################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII BbeI BclI BfrI BglII BsaBI BseAI BsiEI BsmI BspDI BspEI BspLU11I BsrDI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI HincII HindII HpaI KasI KspI MamI MroI NarI NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 758 GGTACC G^GTACC AccII 337 CGCG CG^CG AcyI 964 GGCGTC G[GA]^CG[CT]C AflIII 336 ACGCGT A^C[GA][CT]GT AocI 2370 CCTCAGG CC^T[ACGT]AGG ApaI 821 GGGCCC GGGCC^C Asp718I 758 GGTACC G^GTACC AspI 1927 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2268 CCTAGG C^CTAGG BamHI 2085 GGATCC G^GATCC BbrPI 193 CACGTG CAC^GTG BbvCI 2045 CCTCAGC CCTCAGC(-5/-2) BcgI 1695 GCAGTACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1248 GTATCC GTATCC(6/5) BglI 1735 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2268 CCTAGG C^CTAGG BlpI 1690 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1690 GCTGAGC GC^T[ACGT]AGC BsaAI 193 CACGTG [CT]AC^GT[GA] BsaHI 964 GGCGTC G[GA]^CG[CT]C BsiWI 339 CGTACG C^GTACG BsmBI 595 GAGACG CGTCTC(1/5) BspHI 21 TCATGA T^CATGA BsrBI 853 CCGCTC CCGCTC(-3/-3) BstBI 1615 TTCGAA TT^CGAA BstUI 337 CGCG CG^CG BstYI 2085 GGATCC [GA]^GATC[CT] Bsu36I 2370 CCTCAGG CC^T[ACGT]AGG CelII 1690 GCTGAGC GC^T[ACGT]AGC DraIII 1457 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2370 CCTCAGG CC^T[ACGT]AGG EcoRI 1951 GAATTC G^AATTC EcoT22I 2700 ATGCAT ATGCA^T HaeII 2645 AGCGCC [GA]GCGC^[CT] HgaI 965 GCGTC GACGC(5/10) Hin1I 964 GGCGTC G[GA]^CG[CT]C HindIII 1511 AAGCTT A^AGCTT KpnI 758 GGTACC GGTAC^C MfeI 1935 CAATTG C^AATTG MflI 2085 GGATCC [GA]^GATC[CT] MluI 336 ACGCGT A^CGCGT MunI 1935 CAATTG C^AATTG MvnI 337 CGCG CG^CG NaeI 1004 GCCGGC GCC^GGC NdeI 1292 CATATG CA^TATG NgoMIV 1004 GCCGGC G^CCGGC NsiI 2700 ATGCAT ATGCA^T NspV 1615 TTCGAA TT^CGAA PflFI 1927 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1410 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 193 CACGTG CAC^GTG PmlI 193 CACGTG CAC^GTG PpuMI 2513 AGGACCC [GA]G^G[AT]CC[CT] RcaI 21 TCATGA T^CATGA SacI 1042 GAGCTC GAGCT^C SfuI 1615 TTCGAA TT^CGAA SmaI 811 CCCGGG CCC^GGG StuI 450 AGGCCT AGG^CCT Tth111I 1927 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1410 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 303 TCTAGA T^CTAGA XcmI 2582 CCAAGCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 2085 GGATCC [GA]^GATC[CT] XmaCI 811 CCCGGG C^CCGGG XmaI 811 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Alw44I 196 GTGCAC G^TGCAC 514 GTGCAC ApaLI 196 GTGCAC G^TGCAC 514 GTGCAC AvaI 811 CCCGGG C^[CT]CG[GA]G 2245 CCCGAG BsaI 1021 GAGACC GGTCTC(1/5) 2541 GGTCTC BsaWI 675 ACCGGA [AT]^CCGG[AT] 761 ACCGGA BsoBI 811 CCCGGG C^[CT]CG[GA]G 2245 CCCGAG Bsp1407I 947 TGTACA T^GTACA 2651 TGTACA BspMI 578 ACCTGC ACCTGC(4/8) 2708 ACCTGC BsrGI 947 TGTACA T^GTACA 2651 TGTACA CfoI 901 GCGC GCG^C 2646 GCGC EcoNI 2678 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2766 CCTACAAGAGG FauI 824 CCCGC CCCGC(4/6) 1882 CCCGC HhaI 901 GCGC GCG^C 2646 GCGC HinP1I 901 GCGC G^CGC 2646 GCGC MseI 431 TTAA T^TAA 1388 TTAA NcoI 1133 CCATGG C^CATGG 1639 CCATGG SexAI 539 ACCTGGT A^CC[AT]GGT 1661 ACCTGGT SspBI 947 TGTACA T^GTACA 2651 TGTACA Tru9I 431 TTAA T^TAA 1388 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I