Restriction map for
pFN21AE4143
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BbrPI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MfeI MluI
MroI MseI MslI MunI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SspBI SspI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 801 GTCTAC GT^[AC][GT]AC
AfaI 466 GTAC GT^AC
AhdI 70 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1449 CCTGAGG CC^T[ACGT]AGG
ApaI 1286 GGGCCC GGGCC^C
AspEI 70 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1652 TGCGCA TGC^GCA
BalI 1373 TGGCCA TGG^CCA
BfaI 1615 CTAG C^TAG
BsaBI 1329 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 49 CGGCCG CG[GA][CT]^CG
BsrDI 968 GCAATG GCAATG(2/0)
BssSI 436 CACGAG CACGAG(-5/-1)
BstEII 1098 GGTCACC G^GT[ACGT]ACC
BstPI 1098 GGTCACC G^GT[ACGT]ACC
Bsu36I 1449 CCTGAGG CC^T[ACGT]AGG
DraIII 867 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 49 CGGCCG C^GGCCG
Eam1105I 70 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 781 GAAGAG CTCTTC(1/4)
EclXI 49 CGGCCG C^GGCCG
Eco52I 49 CGGCCG C^GGCCG
Eco81I 1449 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1098 GGTCACC G^GT[ACGT]ACC
FspI 1652 TGCGCA TGC^GCA
Ksp632I 781 GAAGAG CTCTTC(1/4)
KspI 23 CCGCGG CCGC^GG
MaeI 1615 CTAG C^TAG
MamI 1329 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 781 GAAGA GAAGA(8/7)
MluNI 1373 TGGCCA TGG^CCA
MscI 1373 TGGCCA TGG^CCA
NaeI 122 GCCGGC GCC^GGC
NcoI 473 CCATGG C^CATGG
NgoMIV 122 GCCGGC G^CCGGC
NruI 930 TCGCGA TCG^CGA
NspI 555 GCATGC [GA]CATG^[CT]
PpuMI 911 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 466 GTAC GT^AC
SacII 23 CCGCGG CCGC^GG
SbfI 1602 CCTGCAGG CCTGCA^GG
SgrAI 14 CGCCGGTG C[GA]^CCGG[CT]G
SphI 555 GCATGC GCATG^C
Sse8387I 1602 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 545 GACGTC GACGT^C
1684 GACGTC
BamHI 603 GGATCC G^GATCC
1622 GGATCC
BbeI 145 GGCGCC GGCGC^C
1671 GGCGCC
BglI 658 GCCGCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
755 GCCGCCAGGGC
BsaI 1555 GAGACC GGTCTC(1/5)
1636 GAGACC
BsrFI 15 GCCGGT [GA]^CCGG[CT]
122 GCCGGC
BsrI 63 ACTGG ACTGG(1/-1)
1559 CCAGT
BstAPI 1026 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1251 GCAGCAGCTGC
Cfr10I 15 GCCGGT [GA]^CCGG[CT]
122 GCCGGC
EcoNI 309 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1002 CCTGGCCCAGG
HgaI 1341 GACGC GACGC(5/10)
923 GCGTC
KasI 145 GGCGCC G^GCGCC
1671 GGCGCC
MaeII 546 ACGT A^CGT
1685 ACGT
MaeIII 1099 GTCAC ^GT[ACGT]AC
1339 GTGAC
NarI 145 GGCGCC GG^CGCC
1671 GGCGCC
SacI 115 GAGCTC GAGCT^C
943 GAGCTC
SfoI 145 GGCGCC GGC^GCC
1671 GGCGCC
SmaI 389 CCCGGG CCC^GGG
599 CCCGGG
StuI 665 AGGCCT AGG^CCT
1433 AGGCCT
TaqI 488 TCGA T^CGA
1331 TCGA
TfiI 528 GATTC G^A[AT]TC
1222 GAATC
Tsp45I 1099 GTCAC ^GT[GC]AC
1339 GTGAC
TthHB8I 488 TCGA T^CGA
1331 TCGA
XmaCI 389 CCCGGG C^CCGGG
599 CCCGGG
XmaI 389 CCCGGG C^CCGGG
599 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MboI MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI TspRI
XcmI XhoII