(Linear) (Six-base) MAPSORT of: KIEE5813.seq Check: 8002 from: 1 to: 2778 KIEE5813.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. Acc65I G'GTAC_C Cuts at: 0 758 2778 Size: 758 2020 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 128 334 590 764 1264 2203 2778 Size: 113 15 206 256 174 500 939 575 Fragments arranged by size: 939 575 500 256 206 174 113 15 AflIII A'CryG_T Cuts at: 0 336 2778 Size: 336 2442 AleI CACnn'nnGTG Cuts at: 0 1772 2778 Size: 1772 1006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2725 2778 Size: 2725 53 AlwNI CAG_nnn'CTG Cuts at: 0 1239 1962 2107 2241 2778 Size: 1239 723 145 134 537 Fragments arranged by size: 1239 723 537 145 134 ApaI G_GGCC'C Cuts at: 0 825 2778 Size: 825 1953 ApaLI G'TGCA_C Cuts at: 0 196 514 2778 Size: 196 318 2264 Fragments arranged by size: 2264 318 196 ApoI r'AATT_y Cuts at: 0 421 645 719 1384 1951 2778 Size: 421 224 74 665 567 827 Fragments arranged by size: 827 665 567 421 224 74 AvaI C'yCGr_G Cuts at: 0 811 2245 2778 Size: 811 1434 533 Fragments arranged by size: 1434 811 533 AvrII C'CTAG_G Cuts at: 0 2268 2778 Size: 2268 510 BamHI G'GATC_C Cuts at: 0 2085 2778 Size: 2085 693 BanI G'GyrC_C Cuts at: 0 465 758 1128 2176 2778 Size: 465 293 370 1048 602 Fragments arranged by size: 1048 602 465 370 293 BanII G_rGCy'C Cuts at: 0 479 811 825 1046 1822 2778 Size: 479 332 14 221 776 956 Fragments arranged by size: 956 776 479 332 221 14 BbsI GAAGACnn'nnnn_ Cuts at: 0 578 1108 1625 2778 Size: 578 530 517 1153 Fragments arranged by size: 1153 578 530 517 BbvCI CC'TCA_GC Cuts at: 0 2046 2778 Size: 2046 732 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1684 1718 2778 Size: 1684 34 1060 Fragments arranged by size: 1684 1060 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1259 2778 Size: 1259 1519 BfrBI ATG'CAT Cuts at: 0 2702 2778 Size: 2702 76 BglI GCCn_nnn'nGGC Cuts at: 0 1741 2778 Size: 1741 1037 BlpI GC'TnA_GC Cuts at: 0 1691 2778 Size: 1691 1087 Bme1580I G_kGCm'C Cuts at: 0 200 518 825 2778 Size: 200 318 307 1953 Fragments arranged by size: 1953 318 307 200 BmgBI CAC'GTC Cuts at: 0 981 2778 Size: 981 1797 BmrI ACTGGGnnnn_n' Cuts at: 0 1809 2778 Size: 1809 969 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 392 2447 2778 Size: 392 2055 331 Fragments arranged by size: 2055 392 331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 859 1132 2129 2135 2211 2778 Size: 216 643 273 997 6 76 567 Fragments arranged by size: 997 643 567 273 216 76 6 Bpu10I CC'TnA_GC Cuts at: 0 805 1652 2046 2231 2778 Size: 805 847 394 185 547 Fragments arranged by size: 847 805 547 394 185 BsaI GGTCTCn'nnnn_ Cuts at: 0 1015 2547 2778 Size: 1015 1532 231 Fragments arranged by size: 1532 1015 231 BsaAI yAC'GTr Cuts at: 0 195 2778 Size: 195 2583 BsaHI Gr'CG_yC Cuts at: 0 965 2778 Size: 965 1813 BsaWI w'CCGG_w Cuts at: 0 675 761 2778 Size: 675 86 2017 Fragments arranged by size: 2017 675 86 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 783 813 2635 2665 2778 Size: 783 30 1822 30 113 Fragments arranged by size: 1822 783 113 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 523 2005 2778 Size: 19 504 1482 773 Fragments arranged by size: 1482 773 504 19 BseYI C'CCAG_C Cuts at: 0 438 556 903 993 2033 2038 2399 2657 Size: 438 118 347 90 1040 5 361 258 Cuts at: 2657 2778 Size: 121 Fragments arranged by size: 1040 438 361 347 258 121 118 90 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 260 642 719 2554 2778 Size: 260 382 77 1835 224 Fragments arranged by size: 1835 382 260 224 77 BsiHKAI G_wGCw'C Cuts at: 0 200 518 928 1046 1145 2778 Size: 200 318 410 118 99 1633 Fragments arranged by size: 1633 410 318 200 118 99 BsiWI C'GTAC_G Cuts at: 0 339 2778 Size: 339 2439 BsmBI CGTCTCn'nnnn_ Cuts at: 0 589 2778 Size: 589 2189 Bsp1286I G_dGCh'C Cuts at: 0 200 479 518 811 825 928 1046 1145 Size: 200 279 39 293 14 103 118 99 Cuts at: 1145 1822 2778 Size: 677 956 Fragments arranged by size: 956 677 293 279 200 118 103 99 39 14 BspHI T'CATG_A Cuts at: 0 21 2778 Size: 21 2757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 587 2717 2778 Size: 587 2130 61 Fragments arranged by size: 2130 587 61 BsrBI CCG'CTC Cuts at: 0 855 2778 Size: 855 1923 BsrFI r'CCGG_y Cuts at: 0 1004 2614 2630 2778 Size: 1004 1610 16 148 Fragments arranged by size: 1610 1004 148 16 BsrGI T'GTAC_A Cuts at: 0 947 2651 2778 Size: 947 1704 127 Fragments arranged by size: 1704 947 127 BstAPI GCAn_nnn'nTGC Cuts at: 0 285 1239 2241 2778 Size: 285 954 1002 537 Fragments arranged by size: 1002 954 537 285 BstBI TT'CG_AA Cuts at: 0 1616 2778 Size: 1616 1162 BstXI CCAn_nnnn'nTGG Cuts at: 0 1321 1680 2259 2361 2778 Size: 1321 359 579 102 417 Fragments arranged by size: 1321 579 417 359 102 BstYI r'GATC_y Cuts at: 0 2085 2778 Size: 2085 693 Bsu36I CC'TnA_GG Cuts at: 0 2371 2778 Size: 2371 407 BtgI C'CryG_G Cuts at: 0 1133 1314 1639 1773 2300 2354 2778 Size: 1133 181 325 134 527 54 424 Fragments arranged by size: 1133 527 424 325 181 134 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2327 2778 Size: 2327 451 BtsI GCAGTG_nn' Cuts at: 0 1237 1632 1767 2469 2671 2778 Size: 1237 395 135 702 202 107 Fragments arranged by size: 1237 702 395 202 135 107 DraIII CAC_nnn'GTG Cuts at: 0 1462 2778 Size: 1462 1316 EaeI y'GGCC_r Cuts at: 0 545 1323 2549 2606 2694 2778 Size: 545 778 1226 57 88 84 Fragments arranged by size: 1226 778 545 88 84 57 EarI CTCTTCn'nnn_ Cuts at: 0 147 500 1408 2438 2778 Size: 147 353 908 1030 340 Fragments arranged by size: 1030 908 353 340 147 EcoICRI GAG'CTC Cuts at: 0 1044 2778 Size: 1044 1734 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 128 216 334 590 764 859 1132 Size: 113 15 88 118 256 174 95 273 Cuts at: 1132 1264 2129 2135 2203 2211 2778 Size: 132 865 6 68 8 567 Fragments arranged by size: 865 567 273 256 174 132 118 113 95 88 68 15 8 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 2682 2770 2778 Size: 2682 88 8 Fragments arranged by size: 2682 88 8 EcoO109I rG'GnC_Cy Cuts at: 0 821 2514 2724 2778 Size: 821 1693 210 54 Fragments arranged by size: 1693 821 210 54 EcoRI G'AATT_C Cuts at: 0 1951 2778 Size: 1951 827 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2172 2778 Size: 2172 606 HaeII r_GCGC'y Cuts at: 0 2649 2778 Size: 2649 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 358 390 670 702 935 967 1328 1360 Size: 358 32 280 32 233 32 361 32 Cuts at: 1360 1480 1512 2778 Size: 120 32 1266 Fragments arranged by size: 1266 361 358 280 233 120 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1511 2778 Size: 1511 1267 KpnI G_GTAC'C Cuts at: 0 762 2778 Size: 762 2016 MfeI C'AATT_G Cuts at: 0 1935 2778 Size: 1935 843 MluI A'CGCG_T Cuts at: 0 336 2778 Size: 336 2442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 713 1185 1289 1359 1697 2778 Size: 713 472 104 70 338 1081 Fragments arranged by size: 1081 713 472 338 104 70 MscI TGG'CCA Cuts at: 0 547 1325 2551 2608 2778 Size: 547 778 1226 57 170 Fragments arranged by size: 1226 778 547 170 57 MslI CAynn'nnrTG Cuts at: 0 399 1138 1195 1319 1606 1714 1772 1891 Size: 399 739 57 124 287 108 58 119 Cuts at: 1891 2359 2463 2491 2778 Size: 468 104 28 287 Fragments arranged by size: 739 468 399 287 287 124 119 108 104 58 57 28 MspA1I CmG'CkG Cuts at: 0 282 2050 2657 2778 Size: 282 1768 607 121 Fragments arranged by size: 1768 607 282 121 NaeI GCC'GGC Cuts at: 0 1006 2778 Size: 1006 1772 NcoI C'CATG_G Cuts at: 0 1133 1639 2778 Size: 1133 506 1139 Fragments arranged by size: 1139 1133 506 NdeI CA'TA_TG Cuts at: 0 1293 2778 Size: 1293 1485 NgoMIV G'CCGG_C Cuts at: 0 1004 2778 Size: 1004 1774 NsiI A_TGCA'T Cuts at: 0 2704 2778 Size: 2704 74 NspI r_CATG'y Cuts at: 0 1537 1605 2328 2778 Size: 1537 68 723 450 Fragments arranged by size: 1537 723 450 68 PasI CC'CwG_GG Cuts at: 0 870 2778 Size: 870 1908 PflMI CCAn_nnn'nTGG Cuts at: 0 1416 2778 Size: 1416 1362 PfoI T'CCnGG_A Cuts at: 0 1791 2088 2778 Size: 1791 297 690 Fragments arranged by size: 1791 690 297 PmlI CAC'GTG Cuts at: 0 195 2778 Size: 195 2583 PpuMI rG'GwC_Cy Cuts at: 0 2514 2778 Size: 2514 264 PspOMI G'GGCC_C Cuts at: 0 821 2778 Size: 821 1957 PstI C_TGCA'G Cuts at: 0 299 351 1235 1627 2006 2778 Size: 299 52 884 392 379 772 Fragments arranged by size: 884 772 392 379 299 52 PvuII CAG'CTG Cuts at: 0 282 2050 2657 2778 Size: 282 1768 607 121 Fragments arranged by size: 1768 607 282 121 SacI G_AGCT'C Cuts at: 0 1046 2778 Size: 1046 1732 SexAI A'CCwGG_T Cuts at: 0 539 1661 2778 Size: 539 1122 1117 Fragments arranged by size: 1122 1117 539 SfcI C'TryA_G Cuts at: 0 295 347 1231 1623 2002 2778 Size: 295 52 884 392 379 776 Fragments arranged by size: 884 776 392 379 295 52 SmaI CCC'GGG Cuts at: 0 813 2778 Size: 813 1965 StuI AGG'CCT Cuts at: 0 452 2778 Size: 452 2326 StyI C'CwwG_G Cuts at: 0 1117 1133 1639 1985 2012 2207 2268 2534 Size: 1117 16 506 346 27 195 61 266 Cuts at: 2534 2778 Size: 244 Fragments arranged by size: 1117 506 346 266 244 195 61 27 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 763 1146 2400 2649 2653 2778 Size: 763 383 1254 249 4 125 Fragments arranged by size: 1254 763 383 249 125 4 TatI w'GTAC_w Cuts at: 0 947 1280 1697 2651 2778 Size: 947 333 417 954 127 Fragments arranged by size: 954 947 417 333 127 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 783 815 1420 1452 2379 2411 2778 Size: 783 32 605 32 927 32 367 Fragments arranged by size: 927 783 605 367 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1930 2778 Size: 1930 848 XbaI T'CTAG_A Cuts at: 0 303 2778 Size: 303 2475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2589 2778 Size: 2589 189 XmaI C'CCGG_G Cuts at: 0 811 2778 Size: 811 1967 Enzymes that do cut: Acc65I AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SexAI SfcI SmaI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI BaeI BbeI BclI BglII BmtI BpuEI BsaBI BsiEI BsmI BspEI BsrDI BssHII BssSI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoRV FseI FspI FspAI HincII HpaI KasI NarI NheI NotI NruI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI XhoI XmnI ZraI