(Linear) (Six-base) MAPSORT of: KIEE6456.seq Check: 2615 from: 1 to: 1659 KIEE6456.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1235 1659 Size: 1235 424 AccI GT'mk_AC Cuts at: 0 1583 1659 Size: 1583 76 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1114 1659 Size: 1114 545 AflIII A'CryG_T Cuts at: 0 556 1659 Size: 556 1103 AhdI GACnn_n'nnGTC Cuts at: 0 905 1659 Size: 905 754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1354 1659 Size: 1354 305 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 695 727 1659 Size: 695 32 932 Fragments arranged by size: 932 695 32 AlwNI CAG_nnn'CTG Cuts at: 0 133 797 1351 1659 Size: 133 664 554 308 Fragments arranged by size: 664 554 308 133 ApaI G_GGCC'C Cuts at: 0 19 634 655 679 1266 1659 Size: 19 615 21 24 587 393 Fragments arranged by size: 615 587 393 24 21 19 ApoI r'AATT_y Cuts at: 0 261 1659 Size: 261 1398 AvaI C'yCGr_G Cuts at: 0 691 809 1606 1659 Size: 691 118 797 53 Fragments arranged by size: 797 691 118 53 AvrII C'CTAG_G Cuts at: 0 1321 1659 Size: 1321 338 BamHI G'GATC_C Cuts at: 0 889 1659 Size: 889 770 BanI G'GyrC_C Cuts at: 0 37 1034 1437 1659 Size: 37 997 403 222 Fragments arranged by size: 997 403 222 37 BanII G_rGCy'C Cuts at: 0 19 177 379 634 655 679 844 987 Size: 19 158 202 255 21 24 165 143 Cuts at: 987 1266 1607 1659 Size: 279 341 52 Fragments arranged by size: 341 279 255 202 165 158 143 52 24 21 19 BbeI G_GCGC'C Cuts at: 0 1038 1659 Size: 1038 621 BbsI GAAGACnn'nnnn_ Cuts at: 0 52 1521 1659 Size: 52 1469 138 Fragments arranged by size: 1469 138 52 BglI GCCn_nnn'nGGC Cuts at: 0 1522 1659 Size: 1522 137 BlpI GC'TnA_GC Cuts at: 0 71 1613 1659 Size: 71 1542 46 Fragments arranged by size: 1542 71 46 Bme1580I G_kGCm'C Cuts at: 0 19 634 655 679 750 1266 1304 1440 Size: 19 615 21 24 71 516 38 136 Cuts at: 1440 1465 1659 Size: 25 194 Fragments arranged by size: 615 516 194 136 71 38 25 24 21 19 BmrI ACTGGGnnnn_n' Cuts at: 0 13 717 1189 1566 1659 Size: 13 704 472 377 93 Fragments arranged by size: 704 472 377 93 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1276 1659 Size: 1276 383 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 915 1659 Size: 915 744 BsaI GGTCTCn'nnnn_ Cuts at: 0 841 1659 Size: 841 818 BsaHI Gr'CG_yC Cuts at: 0 1035 1659 Size: 1035 624 BsaWI w'CCGG_w Cuts at: 0 1077 1659 Size: 1077 582 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 974 1004 1659 Size: 974 30 655 Fragments arranged by size: 974 655 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 177 1012 1096 1659 Size: 177 835 84 563 Fragments arranged by size: 835 563 177 84 BseYI C'CCAG_C Cuts at: 0 11 437 473 485 635 1065 1166 1247 Size: 11 426 36 12 150 430 101 81 Cuts at: 1247 1414 1659 Size: 167 245 Fragments arranged by size: 430 426 245 167 150 101 81 36 12 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 667 1468 1659 Size: 565 102 801 191 Fragments arranged by size: 801 565 191 102 BsiHKAI G_wGCw'C Cuts at: 0 924 1659 Size: 924 735 BsmBI CGTCTCn'nnnn_ Cuts at: 0 544 1659 Size: 544 1115 Bsp1286I G_dGCh'C Cuts at: 0 19 177 379 634 655 679 750 844 Size: 19 158 202 255 21 24 71 94 Cuts at: 844 924 987 1266 1304 1440 1465 1607 1659 Size: 80 63 279 38 136 25 142 52 Fragments arranged by size: 279 255 202 158 142 136 94 80 71 63 52 38 25 24 21 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 895 1235 1569 1659 Size: 895 340 334 90 Fragments arranged by size: 895 340 334 90 BsrBI CCG'CTC Cuts at: 0 70 1659 Size: 70 1589 BsrFI r'CCGG_y Cuts at: 0 1334 1521 1659 Size: 1334 187 138 Fragments arranged by size: 1334 187 138 BstXI CCAn_nnnn'nTGG Cuts at: 0 938 1052 1401 1659 Size: 938 114 349 258 Fragments arranged by size: 938 349 258 114 BstYI r'GATC_y Cuts at: 0 889 1659 Size: 889 770 Bsu36I CC'TnA_GG Cuts at: 0 113 1130 1659 Size: 113 1017 529 Fragments arranged by size: 1017 529 113 BtgI C'CryG_G Cuts at: 0 931 1659 Size: 931 728 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 15 433 1659 Size: 15 418 1226 Fragments arranged by size: 1226 418 15 BtsI GCAGTG_nn' Cuts at: 0 218 228 1020 1659 Size: 218 10 792 639 Fragments arranged by size: 792 639 218 10 DrdI GACnn_nn'nnGTC Cuts at: 0 211 1579 1659 Size: 211 1368 80 Fragments arranged by size: 1368 211 80 EaeI y'GGCC_r Cuts at: 0 1475 1631 1659 Size: 1475 156 28 Fragments arranged by size: 1475 156 28 EarI CTCTTCn'nnn_ Cuts at: 0 314 1659 Size: 314 1345 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 995 1659 Size: 995 664 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1114 1276 1659 Size: 1114 162 383 Fragments arranged by size: 1114 383 162 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 1659 Size: 52 1607 EcoO109I rG'GnC_Cy Cuts at: 0 15 213 353 515 630 631 651 652 Size: 15 198 140 162 115 1 20 1 Cuts at: 652 675 676 907 1041 1089 1318 1325 1425 Size: 23 1 231 134 48 229 7 100 Cuts at: 1425 1510 1659 Size: 85 149 Fragments arranged by size: 231 229 198 162 149 140 134 115 100 85 48 23 20 15 7 1 1 1 EcoRI G'AATT_C Cuts at: 0 261 1659 Size: 261 1398 HaeII r_GCGC'y Cuts at: 0 1038 1659 Size: 1038 621 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 164 196 891 923 1265 1297 1414 1446 Size: 164 32 695 32 342 32 117 32 Cuts at: 1446 1659 Size: 213 Fragments arranged by size: 695 342 213 164 117 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1034 1659 Size: 1034 625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 623 1659 Size: 623 1036 MscI TGG'CCA Cuts at: 0 1633 1659 Size: 1633 26 MspA1I CmG'CkG Cuts at: 0 188 579 784 875 969 1418 1655 1659 Size: 188 391 205 91 94 449 237 4 Fragments arranged by size: 449 391 237 205 188 94 91 4 NaeI GCC'GGC Cuts at: 0 1336 1523 1659 Size: 1336 187 136 Fragments arranged by size: 1336 187 136 NarI GG'CG_CC Cuts at: 0 1035 1659 Size: 1035 624 NcoI C'CATG_G Cuts at: 0 931 1659 Size: 931 728 NgoMIV G'CCGG_C Cuts at: 0 1334 1521 1659 Size: 1334 187 138 Fragments arranged by size: 1334 187 138 NspI r_CATG'y Cuts at: 0 560 1345 1659 Size: 560 785 314 Fragments arranged by size: 785 560 314 PasI CC'CwG_GG Cuts at: 0 310 350 938 1659 Size: 310 40 588 721 Fragments arranged by size: 721 588 310 40 PciI A'CATG_T Cuts at: 0 556 1659 Size: 556 1103 PflMI CCAn_nnn'nTGG Cuts at: 0 485 1659 Size: 485 1174 PpuMI rG'GwC_Cy Cuts at: 0 213 515 907 1659 Size: 213 302 392 752 Fragments arranged by size: 752 392 302 213 PshAI GACnn'nnGTC Cuts at: 0 594 1659 Size: 594 1065 PspOMI G'GGCC_C Cuts at: 0 15 630 651 675 1262 1659 Size: 15 615 21 24 587 397 Fragments arranged by size: 615 587 397 24 21 15 PspXI vC'TCGA_Gb Cuts at: 0 691 809 1659 Size: 691 118 850 Fragments arranged by size: 850 691 118 PstI C_TGCA'G Cuts at: 0 187 293 344 398 806 1333 1659 Size: 187 106 51 54 408 527 326 Fragments arranged by size: 527 408 326 187 106 54 51 PvuII CAG'CTG Cuts at: 0 579 784 875 969 1418 1655 1659 Size: 579 205 91 94 449 237 4 Fragments arranged by size: 579 449 237 205 94 91 4 SanDI GG'GwC_CC Cuts at: 0 515 1659 Size: 515 1144 SfcI C'TryA_G Cuts at: 0 183 289 340 366 394 802 1329 1659 Size: 183 106 51 26 28 408 527 330 Fragments arranged by size: 527 408 330 183 106 51 28 26 SfoI GGC'GCC Cuts at: 0 1036 1659 Size: 1036 623 SmlI C'TyrA_G Cuts at: 0 691 809 894 1659 Size: 691 118 85 765 Fragments arranged by size: 765 691 118 85 SphI G_CATG'C Cuts at: 0 1345 1659 Size: 1345 314 StyI C'CwwG_G Cuts at: 0 931 1321 1506 1659 Size: 931 390 185 153 Fragments arranged by size: 931 390 185 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1138 1393 1659 Size: 1138 255 266 Fragments arranged by size: 1138 266 255 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 609 641 974 1006 1659 Size: 609 32 333 32 653 Fragments arranged by size: 653 609 333 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1558 1659 Size: 1558 101 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 48 1027 1231 1659 Size: 48 979 204 428 Fragments arranged by size: 979 428 204 48 XhoI C'TCGA_G Cuts at: 0 691 809 1659 Size: 691 118 850 Fragments arranged by size: 850 691 118 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PpuMI PshAI PspOMI PspXI PstI PvuII SanDI SfcI SfoI SmlI SphI StyI TaqII TstI Tth111I XcmI XhoI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI ApaLI AscI AseI AsiSI BaeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII EagI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI XmaI XmnI ZraI