(Circular) (Six-base) MAPSORT of: pFN21AE6456.seq Check: 3294 from: 1 to: 6364 pFN21AE6456 6364 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3240 3240 Size: 6364 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4174 278 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3588 3707 1937 Size: 1651 119 4594 Fragments arranged by size: 4594 1651 119 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3119 4427 5811 6079 1632 Size: 1487 1308 1384 268 1917 Fragments arranged by size: 1917 1487 1384 1308 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 2561 5263 6240 1203 Size: 1358 2702 977 1327 Fragments arranged by size: 2702 1358 1327 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 2910 5093 2910 Size: 2183 4181 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3359 1184 Size: 2175 4189 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2700 2732 2700 Size: 32 6332 AlwNI CAG_nnn'CTG Cuts at: 2138 2802 3356 4107 5679 2138 Size: 664 554 751 1572 2823 Fragments arranged by size: 2823 1572 751 664 554 ApaI G_GGCC'C Cuts at: 2024 2639 2660 2684 3271 2024 Size: 615 21 24 587 5117 Fragments arranged by size: 5117 615 587 24 21 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 2266 3673 3899 4051 6160 1493 Size: 773 1407 226 152 2109 1697 Fragments arranged by size: 2109 1697 1407 773 226 152 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 2696 2814 3611 3689 1870 Size: 73 753 118 797 78 4545 Fragments arranged by size: 4545 797 753 118 78 73 AvrII C'CTAG_G Cuts at: 3326 3326 Size: 6364 BamHI G'GATC_C Cuts at: 2894 3694 4061 2894 Size: 800 367 5197 Fragments arranged by size: 5197 800 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 3039 3442 3685 5140 Size: 325 217 627 255 997 403 243 1455 Cuts at: 5140 618 Size: 1842 Fragments arranged by size: 1842 1455 997 627 403 325 255 243 217 BanII G_rGCy'C Cuts at: 1391 2024 2182 2384 2639 2660 2684 2849 2992 Size: 633 158 202 255 21 24 165 143 Cuts at: 2992 3271 3612 3683 1391 Size: 279 341 71 4072 Fragments arranged by size: 4072 633 341 279 255 202 165 158 143 71 24 21 BbeI G_GCGC'C Cuts at: 3043 3043 Size: 6364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2057 3526 928 Size: 971 158 1469 3766 Fragments arranged by size: 3766 1469 971 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4555 4589 1903 Size: 34 2618 34 3678 Fragments arranged by size: 3678 2618 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4256 5466 4256 Size: 1210 5154 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3527 4975 136 Size: 107 122 71 631 605 1855 1448 1525 Fragments arranged by size: 1855 1525 1448 631 605 122 107 71 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 2076 3618 3781 2076 Size: 1542 163 4659 Fragments arranged by size: 4659 1542 163 Bme1580I G_kGCm'C Cuts at: 1790 2024 2639 2660 2684 2755 3271 3309 3445 Size: 234 615 21 24 71 516 38 136 Cuts at: 3445 3470 4425 5581 1790 Size: 25 955 1156 2573 Fragments arranged by size: 2573 1156 955 615 516 234 136 71 38 25 24 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6364 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2018 2722 3194 Size: 416 53 83 399 24 601 704 472 Cuts at: 3194 3571 4095 5053 442 Size: 377 524 958 1753 Fragments arranged by size: 1753 958 704 601 524 472 416 399 377 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3281 5024 1239 Size: 2042 1743 2579 Fragments arranged by size: 2579 2042 1743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2920 4489 5354 5652 2920 Size: 1569 865 298 3632 Fragments arranged by size: 3632 1569 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2846 5027 882 Size: 617 1347 2181 2219 Fragments arranged by size: 2219 2181 1347 617 BsaAI yAC'GTr Cuts at: 493 1204 4155 493 Size: 711 2951 2702 Fragments arranged by size: 2951 2702 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3040 4171 4553 5188 Size: 53 83 186 936 1507 1131 382 635 Cuts at: 5188 275 Size: 1451 Fragments arranged by size: 1507 1451 1131 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3082 4797 5195 5469 5616 6027 3082 Size: 1715 398 274 147 411 3419 Fragments arranged by size: 3419 1715 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2979 3009 1178 Size: 30 165 30 1576 30 4533 Fragments arranged by size: 4533 1576 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 3017 3101 1186 Size: 996 835 84 4449 Fragments arranged by size: 4449 996 835 84 BseYI C'CCAG_C Cuts at: 2016 2442 2478 2490 2640 3070 3171 3252 3419 Size: 426 36 12 150 430 101 81 167 Cuts at: 3419 5567 6063 2016 Size: 2148 496 2317 Fragments arranged by size: 2317 2148 496 430 426 167 150 101 81 36 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2570 2672 3473 1163 Size: 573 834 102 801 4054 Fragments arranged by size: 4054 834 801 573 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3814 4575 5603 1540 Size: 275 186 1813 761 1028 2301 Fragments arranged by size: 2301 1813 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2929 4425 4510 5581 2929 Size: 1496 85 1071 3712 Fragments arranged by size: 3712 1496 1071 85 BsmI GAATG_Cn' Cuts at: 3875 3968 3875 Size: 93 6271 BsmBI CGTCTCn'nnnn_ Cuts at: 2549 2549 Size: 6364 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2182 2384 2639 2660 2684 2755 Size: 399 234 158 202 255 21 24 71 Cuts at: 2755 2849 2929 2992 3271 3309 3445 3470 3612 Size: 94 80 63 279 38 136 25 142 Cuts at: 3612 3683 4425 4510 5581 1391 Size: 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 279 255 234 202 158 142 136 94 85 80 71 71 63 38 25 24 21 BspEI T'CCGG_A Cuts at: 6027 6027 Size: 6364 BspHI T'CATG_A Cuts at: 4253 5983 4253 Size: 1730 4634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2900 3240 3574 3719 844 Size: 2056 340 334 145 3489 Fragments arranged by size: 3489 2056 340 334 145 BsrBI CCG'CTC Cuts at: 1394 1823 2075 4251 1394 Size: 429 252 2176 3507 Fragments arranged by size: 3507 2176 429 252 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3339 3526 5008 5195 1700 Size: 110 1529 187 1482 187 2869 Fragments arranged by size: 2869 1529 1482 187 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6364 BssSI C'ACGA_G Cuts at: 4424 5436 4424 Size: 1012 5352 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstXI CCAn_nnnn'nTGG Cuts at: 2943 3057 3406 2943 Size: 114 349 5901 Fragments arranged by size: 5901 349 114 BstYI r'GATC_y Cuts at: 1972 2894 3694 4061 4446 4463 5915 6001 6013 Size: 922 800 367 385 17 1452 86 12 Cuts at: 6013 6359 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 922 800 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2118 3135 2118 Size: 1017 5347 BtgI C'CryG_G Cuts at: 513 1065 2936 513 Size: 552 1871 3941 Fragments arranged by size: 3941 1871 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2020 2438 487 Size: 683 56 794 418 4413 Fragments arranged by size: 4413 794 683 418 56 BtsI GCAGTG_nn' Cuts at: 2223 2233 3025 3880 4674 4694 2223 Size: 10 792 855 794 20 3893 Fragments arranged by size: 3893 855 794 792 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2216 3584 5371 809 Size: 507 900 1368 1787 1802 Fragments arranged by size: 1802 1787 1368 900 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3480 3636 3811 Size: 54 1425 50 275 18 1650 156 175 Cuts at: 3811 4700 6329 8 Size: 889 1629 43 Fragments arranged by size: 1650 1629 1425 889 275 175 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3811 1537 Size: 275 1999 4090 Fragments arranged by size: 4090 1999 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 4294 1353 Size: 966 1975 3423 Fragments arranged by size: 3423 1975 966 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3000 4951 5325 5471 387 Size: 1064 138 1411 1951 374 146 1280 Fragments arranged by size: 1951 1411 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3119 3281 4427 5024 5811 6079 1239 Size: 393 1487 162 1146 597 787 268 1524 Fragments arranged by size: 1524 1487 1146 787 597 393 268 162 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2057 Size: 6364 EcoO109I rG'GnC_Cy Cuts at: 2020 2218 2358 2520 2635 2636 2656 2657 2680 Size: 198 140 162 115 1 20 1 23 Cuts at: 2680 2681 2912 3046 3094 3323 3330 3430 3515 Size: 1 231 134 48 229 7 100 85 Cuts at: 3515 2020 Size: 4869 Fragments arranged by size: 4869 231 229 198 162 140 134 115 100 85 48 23 20 7 1 1 1 EcoRI G'AATT_C Cuts at: 2266 3673 4051 2266 Size: 1407 378 4579 Fragments arranged by size: 4579 1407 378 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 3043 5511 1678 Size: 1365 2468 2531 Fragments arranged by size: 2531 2468 1365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2169 2201 2896 2928 3270 Size: 32 135 32 597 32 695 32 342 Cuts at: 3270 3302 3419 3451 5005 5037 5079 5111 1373 Size: 32 117 32 1554 32 42 32 2626 Fragments arranged by size: 2626 1554 695 597 342 135 117 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3708 3954 669 Size: 988 281 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 3039 3039 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2628 5478 5662 1305 Size: 1323 2850 184 2007 Fragments arranged by size: 2850 2007 1323 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3638 10 Size: 54 1425 343 1806 2736 Fragments arranged by size: 2736 1806 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4322 4681 4840 518 Size: 648 3156 359 159 2042 Fragments arranged by size: 3156 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2193 2584 2789 2880 2974 3423 3660 3780 Size: 587 391 205 91 94 449 237 120 Cuts at: 3780 4457 5605 5850 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 587 449 391 245 237 205 120 94 91 NaeI GCC'GGC Cuts at: 3341 3528 3341 Size: 187 6177 NarI GG'CG_CC Cuts at: 3040 3040 Size: 6364 NcoI C'CATG_G Cuts at: 513 1065 2936 513 Size: 552 1871 3941 Fragments arranged by size: 3941 1871 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NgoMIV G'CCGG_C Cuts at: 3339 3526 3339 Size: 187 6177 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 2565 3350 3722 5267 1132 Size: 97 1336 785 372 1545 2229 Fragments arranged by size: 2229 1545 1336 785 372 97 PasI CC'CwG_GG Cuts at: 1348 2315 2355 2943 1348 Size: 967 40 588 4769 Fragments arranged by size: 4769 967 588 40 PciI A'CATG_T Cuts at: 2561 5263 2561 Size: 2702 3662 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2490 1108 Size: 902 480 4982 Fragments arranged by size: 4982 902 480 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 2520 2912 2218 Size: 302 392 5670 Fragments arranged by size: 5670 392 302 PshAI GACnn'nnGTC Cuts at: 1613 2599 1613 Size: 986 5378 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2020 2635 2656 2680 3267 2020 Size: 615 21 24 587 5117 Fragments arranged by size: 5117 615 587 24 21 PspXI vC'TCGA_Gb Cuts at: 2696 2814 2696 Size: 118 6246 PstI C_TGCA'G Cuts at: 830 2192 2298 2349 2403 2811 3338 3716 830 Size: 1362 106 51 54 408 527 378 3478 Fragments arranged by size: 3478 1362 527 408 378 106 54 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 PvuII CAG'CTG Cuts at: 2584 2789 2880 2974 3423 3660 2584 Size: 205 91 94 449 237 5288 Fragments arranged by size: 5288 449 237 205 94 91 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SanDI GG'GwC_CC Cuts at: 2520 2520 Size: 6364 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 2188 2294 2345 2371 2399 2807 3334 Size: 219 1143 106 51 26 28 408 527 Cuts at: 3334 3712 4847 5528 5719 826 Size: 378 1135 681 191 1471 Fragments arranged by size: 1471 1143 1135 681 527 408 378 219 191 106 51 28 26 SfoI GGC'GCC Cuts at: 3041 3041 Size: 6364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3691 1872 Size: 1819 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 2814 2899 4468 5369 5631 Size: 197 926 753 118 85 1569 901 262 Cuts at: 5631 820 Size: 1553 Fragments arranged by size: 1569 1553 926 901 753 262 197 118 85 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3350 3722 1132 Size: 2218 372 3774 Fragments arranged by size: 3774 2218 372 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6364 StyI C'CwwG_G Cuts at: 513 1065 2936 3326 3511 3803 513 Size: 552 1871 390 185 292 3074 Fragments arranged by size: 3074 1871 552 390 292 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3143 3398 4391 4408 4561 4746 5191 Size: 1255 1563 255 993 17 153 185 445 Cuts at: 5191 325 Size: 1498 Fragments arranged by size: 1563 1498 1255 993 445 255 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4610 6322 96 Size: 274 80 33 51 1443 2633 1712 138 Fragments arranged by size: 2633 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2614 2646 2979 3011 6324 6356 2614 Size: 32 333 32 3313 32 2622 Fragments arranged by size: 3313 2622 333 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3563 1316 Size: 2247 4117 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 3032 3236 2053 Size: 979 204 5181 Fragments arranged by size: 5181 979 204 XhoI C'TCGA_G Cuts at: 1943 2696 2814 1943 Size: 753 118 5493 Fragments arranged by size: 5493 753 118 XmaI C'CCGG_G Cuts at: 1870 3689 1870 Size: 1819 4545 XmnI GAAnn'nnTTC Cuts at: 4493 4493 Size: 6364 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4172 276 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI