Fragments arranged by size:
3129 1440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2471 4349 4708 4867 518
Size: 648 1305 1878 359 159 2042
Fragments arranged by size:
2042 1878 1305 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3178 3332 3807 4484 5632 5877 1606
Size: 1572 154 475 677 1148 245 2120
Fragments arranged by size:
2120 1572 1148 677 475 245 154
NarI GG'CG_CC
Cuts at: 2771 2771
Size: 6391
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4900
Fragments arranged by size:
4900 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NsiI A_TGCA'T
Cuts at: 2028 2028
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 2030 2105 2551 2749 3749 5294 1132
Size: 97 801 75 446 198 1000 1545 2229
Fragments arranged by size:
2229 1545 1000 801 446 198 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3268 1108
Size: 2160 4231
PfoI T'CCnGG_A
Cuts at: 3134 3134
Size: 6391
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3291 3291
Size: 6391
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PspOMI G'GGCC_C
Cuts at: 3277 3340 3277
Size: 63 6328
PstI C_TGCA'G
Cuts at: 830 3743 830
Size: 2913 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 3178 3178
Size: 6391
SacI G_AGCT'C
Cuts at: 2167 3199 2167
Size: 1032 5359
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 2308 4639 6351 2308
Size: 2331 1712 2348
Fragments arranged by size:
2348 2331 1712
SfcI C'TryA_G
Cuts at: 826 1045 3739 4874 5555 5746 826
Size: 219 2694 1135 681 191 1471
Fragments arranged by size:
2694 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2772 2772
Size: 6391
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3718 1872
Size: 1846 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2245 4495 5396 5658 820
Size: 197 926 302 2250 901 262 1553
Fragments arranged by size:
2250 1553 926 901 302 262 197
SnaBI TAC'GTA
Cuts at: 493 3659 4182 493
Size: 3166 523 2702
Fragments arranged by size:
3166 2702 523
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 2030 2749 3749 1132
Size: 898 719 1000 3774
Fragments arranged by size:
3774 1000 898 719
SspI AAT'ATT
Cuts at: 5 52 4315 5
Size: 47 4263 2081
Fragments arranged by size:
4263 2081 47
StuI AGG'CCT
Cuts at: 1517 3218 1517
Size: 1701 4690
StyI C'CwwG_G
Cuts at: 513 1065 2004 2586 3219 3830 513
Size: 552 939 582 633 611 3074
Fragments arranged by size:
3074 939 633 611 582 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2273 4418 4435 4588 4773 5218 325
Size: 1255 693 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 693 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2174 2306 4637
Size: 274 80 33 51 1443 197 132 2331
Cuts at: 4637 6349 96
Size: 1712 138
Fragments arranged by size:
2331 1712 1443 274 197 138 132 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 3283 3315 3329 3361 6351 6383 2399
Size: 32 852 32 14 32 2990 32 2407
Fragments arranged by size:
2990 2407 852 32 32 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6391
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2944 2944
Size: 6391
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3716 1870
Size: 1846 4545
XmnI GAAnn'nnTTC
Cuts at: 3048 4520 3048
Size: 1472 4919
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3208 4199 276
Size: 53 83 186 936 1674 991 2468
Fragments arranged by size:
2468 1674 991 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI