(Linear) (Six-base) MAPSORT of: KIEE1987.seq Check: 4861 from: 1 to: 1686 KIEE1987.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AatII G_ACGT'C Cuts at: 0 1205 1686 Size: 1205 481 AccI GT'mk_AC Cuts at: 0 705 1686 Size: 705 981 AclI AA'CG_TT Cuts at: 0 1562 1686 Size: 1562 124 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 1686 Size: 793 893 AflIII A'CryG_T Cuts at: 0 166 1686 Size: 166 1520 AlwNI CAG_nnn'CTG Cuts at: 0 1501 1542 1686 Size: 1501 41 144 Fragments arranged by size: 1501 144 41 ApaI G_GGCC'C Cuts at: 0 1276 1339 1686 Size: 1276 63 347 Fragments arranged by size: 1276 347 63 ApoI r'AATT_y Cuts at: 0 820 1043 1661 1686 Size: 820 223 618 25 Fragments arranged by size: 820 618 223 25 AvaI C'yCGr_G Cuts at: 0 1187 1344 1686 Size: 1187 157 342 Fragments arranged by size: 1187 342 157 BanI G'GyrC_C Cuts at: 0 765 1686 Size: 765 921 BanII G_rGCy'C Cuts at: 0 162 1194 1276 1339 1461 1686 Size: 162 1032 82 63 122 225 Fragments arranged by size: 1032 225 162 122 82 63 BbeI G_GCGC'C Cuts at: 0 769 1686 Size: 769 917 BbsI GAAGACnn'nnnn_ Cuts at: 0 493 615 1686 Size: 493 122 1071 Fragments arranged by size: 1071 493 122 BfrBI ATG'CAT Cuts at: 0 21 1686 Size: 21 1665 BglII A'GATC_T Cuts at: 0 270 1686 Size: 270 1416 Bme1580I G_kGCm'C Cuts at: 0 1276 1339 1345 1686 Size: 1276 63 6 341 Fragments arranged by size: 1276 341 63 6 BmgBI CAC'GTC Cuts at: 0 167 1686 Size: 167 1519 BmrI ACTGGGnnnn_n' Cuts at: 0 270 1489 1611 1686 Size: 270 1219 122 75 Fragments arranged by size: 1219 270 122 75 Bpu10I CC'TnA_GC Cuts at: 0 852 1686 Size: 852 834 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 261 1686 Size: 261 1425 BsaAI yAC'GTr Cuts at: 0 1654 1686 Size: 1654 32 BsaHI Gr'CG_yC Cuts at: 0 766 952 1202 1686 Size: 766 186 250 484 Fragments arranged by size: 766 484 250 186 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1324 1345 1354 1375 1686 Size: 1324 21 9 21 311 Fragments arranged by size: 1324 311 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 793 1686 Size: 793 893 BseYI C'CCAG_C Cuts at: 0 744 1686 Size: 744 942 BsiHKAI G_wGCw'C Cuts at: 0 162 1194 1686 Size: 162 1032 492 Fragments arranged by size: 1032 492 162 BsmBI CGTCTCn'nnnn_ Cuts at: 0 994 1522 1686 Size: 994 528 164 Fragments arranged by size: 994 528 164 Bsp1286I G_dGCh'C Cuts at: 0 162 1194 1276 1339 1345 1461 1686 Size: 162 1032 82 63 6 116 225 Fragments arranged by size: 1032 225 162 116 82 63 6 BstEII G'GTnAC_C Cuts at: 0 280 1686 Size: 280 1406 BstXI CCAn_nnnn'nTGG Cuts at: 0 573 1686 Size: 573 1113 BstYI r'GATC_y Cuts at: 0 270 1623 1686 Size: 270 1353 63 Fragments arranged by size: 1353 270 63 Bsu36I CC'TnA_GG Cuts at: 0 178 1196 1686 Size: 178 1018 490 Fragments arranged by size: 1018 490 178 BtgI C'CryG_G Cuts at: 0 1385 1686 Size: 1385 301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 380 415 1686 Size: 380 35 1271 Fragments arranged by size: 1271 380 35 DraI TTT'AAA Cuts at: 0 138 1686 Size: 138 1548 EaeI y'GGCC_r Cuts at: 0 1115 1265 1529 1686 Size: 1115 150 264 157 Fragments arranged by size: 1115 264 157 150 EarI CTCTTCn'nnn_ Cuts at: 0 114 306 1214 1686 Size: 114 192 908 472 Fragments arranged by size: 908 472 192 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 605 1021 1686 Size: 605 416 665 Fragments arranged by size: 665 605 416 EcoICRI GAG'CTC Cuts at: 0 160 1192 1686 Size: 160 1032 494 Fragments arranged by size: 1032 494 160 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 1686 Size: 793 893 EcoNI CCTnn'n_nnAGG Cuts at: 0 1363 1686 Size: 1363 323 EcoO109I rG'GnC_Cy Cuts at: 0 1272 1273 1286 1335 1686 Size: 1272 1 13 49 351 Fragments arranged by size: 1272 351 49 13 1 EcoRI G'AATT_C Cuts at: 0 820 1686 Size: 820 866 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1120 1686 Size: 1120 566 HaeII r_GCGC'y Cuts at: 0 769 1686 Size: 769 917 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 156 188 489 521 1345 1377 1686 Size: 156 32 301 32 824 32 309 Fragments arranged by size: 824 309 301 156 32 32 32 HincII GTy'rAC Cuts at: 0 1683 1686 Size: 1683 3 HpaI GTT'AAC Cuts at: 0 1683 1686 Size: 1683 3 KasI G'GCGC_C Cuts at: 0 765 1686 Size: 765 921 MfeI C'AATT_G Cuts at: 0 571 1686 Size: 571 1115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 814 1686 Size: 814 872 MscI TGG'CCA Cuts at: 0 1267 1686 Size: 1267 419 MslI CAynn'nnrTG Cuts at: 0 466 1686 Size: 466 1220 MspA1I CmG'CkG Cuts at: 0 1173 1327 1686 Size: 1173 154 359 Fragments arranged by size: 1173 359 154 NarI GG'CG_CC Cuts at: 0 766 1686 Size: 766 920 NsiI A_TGCA'T Cuts at: 0 23 1686 Size: 23 1663 NspI r_CATG'y Cuts at: 0 25 100 546 744 1686 Size: 25 75 446 198 942 Fragments arranged by size: 942 446 198 75 25 PflMI CCAn_nnn'nTGG Cuts at: 0 1263 1686 Size: 1263 423 PfoI T'CCnGG_A Cuts at: 0 1129 1686 Size: 1129 557 PpuMI rG'GwC_Cy Cuts at: 0 1286 1686 Size: 1286 400 PspOMI G'GGCC_C Cuts at: 0 1272 1335 1686 Size: 1272 63 351 Fragments arranged by size: 1272 351 63 PvuII CAG'CTG Cuts at: 0 1173 1686 Size: 1173 513 SacI G_AGCT'C Cuts at: 0 162 1194 1686 Size: 162 1032 492 Fragments arranged by size: 1032 492 162 ScaI AGT'ACT Cuts at: 0 303 1686 Size: 303 1383 SfoI GGC'GCC Cuts at: 0 767 1686 Size: 767 919 SmlI C'TyrA_G Cuts at: 0 240 1686 Size: 240 1446 SnaBI TAC'GTA Cuts at: 0 1654 1686 Size: 1654 32 SphI G_CATG'C Cuts at: 0 25 744 1686 Size: 25 719 942 Fragments arranged by size: 942 719 25 StuI AGG'CCT Cuts at: 0 1213 1686 Size: 1213 473 StyI C'CwwG_G Cuts at: 0 581 1214 1686 Size: 581 633 472 Fragments arranged by size: 633 581 472 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 268 1686 Size: 268 1418 TatI w'GTAC_w Cuts at: 0 169 301 1686 Size: 169 132 1385 Fragments arranged by size: 1385 169 132 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 394 426 1278 1310 1324 1356 1686 Size: 394 32 852 32 14 32 330 Fragments arranged by size: 852 394 330 32 32 32 14 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 939 1686 Size: 939 747 XmnI GAAnn'nnTTC Cuts at: 0 1043 1686 Size: 1043 643 ZraI GAC'GTC Cuts at: 0 1203 1686 Size: 1203 483 Enzymes that do cut: AatII AccI AclI AcuI AflIII AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BfrBI BglII Bme1580I BmgBI BmrI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BstEII BstXI BstYI Bsu36I BtgI CspCI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NsiI NspI PflMI PfoI PpuMI PspOMI PvuII SacI ScaI SfoI SmlI SnaBI SphI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BglI BlpI BmtI BplI BpmI BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI BtsI ClaI DraIII DrdI EagI EcoRV FseI FspI FspAI HindIII KpnI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI