################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII BbrPI BbvCI BcgI BciVI BclI BfrI BglII BsaAI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MroI MseI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1582 GTCTAC GT^[AC][GT]AC AcsI 261 GAATTC [GA]^AATT[CT] AcyI 1034 GGCGCC G[GA]^CG[CT]C AfaI 1391 GTAC GT^AC AflIII 556 ACATGT A^C[GA][CT]GT AhdI 900 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 261 GAATTC [GA]^AATT[CT] AspEI 900 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 920 GAGCAC G[AT]GC[AT]^C AspI 1555 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1321 CCTAGG C^CTAGG BalI 1631 TGGCCA TGG^CCA BamHI 889 GGATCC G^GATCC BbeI 1034 GGCGCC GGCGC^C BfaI 1322 CTAG C^TAG BglI 1516 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1321 CCTAGG C^CTAGG BpmI 1291 CTCCAG CTGGAG(16/14) BsaHI 1034 GGCGCC G[GA]^CG[CT]C BsaI 847 GAGACC GGTCTC(1/5) BsaWI 1077 ACCGGA [AT]^CCGG[AT] BsiHKAI 920 GAGCAC G[AT]GC[AT]^C BsmBI 538 CGTCTC CGTCTC(1/5) BspLU11I 556 ACATGT A^CATGT BsrBI 68 CCGCTC CCGCTC(-3/-3) BstYI 889 GGATCC [GA]^GATC[CT] DsaI 931 CCATGG C^C[GA][CT]GG Eam1105I 900 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 319 GAAGAG CTCTTC(1/4) EcoNI 48 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 261 GAATTC G^AATTC HaeII 1034 GGCGCC [GA]GCGC^[CT] Hin1I 1034 GGCGCC G[GA]^CG[CT]C KasI 1034 GGCGCC G^GCGCC Ksp632I 319 GAAGAG CTCTTC(1/4) MaeI 1322 CTAG C^TAG MflI 889 GGATCC [GA]^GATC[CT] MluNI 1631 TGGCCA TGG^CCA MscI 1631 TGGCCA TGG^CCA NarI 1034 GGCGCC GG^CGCC NcoI 931 CCATGG C^CATGG PflFI 1555 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 479 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 302 GACTC GAGTC(4/5) PshAI 590 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 1391 GTAC GT^AC SfoI 1034 GGCGCC GGC^GCC SphI 1341 GCATGC GCATG^C TfiI 667 GATTC G^A[AT]TC Tsp509I 262 AATT ^AATT Tth111I 1555 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 479 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 889 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 33 CGCG CG^CG 1536 CGCG AocI 112 CCTCAGG CC^T[ACGT]AGG 1129 CCTGAGG BbsI 59 GTCTTC GAAGAC(2/6) 1528 GTCTTC BlpI 70 GCTCAGC GC^T[ACGT]AGC 1612 GCTGAGC Bpu1102I 70 GCTCAGC GC^T[ACGT]AGC 1612 GCTGAGC BpuAI 59 GTCTTC GAAGAC(2/6) 1528 GTCTTC BsmAI 847 GAGAC GTCTC(1/5) 539 GTCTC BsrFI 1334 GCCGGC [GA]^CCGG[CT] 1521 GCCGGC BstUI 33 CGCG CG^CG 1536 CGCG Bsu36I 112 CCTCAGG CC^T[ACGT]AGG 1129 CCTGAGG CelII 70 GCTCAGC GC^T[ACGT]AGC 1612 GCTGAGC Cfr10I 1334 GCCGGC [GA]^CCGG[CT] 1521 GCCGGC DpnII 490 GATC ^GATC 890 GATC DrdI 205 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1573 GACTGGCAGGTC EaeI 1475 TGGCCG [CT]^GGCC[GA] 1631 TGGCCA Eco81I 112 CCTCAGG CC^T[ACGT]AGG 1129 CCTGAGG HgaI 757 GACGC GACGC(5/10) 537 GCGTC HinfI 302 GACTC G^A[ACGT]TC 667 GATTC MboI 490 GATC ^GATC 890 GATC MvnI 33 CGCG CG^CG 1536 CGCG NaeI 1334 GCCGGC GCC^GGC 1521 GCCGGC NdeII 490 GATC ^GATC 890 GATC NgoMIV 1334 GCCGGC G^CCGGC 1521 GCCGGC NspI 556 ACATGT [GA]CATG^[CT] 1341 GCATGC PaeR7I 691 CTCGAG C^TCGAG 809 CTCGAG Sau3AI 490 GATC ^GATC 890 GATC TaqI 692 TCGA T^CGA 810 TCGA Tsp45I 1174 GTGAC ^GT[GC]AC 1306 GTGAC TthHB8I 692 TCGA T^CGA 810 TCGA XhoI 691 CTCGAG C^TCGAG 809 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AlwI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PpuMI PstI PvuII Sau96I ScrFI SfaNI SfcI SmlI StyI TseI TspRI XcmI